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  • 1
    Electronic Resource
    Electronic Resource
    Nucleic acid reduction from yeast activation of intracellular rnase (1985)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 5 (1985), S. 91-100 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The study of a heat-shock process for RNA reduction was carried out for different yeast strains. Different results were obtained from each of them. Candida utilis NRRL Y-660 shows its best performance after a 8-s. heat-shock in the presence of 3% NaCl. For commercial baker's yeast Saccharomyces cerevisiae and Kluyveromyces fragilis L-1930, similar results were obtained with only 1% of NaCl. The latter needed longer heat-shock periods. e.g. 15s. to give such an RNA reduction. Biomass recovery ranged from 60 to 75%, being higher for C. utilis and K. fragilis while excessive losses were observed in S. cerevisiae cells.No significant protein deterioration was obtained in the best performance samples. The aminoacid profile appears to be improved in comparison to the starting material in these strains after RNA reduction.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370050116
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  • 2
    Electronic Resource
    Electronic Resource
    Cross flow filtration of RNA extracts by hollow fiber membrane (1992)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 12 (1992), S. 49-56 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The feasibility of using ultrafiltration to concentrate RNA was investigated. Ultrafiltration flux and solute retentivity were affected by membrane cut-off and inlet pressure.The RNA can be concentrated by selecting a hydraulically permeable membrane (PM 30) and then operating the system at a sufficiently high pressure (2.07 · 105 Nm-2) and a high recirculation rate (150 cm-1). The use of this procedure was limited to a high-flux regime. However, a very high retentivity of macrosolute was achieved. The effect of transmembrane pressure on the apparent mass transfer coefficient in the “gel polarization model” was discussed and related to ultrafiltration flux.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370120113
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Behaviour of Candida utilis during growth in relation to Mg2+ and K+ concentration in sugar cane molasses (1994)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 14 (1994), S. 111-114 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Magnesium and potassium ions are present in sugar cane molasses in a concentration of about 0.3% and 2.5%, respectively, which is high enough to support biomass production from Candida utilis. Culture broth with 40 g/l of total reducing substances supplemented by the addition of 1 ppm of Mg2+ leads to a higher yield (Y x/s) in batch fermentation experiments. The subsequent addition of Mg2+ up to 10 ppm decreases the yield coefficient from 0.53 to 0.42 without affecting the growth rate. Fermentation media supplemented with 1 to 10 ppm of K+ decreased both the yield coefficient and the specific growth rate. A Mg/K ratio of about 0.1 seems to be optimal for yeast biomass propagation.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370140118
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    RNA removal from Candida utilis by chemical and thermal treatments (1982)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 2 (1982), S. 145-153 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: The removal of the content of nucleic acids of fodder yeast (Candida utilis) by treatment with HCl or heat shock was investigatedAcid concentrations between 5 and 15% (on dried matter basis) were used. A maximal removal of the content of nucleic acids of 88% was realized wheńn was worked with 15% HCl, 90 °C during 30 minutes. But under this conditions were observed high demanges of protein and also of some essential amino acidsGood results for diminishing the content of nucleic acids were reached with the highest concentration of acid and a treatment during 20 minutesThe experiments with heat shock were carried out at 68 °C, different times for heating and different contents of yeastIn this way better results than for treatment with acid according to diminishing the content of nucleic acids and yield of protein and essential amino acids were reachedA removal of over 80% of the content of nucleic acids was achieved in all cases.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370020205
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    The influence of MAILLARD's reaction in sugar cane molasses on the kinetic fermentation parameters of Candida utilis NRRL Y-660 (1994)
    Berlin : Wiley-Blackwell
    Acta Biotechnologica 14 (1994), S. 53-59 
    Details
    ISSN: 0138-4988
    Keywords: Life Sciences ; Life Sciences (general)
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Process Engineering, Biotechnology, Nutrition Technology
    Notes: Growth inhibition of Candida utilis NRRL Y-660 took place in molasses stored at 60°C for 120 days. The specific growth rate (μmax) was reduced from 0.42 h-1 to 0.200 h-1 as a result of a lack of affinity from the microorganism to the substrate and the increasing maintenance necessities. The Ks values arose from 1.40 mg/ml to 4.28 mg/ml within the whole experiment. At the same time, the maintenance coefficient (m) increased from 0.250 to 3.80 mg/ml. In a continuous culture the “wash-out” conditions were reached at dilution rate values (D) close to 0.40 h-1. The process productivity decreased up to 15% from its original value in fresh molasses.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/abio.370140109
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    Paper (German National Licenses)
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