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  • 1
    Electronic Resource
    Electronic Resource
    Culturability and survival of an extreme thermophile isolated from deep-sea hydrothermal vents (1996)
    Springer
    Archives of microbiology 166 (1996), S. 64-67 
    Details
    ISSN: 1432-072X
    Keywords: Key wordsThermococcus peptonophilus ; Deep-sea ; Hydrothermal vents ; Survival ; Extreme thermophiles ; Culturability ; Active cells ; Mortality ; Dead cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The culturability of a strictly anaerobic, extremely thermophilic archaeon, Thermococcus peptonophilus (optimal growth temperature: 85° C), was studied during survival stages at various temperatures (98, 85, 70, and 4° C). Total cell number (determined by DAPI staining), active cells (rhodamine-stained cells), and culturable cells (using most-probable-number) were counted over time. The number of culturable cells decreased under each condition tested. The total number of cells significantly decreased only at temperatures close to the maximum for growth (98° C); at this temperature, the cells spontaneously lysed. Our results suggested that survival at 4° C in oxygenated waters might be a mechanism for the dispersion of extreme thermophiles in the ocean. In addition, we proved the existence of T. peptonophilus cells in several physiological states: culturable cells, active non-culturable cells, inactive non-culturable cells, and dead cells. Cell death was caused by cellular lysis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002030050356
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  • 2
    Electronic Resource
    Electronic Resource
    Pressure and temperature effects on growth and viability of the hyperthermophilic archaeon Thermococcus peptonophilus (1997)
    Springer
    Archives of microbiology 168 (1997), S. 1-7 
    Details
    ISSN: 1432-072X
    Keywords: Key wordsThermococcus peptonophilus ; Extreme thermophiles ; Large-scale cultivation ; Hydrostatic ; pressure ; Barophiles
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We studied the effects of high temperatures and elevated hydrostatic pressures on the physiological behavior and viability of the extremely thermophilic deep-sea archaeon Thermococcus peptonophilus. Maximal growth rates were observed at 30 and 45 MPa although no significant increases in cell yields were detected. Growth at 60 MPa was slower. The optimal growth temperature shifted from 85° C at 30 MPa to 90–95° C at 45 MPa. Cell viability during the stationary phase was also enhanced under high pressure. A trend towards barophily at pressures greater than those encountered in situ at the sea floor was demonstrated at increasing growth temperatures. The viability of cells during starvation, at high temperature (90, 95° C), and at low temperature (10° C) was enhanced at 30 and 45 MPa as compared to atmospheric pressure. These results show that the extremely thermophilic archaeon T. peptonophilus is a barophile.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002030050462
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Production of propionic acid by microbiological way. Part 1. Influence of the initial sugars and product concentrations (1992)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 12 (1992), S. 269-276 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The best yields and productivities of 0.38 g · g-1 and 0.35 g · l-1 h-1, respectively, for the propionic acid production in a batchsystem using sugar-cane final molasses as carbon source were obtained when an initial TRS concentration of 50 g · l-1 was used.It was obvious that this process is severely inhibited by the acids produced and the most drastic effect (μ = 0) was at a TVA concentration near to 250 mmol · l-1, independently of the initial TRS concentration employed.A generalizated equation of noncompetitive inhibition was adjusted: \documentclass{article}\pagestyle{empty}\begin{document}$$\mu = \frac{{\mu _{\max } }}{{1 + \left( {\frac{{TVA}}{{K_{\rm P} }}} \right)^n }}$$\end{document} and kinetic inhibition constants for each initial TRS concentration studied were estimated.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370120403
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  • 4
    Electronic Resource
    Electronic Resource
    The influence of MAILLARD's reaction in sugar cane molasses on the kinetic fermentation parameters of Candida utilis NRRL Y-660 (1994)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 14 (1994), S. 53-59 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Growth inhibition of Candida utilis NRRL Y-660 took place in molasses stored at 60°C for 120 days. The specific growth rate (μmax) was reduced from 0.42 h-1 to 0.200 h-1 as a result of a lack of affinity from the microorganism to the substrate and the increasing maintenance necessities. The Ks values arose from 1.40 mg/ml to 4.28 mg/ml within the whole experiment. At the same time, the maintenance coefficient (m) increased from 0.250 to 3.80 mg/ml. In a continuous culture the “wash-out” conditions were reached at dilution rate values (D) close to 0.40 h-1. The process productivity decreased up to 15% from its original value in fresh molasses.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370140109
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