ISSN:
0138-4988
Keywords:
Life Sciences
;
Life Sciences (general)
Source:
Wiley InterScience Backfile Collection 1832-2000
Topics:
Process Engineering, Biotechnology, Nutrition Technology
Notes:
By mutagenesis with N-methyl N-nitro-N-nitrosoguanidine, in two steps, a number of methionine plus threonine double auxotrophs have been isolated from a glutamate producing Arthrobacter globiformis, excreting L-lysine in good amounts. For the three potent mutants tested the medium of WHITE was adjudged to be the best. Biotin, ammonium chloride and glucose was found to be optimum at 5 μg l-1, 40 mM and 4% level, respectively. With such optimal C and N source, the strain MT 35 yielded 28.0 g lysine l-1 of medium in flask culture.
Additional Material:
5 Tab.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1002/abio.370080612
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