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  • Articles: DFG German National Licenses  (6)
  • Lectins  (3)
  • Muscle contraction  (3)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 62 (1982), S. 263-271 
    ISSN: 1432-2242
    Keywords: Phaseolus vulgaris ; Seed protein ; Lectins ; Electrophoresis ; Agglutination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Single seeds of over 100 bean cultivars were analyzed by two-dimensional electrophoresis. The cultivars could be classified into eight groups by virtue of their G2/albumin electrophoretic patterns: TG2, SG2, VG2, PrG2, BG2, MG2, PG2, and PiG2, The polypeptide compositions of these types were largely inter-related having particular polypeptides in common. It was possible to correlate the G2/albumin patterns with agglutinating activity of cow and rabbit blood cells as measured by the agglutination ratio (minimum concentration of extract required to agglutinate cow blood cells: minimum concentration of extract required to agglutinate rabbit blood cells). The active lectin polypeptides were identified by extracting lectins from agglutinated erythrocytes and by comparing the qualitative similarities and differences of the G2/albumin patterns and their agglutination activities. A reference catalogue of over 100 bean cultivars giving their phaseolin and G2/albumin electrophoretic patterns, and agglutination ratios is presented.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 62 (1982), S. 361-367 
    ISSN: 1432-2242
    Keywords: Phaseolus vulgaris ; Lectins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The relationship between the polypeptide composition and the agglutination behaviour of the lectin-containing G2/albumin protein groups has allowed the identification of the active lectin polypeptides in different cultivars of Phaseolus vulgaris (Brown et al. accompanying paper). These results were used to ascertain the particular G2/albumin group contained in the various lectin sources used previously for the purification of lectin proteins. Many studies were found to have included lectin sources which contained the same G2/albumin pattern (TG2) and this common denominator has permitted the direct comparison of the properties reported for these purified lectins. Thus, much of the extensive literature on bean lectins is concurred.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-2242
    Keywords: Phaseolus vulgaris ; Lectins ; Albumin ; Globulin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Variation in the native conformation of bean lectins was examined using electrophoresis of non-denatured total protein extracts and purified albumin and globulin lectin. The observed variation was related to the genetic variation reported previously for lectin polypeptide composition as revealed by two-dimensional isoelectricfocusing-sodium dodecyl sulfate polyacrylamide gel electrophoresis (IEF-SDS/PAGE). When eleven cultivars with different IEF-SDS/PAGE lectin polypeptide compositions were compared, eight had unique non-denatured lectin patterns and three had identical patterns. For some cultivars differences in non-denatured lectin patterns were observed between the purified albumin and globulin lectin preparations.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    European journal of applied physiology 69 (1994), S. 344-349 
    ISSN: 1439-6327
    Keywords: Aging ; Electromyography ; Reaction time ; Motor control ; Muscle contraction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The aim of this investigation was to determine whether age-related changes in the dynamics of muscle activation were, in part, responsible for longer reaction times (RT) in the elderly. A group of 12 young (mean age, 20.6 years) and 12 elderly (mean age, 64.3 years) women performed a series of ballistic forearm supination movements in response to an auditory stimulus while using a simple reaction time test. Surface electromyographic waveforms from biceps brachii (agonist) and pronator teres (antagonist) muscles were recorded, together with the angle-time curves representing the motion of the forearm, on to an IBM compatible microcomputer. The results showed that an age-related increase (P〈0.05) in motor reaction time (MRT) contributed to longer RT in the elderly. In addition, the longer (P〈0.05) MRTs in the elderly were associated with a significantly slower rate (P〈0.05) of biceps brachii muscle activation and a significantly increased proportion (P〈0.05) of the initial biceps brachii muscle burst required to initiate the movement. This data suggested that an important part of the slowing of motor behaviour, commonly observed with increasing age, may be due to either decreases in the ability of aged skeletal muscle to rapidly generate tension or to a reduction in motor drive.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    European journal of applied physiology 62 (1991), S. 162-170 
    ISSN: 1439-6327
    Keywords: Motor control ; Electromyography ; Muscle contraction ; Inaccurate movement ; Motor skill
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The electromyographic basis of inaccurate performance was investigated in two rapid precision-grip skills controlled by concentric and eccentric muscle contractions respectively. Surface electromyograms, recorded from the first dorsal interosseous (DI), adductor pollicis (AP) and abductor pollicis brevis, were utilised to identify changes in the timing and intensity of muscle activation which may be responsible for inaccurate performance. The results showed that when fast precision-grip skills were controlled by concentric DI and AP muscle contractions, variations in the intensity of muscle contraction were responsible for inaccurate performance. However, when these skills were controlled by eccentric DI and AP muscle contractions, inaccurate performance resulted from variations in the timing of muscle activation. It was concluded that the nature of the deficiency in the patterns of muscle activation resulting in inaccurate performance was dependent upon the type of muscle contraction used in the skill.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    European journal of applied physiology 63 (1991), S. 381-386 
    ISSN: 1439-6327
    Keywords: Motor control ; Electromyography ; Motor skill ; Triphasic electromyogram ; Muscle contraction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The purpose of this investigation was to determine how the triphasic electromyogram (EMG) pattern of muscle activation developed from the agonist muscle only pattern as movement time (t mov) decreased. Six adult women produced a series of 30° elbow extension movements in the horizontal plane at speeds ranging from ballistic (〈 400-ms t mov) to very slow (〉 800-ms t mov). Surface EMG from triceps brachii (agonist) and biceps brachii (antagonist) muscles were recorded, together with elbow angle, on a microcomputer. The results showed that triphasic EMG patterns developed systematically as t mov decreased from 1000 ms to 〈 200 ms. In trials with very long t mov, many elbow extension movements were produced by a single continuous activation of the agonist triceps brachii muscle. As t mov decreased however, agonist activation became predominantly burst-like and other components of the triphasic EMG pattern [activation of the antagonist (Ant) and second agonist activation (Ag2)] began to appear. At the fastest movement speeds, triphasic EMG patterns (Ag1-Ant-Ag2, Ag1 being first activation of agonist muscle) were always present. This data indicated that the triphasic pattern of muscle activation was not switched on when a particular t mov was achieved. Rather, each component systematically developed until all were present, as distinctive bursts of activity, in most trials with t mov less than 400 ms.
    Type of Medium: Electronic Resource
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