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  • Life Sciences  (9)
  • 1
    Electronic Resource
    Electronic Resource
    Activity of diaminopimelic acid decarboxylase in bacteria producing lysine (1983)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 3 (1983), S. 217-220 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Activity of DAP-decarboxylase in lysine overproducing strains of Micrococcus luteus, M. varians and Arthrobacter globiformisLb reached the highest level during the end of the exponetial phase of growth and remained at the same high level during the stationary phase of growth when major bulk of lysine was accumulated. In comparison in a lysine non-producing strain of Arthrobacter globiformis I 4 the activity of the same enzyme was low. DAP-decarboxylase of these three lysine overproducers has two specialities, persistence during the stationary phase and insensivity to repression by exogenous lysine.
    Additional Material: 2 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370030303
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  • 2
    Electronic Resource
    Electronic Resource
    Influence of antibiotics and trace salts on lysine production by arthrobacter globiformis (1985)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 5 (1985), S. 215-218 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: A hydrocarbon utilizing strain of Arthrobacter globiformis Lb isolated from local soil has been found to yield lysine 3.4 g l-1, keeping the medium optimal for pH, C- and N-sources. Addition of antibiotics and micronutrients to that optimal media stimulated cell growth and enhanced lysine yield.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370050223
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  • 3
    Electronic Resource
    Electronic Resource
    Lysine production from hydrocarbon by micrococcus species (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 287-296 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: A bacterium isolated from Assam (India) soil was found to accumulate L-lysine in the mineral salt-hydrocarbon medium and identified to be a strain of Micrococcus luteus. The strain is able to grow and accumulate l-lysine in a purely synthetic medium, but supplementation of the synthetic medium with casamino acid or yeast extract or both, improves the yield. The entire fermentation period can be divided into a growth phase and a production phase, which can be prolonged by adjustment of the pH to the neutral range. Among the different hydrocarbon and nitrogen sources tested straight run gas oil (47percnt;) and ammonium sulphate (0.4%), respectively, were found most suitable.Erythromycin at 1 μg/ml level inhibited growth bu¸t stimulated lysine excretion. An inoculum level of 10% (v/v) of the medium was optimal for lysine production. The yield of lysine under optimal conditions was found to be 3.25 g per litre of the medium. Lysine has been isolated in crystalline form from the fermented broth by ion exchange resin chromatography and found to be pure sample of L-isomer.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020311
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  • 4
    Electronic Resource
    Electronic Resource
    Enhancement of methionine production by methionine analogue ethionine resistant mutants of Brevibacterium heali (1994)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 14 (1994), S. 199-204 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: In order to improve the methionine yield of the isolate B. heali, attempts were made to isolate mutants resistant to the methionine analogue DL-ethionine after mutagenesis with N-methyl-N′-nitro-N-nitrosoguanidine (NTG). The minimum inhibitory concentration (MIC) of ethionine for B. heali was found to be 2 mM. After mutagenesis and screening, five mutants resistant to 50 mM of ethionine were isolated. The yield of the best ethionine resistant mutant, B. heali Br EthR250, was 13 mg/l of methionine medium under optimum cultivation conditions.
    Additional Material: 4 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370140214
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  • 5
    Electronic Resource
    Electronic Resource
    L-Lysine production by auxotrophic mutants of Arthrobacter globiformis (1988)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 8 (1988), S. 527-533 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: By mutagenesis with N-methyl N-nitro-N-nitrosoguanidine, in two steps, a number of methionine plus threonine double auxotrophs have been isolated from a glutamate producing Arthrobacter globiformis, excreting L-lysine in good amounts. For the three potent mutants tested the medium of WHITE was adjudged to be the best. Biotin, ammonium chloride and glucose was found to be optimum at 5 μg l-1, 40 mM and 4% level, respectively. With such optimal C and N source, the strain MT 35 yielded 28.0 g lysine l-1 of medium in flask culture.
    Additional Material: 5 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370080612
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  • 6
    Electronic Resource
    Electronic Resource
    Production of l-phenylalanine by double auxotrophic mutants of arthrobacter globiformis: Optimization of c and n-source (1991)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 11 (1991), S. 249-254 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: A number of tryptophan plus tyrosine double auxotrophic mutants isolated by NTG treatment of a glutamic acid prodcing strain of A. globiformis were found to excrete phenylalanine alone in glucose, ammonium chloride, mineral salt medium. Among 24 different carbon sources tested glucose was found to be the best and optimum for -phenylalanine production was at 350 mM level. Among 14 different inorganic and organic nitrogen sources tested ammonium chloride was the best and was optimal at 60 mM level for phenylalanine production. On the optimum level of C and N sources a yield of 1.5 g/l phenylalanine was achieved.
    Additional Material: 4 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370110315
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  • 7
    Electronic Resource
    Electronic Resource
    L-Methionine production by double auxotrophic mutants of a ethionine resistant strain of Brevibacterium heali (1994)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 14 (1994), S. 61-66 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: A number of lysine plus threonine double auxotrophs have been isolated from a ethionine resistant methionine producing strain of Brevibacterium heali previously isolated from soil by mutagenesis with N-methyl N′-nitro-N-nitrosoguanidine in two steps. This strain excreted L-methionine in sufficient amounts. For the three potent mutants tested, the medium of ALFOLDI was judged to be the best. Biotin and ammonium nitrate were found to be optimal at 5 μg/l and at a 40 mM level, respectively. With such an optimal dose, the strain BhLT 27 yielded 25.5 g/l methionine in a flask culture containing methionine-analogue ethionine at a minimal inhibitory concentration.
    Additional Material: 4 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370140111
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  • 8
    Electronic Resource
    Electronic Resource
    Influence of B-vitamins and trace elements on lysine production by micrococcus varians 2Fa (1989)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 9 (1989), S. 63-67 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: A hydrocarbon utilizing strain of Micrococcus varians 2Fa isolated from local soil has been found to yield lysine 2.6 g l-1. Addition of B-vitamins and trace elements to the optimal media, has been found to stimulate growth and enhance lysine yield.
    Additional Material: 2 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370090111
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  • 9
    Electronic Resource
    Electronic Resource
    Dihydrodipicolinate synthase of lysine excreting and non excreting strains of bacillus megaterium (1990)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 10 (1990), S. 382-384 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Dihydrodipicolinate synthase activity of lysine producing strain of Bacillus megaterium and its high yielding mutant AECr 60f was highest during the end of the exponential phase and remained at the same level during the stationary phase when major bulk of lysine was accumulated. In comparision in a lysine non producing strain of B. megaterium the activity of the enzyme was low. Lysine producing strains has DHD synthase with two specialities, persistence during the stationary phase and insensitivity to repression by exogenous lysine. The high yielding mutant has increased DHD synthase activity which was not sensitive to feed back inhibition with lysine.
    Additional Material: 2 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370100414
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