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Digitale Medien

L-Lysine production by auxotrophic mutants of Arthrobacter globiformis (1988)

Samanta, T. K. ; Das, Y. ; Mondal, S. ; [weitere]

Berlin : Wiley-Blackwell

Acta Biotechnologica 8 (1988), S. 527-533

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Details

ISSN: 0138-4988

Schlagwort(e): Life Sciences ; Life Sciences (general)

Quelle: Wiley InterScience Backfile Collection 1832-2000

Thema: Werkstoffwissenschaften, Fertigungsverfahren, Fertigung

Notizen: By mutagenesis with N-methyl N-nitro-N-nitrosoguanidine, in two steps, a number of methionine plus threonine double auxotrophs have been isolated from a glutamate producing Arthrobacter globiformis, excreting L-lysine in good amounts. For the three potent mutants tested the medium of WHITE was adjudged to be the best. Biotin, ammonium chloride and glucose was found to be optimum at 5 μg l-1, 40 mM and 4% level, respectively. With such optimal C and N source, the strain MT 35 yielded 28.0 g lysine l-1 of medium in flask culture.

Zusätzliches Material: 5 Tab.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1002/abio.370080612

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