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  • Cell & Developmental Biology  (8)
  • Lycopersicon  (4)
  • Cell culture  (3)
  • 1
    Electronic Resource
    Electronic Resource
    In vitro culture of human prostatic tissue (1977)
    Springer
    Urological research 5 (1977), S. 207-210 
    Details
    ISSN: 1434-0879
    Keywords: Cell culture ; Prostate ; Collagenase ; Epithelial
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A procedure is described which yields a significant percentage of long-term mixed cell cultures of human prostatic tissue. Attempts were made to suppress the proliferation of stromal fibroblasts and to characterize the cultured cells as those of prostatic origin. The problems associated with establishing epithelial cell lines are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00263737
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  • 2
    Electronic Resource
    Electronic Resource
    Nitrate reductase activity in Paul's scarlet rose suspension cultures and the differential role of nitrate and molybdenum in induction (1978)
    Springer
    Planta 141 (1978), S. 183-189 
    Details
    ISSN: 1432-2048
    Keywords: Antimetabolites ; Cell culture ; Enzyme induction ; Molybdenum ; Nitrate reductase ; Rosa, Paul's Scarlet
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Induction of nitrate reductase (EC 1.6.6.1) activity was measured in Paul's Scarlet rose cell suspensions cultured in media containing nitrate (NO 3 - ) or urea (U) as nitrogen source, and with (+Mo) or without molybdenum (-Mo). There was a lag of 30 min during induction by NO 3 - in +Mo cultures but no lag occurred during induction after adding Mo to NO 3 - -Mo or to U-Mo cultures preincubated with NO 3 - . Actinomycin D, cycloheximide, and puromycin completely blocked induction by NO 3 - , but had no effect on the initial rate of induction by Mo. Cycloheximide and puromycin blocked induction by NO 3 - more quickly than actinomycin D. Induction by NO 3 - appeared to involve mRNA-dependent synthesis of apoprotein followed by rapid activation with molybdenum in intact cells independently of protein synthesis. Nitrate-induced apoprotein appeared less stable than the holoenzyme. When induced by NO 3 - in the absence of Mo, apoprotein concentration was about half the amount of maximally induced nitrate reductase. Cycloheximide stabilised preformed nitrate reductase which disappeared steadily in the presence of puromycin. Apoprotein was not stabilised by either antimetabolite.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00387887
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  • 3
    Electronic Resource
    Electronic Resource
    Nitrate reductase activity and growth in Paul's Scarlet rose suspension cultures in relation to nitrogen source and molybdenum (1976)
    Springer
    Planta 133 (1976), S. 27-34 
    Details
    ISSN: 1432-2048
    Keywords: Rose ; Paul's Scarlet ; Cell culture ; Nitrate reductase ; Molybdenum ; Enzyme induction ; Enzyme assay
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Growth and nitrate reductase activity were measured in Paul's Scarlet rose cell suspensions, cultured in media purified from molybdenum and containing nitrate or urea as sole nitrogen source with or without added Mo. Urea could replace nitrate to yield 80% of the fresh weight in nitrate medium. Nitrate reductase activities were compared by in vivo and in vitro assays. The latter varied due to inactivation during extraction. Compared with activities in cells in complete NO3 - medium, activity in NO3 --Mo cells was reduced to 30% and, in urea-grown cells, to trace amounts. Increases in nitrate reductase activity were found when NO3 - alone was added to NO3 - or urea+Mo cultures. In NO3 --Mo cultures, Mo alone or with NO3 - caused a similar increase in activity, whereas urea-Mo cultures required both NO3 - and Mo for enzyme induction.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00386002
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  • 4
    Electronic Resource
    Electronic Resource
    Low temperature effect on selective fertilization by pollen mixtures of wild and cultivated tomato species (1981)
    Springer
    Theoretical and applied genetics 59 (1981), S. 235-238 
    Details
    ISSN: 1432-2242
    Keywords: Low temperature adaptation ; Lycopersicon ; Pollen mixtures ; Selective fertilization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In vitro pollen germination of cultivated tomato, Lycopersicon esculentum Mill., is inhibited by an ambient temperature of 5°C, more so than pollen from a Peruvian ecotype of Lycopersicon hirsutum Humb. & Bonpl. originating from an altitude of 3200 m. The frequency of L. hirsutum gametes contributing to hybrid zygote formation is more than doubled when controlled fertilizations with pollen mixtures of the two species occurs at 12/6°C as compared to crosses with the same mixtures at 24/19°C. The results suggest that differential selection at the gametophytic level occurs in response to low temperature regimes. To our knowledge this is the first time in higher plants that alteration of an environmental factor has been demonstrated to change selection values of male gametophytes in a fashion predicted by the ecology of the parental sporophytes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00265501
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  • 5
    Electronic Resource
    Electronic Resource
    Mapping QTLs conferring salt tolerance during germination in tomato by selective genotyping (1997)
    Springer
    Molecular breeding 3 (1997), S. 269-277 
    Details
    ISSN: 1572-9788
    Keywords: Lycopersicon ; marker-assisted selection (MAS) ; quantitative trait loci (QTLs) ; restriction fragment length polymorphism (RFLP) ; salt tolerance ; seed germination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract This study was conducted to identify genomic regions (quantitative trait loci, QTLs) affecting salt tolerance during germination in tomato. Germination response of an F2 population of a cross between UCT5 (Lycopersicon esculentum, salt-sensitive) and LA716 (L. pennellii, salt-tolerant) was evaluated at a salt-stress level of 175 mM NaCl + 17.5 mM CaCl2 (water potential ca. −950 kPa). Germination was scored visually as radicle protrusion at 6 h intervals for 30 consecutive days. Individuals at both extremes of the response distribution (i.e., salt-tolerant and salt-sensitive individuals) were selected. The selected individuals were genotyped at 84 genetic markers including 16 isozymes and 68 restriction fragment length polymorphisms (RFLPs). Trait-based marker analysis (TBA) which measures changes (differences) in marker allele frequencies in selected lines was used to identify marker-linked QTLs. Eight genomic regions were identified on seven tomato chromosomes bearing genes (QTLs) with significant effects on this trait. The results confirmed our previous suggestion that salt tolerance during germination in tomato is polygenically controlled. The salt-tolerant parent contributed favorable QTL alleles on chromosomes 1, 3, 9 and 12 whereas the salt sensitive parent contributed favorable QTL alleles on chromosomes 2, 7 and 8. The identification of favorable alleles in both parents suggests the likelihood of recovering transgressive segregants in progeny derived from these parental genotypes. The results can be used for marker-assisted selection and breeding of salt-tolerant tomatoes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009668325331
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  • 6
    Electronic Resource
    Electronic Resource
    High salt tolerance potential in Lycopersicon species during germination (1986)
    Springer
    Euphytica 35 (1986), S. 575-582 
    Details
    ISSN: 1573-5060
    Keywords: Lycopersicon ; tomato ; salinity ; germination ; germplasm ; breeding ; salt-tolerance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The potential to improve seed germination responses to salinity was evaluated for 13 accessions representing six wild Lycopersicon species and 20 accessions of L. esculentum. Germination response times increased in all accessions at 100 mM NaCl. Analysis indicated that one accession of L. peruvianum (PI126435) germinated faster under high salinity than all other accessions and was closely followed by L. pennellii (LA716). The fastest germinating L. esculentum accession, PI174263, ranked third. Additional wild ecotypes exhibiting rapid germination at 100 mM NaCl were identified among L. pimpinellifolium and L. peruvianum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00021866
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  • 7
    Electronic Resource
    Electronic Resource
    Low temperature seed germination of Lycopersicon species evaluated by survival analysis (1982)
    Springer
    Euphytica 31 (1982), S. 869-883 
    Details
    ISSN: 1573-5060
    Keywords: Lycopersicon ; tomato ; low temperature germination ; survival analysis ; high altitude ecotypes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Low temperature germination responses were evaluated for 18 high altitude accessions representing five wild Lycopersicon species and 19 accessions of L. esculentum which have reputed ability to germinate in the cold. Survival analysis indicated that one accession of L. chilense germinates better at 10°C than PI 120256, the fastest-germinating L. esculentum genotype, and that PI 120256 germinates as well as PI 126435 (L. peruvianum). Additional wild ecotypes exhibiting rapid germination at 10°C were identified from L. peruvianum and L. hirsutum. These ecotypes may possess genetic potential for introgressing cold germination ability into L. esculentum cultivars.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00039227
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  • 8
    Electronic Resource
    Electronic Resource
    Phenytoin-induced connective tissue growth in the rat (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 215 (1986), S. 99-105 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Rats were treated daily for 9 days with 100, 50, or 25 mg/kg phenytoin i.p. This treatment resulted in a significant increase in the thickness of the connective tissue capsules of the liver, spleen, and pancreas, and of the subepithelial connective tissue of the mesentery but not the epicardium or visceral pleura of the lung where exposure to the drug was via the vascular route. Many areas of connective tissue growth exhibited obvious proliferation of fibroblasts and in some areas contained seemingly large numbers of macrophages and an increase in vascularity. It was demonstrated by electron microscopy that the macrophages occasionally were seen in intimate contact with the fibroblasts.Our observations clearly showed that intraperitoneal exposure of visceral connective tissues of the rat to phenytoin rapidly resulted in a dose-related proliferation of that tissue. The presence of numerous macrophages leads to the suggestion that macrophage-derived growth factor could be responsible for the increased growth.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1092150203
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  • 9
    Electronic Resource
    Electronic Resource
    Antigens on rat spermatozoa with a potential role in fertilization (1990)
    New York, NY [u.a.] : Wiley-Blackwell
    Molecular Reproduction and Development 25 (1990), S. 286-296 
    Details
    ISSN: 1040-452X
    Keywords: Monoclonal antibodies ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Eight monoclonal antibodies (McAbs), directed against antigens on rat cauda epididymal spermatozoa, were tested for their capacity to interfere with fertilization in vitro as a means of identifying molecules a potential role in sperm-egg recognition and fusion. Antigens recognized by the McAbs were visualized on live spermatozoa by indirect immunofluorescence (IIF) and characterized by immunoblotting. Five McAbs (designated 1B5, 2C4, 4B5, 5B1, and 8C4) recognized antigens specifically on the sperm acrosome and three (designated 2B1, 2D6, and 6B2) bound to the flagellum. Of the eight McAbs investigated, three (2B1, 2C4, and 6B2) were effective in blocking fertilization in vitro when added as culture supernalants to mixtures of sperm and eggs. McAb 6B2 was inhibitory due to its ability to agglutinate spermatozoa. McAbs 2B1 and 2C4 did not agglutinate capacitated spermatozoa, had no observable effect on motility, and yet blocked fertilization in a dose-dependent manner. McAb 2C4 did not give a reaction on immunoblots, but the 2B1 antigen was identified as an Mr 40 kD glycoprotein. McAb 2B1 appeared to block fertilization at the level of zona binding, whereas the effects of 2C4 were directed more against zone penetration and/or fusion with the vitellus. When sperm-egg complexes were stained with 2C4 or 2B1 McAbs and viewed by IIF, all spermatozoa that were attached to the zona showed fluorescence on the head. These results suggest that different antigens on the rat sperm head participate in different aspects of the fertilization process and that during capacitation there is either exposure of these antigens or else they migrate to their site of action from the flagellum.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1080250311
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  • 10
    Electronic Resource
    Electronic Resource
    Intracellular signal transduction mechanisms of rat epididymal spermatozoa and their relationship to motility and metabolism (1994)
    New York, NY [u.a.] : Wiley-Blackwell
    Molecular Reproduction and Development 38 (1994), S. 77-84 
    Details
    ISSN: 1040-452X
    Keywords: Cauda epididymidis ; Sperm activation ; Calcium ions ; Guanylate cyclase ; Adenylate cyclase ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The role of intracellular signal transduction mechanisms in regulating the motility and metabolism of rat spermatozoa in undiluted caudal epididymal fluid (CEF) was examined. Samples of CEF containing immotile spermatozoa were exposed to drugs and other agents that either stimulate signal transduction pathways or mimic the action of their second messengers. Under these conditions, sperm motility in 25-30 nl of CEF was stimulated by calcium ions (Ca2+), N,2′ -O-dibutyryl-guanosine 3′:5′ -cyclic monophosphate (dibutryl cGMP), cyclic adenosine 3′:5′-monophosphate (cAMP), N6,2′-O-dibutyryladenosine 3′:5′ -cyclic monophosphate (dibutyryl cAMP), 8-bromoadenosine 3′:5′ -cyclic monophosphate (8-bromo cAMP), caffeine, theophylline and bicarbonate ions (HCO3-). Other agents such as magnesium ions (Mg2+), veratridine, phospholipase C (PLC), ionophore A23187, 1,2-dioctenoyl-sn-glycerol (DAG), phorbol 12-myristate 13-acetate, phospholipase A2 (PLA2), arachidonic acid, and melittin did not significantly influence motility. In the presence of radiolabelled energy substrates, untreated (immotile) spermatozoa in samples of CEF utilised D-[U-14C]glucose and [1-14C]acetate as exogenous energy sources for oxidative metabolism. No detectable 14C-lactate was produced, and none of the drugs altered the rate of glycolytic or oxidative metabolism. The findings suggest that the motility of rat caudal epididymal spermatozoa is regulated by Ca2+ and the guanylate cyclase and adenylate cyclase pathways, but not through the PLC and PLA2 pathways. Also, their metabolism of exogenous substrate was uncoupled from the induction of motility, and their oxidative capacity exceeded the rate of flux of glucose-carbon through the glycolytic pathway. © 1994 Wiley-Liss, Inc.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1080380113
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