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  • 1
    Electronic Resource
    Electronic Resource
    Study of androgenetic performance and molecular characterisation of a set of wheat-rye addition lines (1994)
    Springer
    Theoretical and applied genetics 89 (1994), S. 982-990 
    Details
    ISSN: 1432-2242
    Keywords: RFLP analysis ; Wheat-rye addition lines ; Chromosomal rearrangements ; Anther culture ; Regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Rye chromosomes of wheat-rye addition lines were successfully identified by means of an RFLP analysis with 30 probes. Our results are in agreement with previous cytological data concerning the identity of lines F (+1R), D (+2R), C (+3R), A (+4R), E (+5R) and B (+7R). Two categories of chromosomal rearrangements have been distinguished, namely: (1) deletions: the current line D possesses a chromosome 2R deleted on its short arm and the line G a chromosome 3R deleted on its long arm; we have also noticed a deletion on the long arm of wheat chromosome 1A in line F61; and (2) evolutionary reciprocal translocations in rye relative to wheat which have been previously mentioned in the literature. The anther culture response of the different lines was studied. A significant difference between ‘FEC 28’ and the addition lines was observed for embryo production and plant regeneration. It appears that genes located on ‘S 10’ chromosome arm 3RL and on ‘FEC 28’ chromosome arm 1AL increase embryo frequency whereas gene(s) located on ‘S 10’ chromosome 5R reduce(s) it. Plant regeneration results suggest that genes increasing regeneration ability and green-plant frequency are located on ‘S 10’ chromosome 4R. The long arm of chromosome 1A seems to be involved positively in green-plant regeneration whereas chromosomes 1R and 3R limit plant regeneration.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224528
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  • 2
    Electronic Resource
    Electronic Resource
    RFLP analysis of an Aegilops ventricosa chromosome that carries a gene conferring resistance to leaf rust (Puccinia recondita) when transferred to hexaploid wheat (1995)
    Springer
    Theoretical and applied genetics 90 (1995), S. 1042-1048 
    Details
    ISSN: 1432-2242
    Keywords: RFLP analysis ; Whea ; Aegilops ventricosa ; Leaf rust resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract RFLP analysis has been used to characterise XMv, a chromosome of Aegilops ventricosa present in a disomic addition line of wheat. This chromosome is known to carry a major gene conferring resistance to leaf rust (Lr). The analysis demonstrated that XMv is translocated with respect to the standard wheat genome, and consists of a segment of the short arm of homoeologous group 2 attached to a group 6 chromosome lacking a distal part of the short arm. Lr was located to the region of XMv with homoeology to 2S by analysis of a leaf rust-susceptible deletion line that was found to lack the entire 2S segment. Confirmation and refinement of the location of Lr was obtained by analysis of a spontaneous resistant translocation in which a small part of XMv had been transferred to wheat chromosome 2A.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00222919
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  • 3
    Electronic Resource
    Electronic Resource
    Detection of QTLs for crossability in wheat using a doubled-haploid population (1998)
    Springer
    Theoretical and applied genetics 97 (1998), S. 1076-1082 
    Details
    ISSN: 1432-2242
    Keywords: Key word Crossability ; Wheat ; Rye ; Molecular markers ; QTL ; Kr genes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  An intervarietal molecular-marker map was used for the detection of genomic regions influencing crossability between wheat (Triticum aestivum L. em Thell) and rye (Secale cereale L.). Analysis of deviance and logistic marker-regression methods were conducted on data from doubled haploid lines from a cross between “Courtot” and “Chinese Spring”. A major quantitative trait locus (QTL) involved in crossability, associated with the marker Xfba367-5B, was detected on the short arm of chromosome 5B. An additional locus, Xwg583-5B, was indicated on the long arm of chromosome 5B. This minor QTL might correspond to Kr1 which was presumed to be the major gene controlling crossability. Another locus of the genome, Xtam51-7A on chromosome 7A, was significantly associated with this trait. Alleles of “non-crossability” were contributed by the non-crossable cultivar “Courtot”. The three-marker model explains 65% of the difference in crossability between the two parents. The present results are discussed in relation to those previously carried out to locate the Kr genes by using the telocentric mapping technique.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050994
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  • 4
    Electronic Resource
    Electronic Resource
    Molecular markers linked to genes affecting plant height in wheat using a doubled-haploid population (1998)
    Springer
    Theoretical and applied genetics 96 (1998), S. 933-940 
    Details
    ISSN: 1432-2242
    Keywords: Key words Plant height ; Molecular markers ; QTL ; Wheat ; Doubled-haploid lines
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Plant height in wheat (Triticum aestivum L. em Thell) is known to be under polygenic control. Crosses involving genes Rht-B1 and Rht-D1, located on chromosomes 4BS and 4DS, respectively, have shown that these genes have major effects. Two RFLP loci were found to be linked to these two genes (Xfba1-4B with Rht-B1 and Xfba211-4D with Rht-D1) by genotyping a population of F1-derived doubled-haploid lines [‘Courtot’ (Rht-B1b+Rht-D1b)בChinese Spring’]. Using a well-covered molecular marker map, we detected three additional regions and one interaction influencing plant height. These regions, located on chromosome arms 4BS (near the locus Xglk556-4B), 7AL (near the locus Xglk478-7A) and 7BL (near the locus XksuD2-7B) explained between 5% and 20% of the variability for this trait in this cross. The influence of 2 loci from chromosome 4B (Xfba1-4B and Xglk556-4B) suggests that there could be a duplication of Rht-B1 on this chromosome originating from Cv ‘Courtot’. Moreover, an interaction effect between loci from chromosome arms 1AS (near the locus Xfba393-1A) and 1BL (near the locus Xcdo1188-1B) was comparable to or even higher than those of the Rht-B1b and Rht-D1b alleles. A model including the main effects of the loci from chromosomes 4B and 4D (Xfba1-4B, Xglk556-4B and Xfba211-4D) and the interaction effect between Xfba393-1A and Xcdo1188-1B is proposed, which explains about 50% of the variation in plant height. The present results are discussed in relation to those obtained using nullisomic or substitution lines.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050823
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  • 5
    Electronic Resource
    Electronic Resource
    Location and mapping of the powdery mildew resistance gene MlRE and detection of a resistance QTL by bulked segregant analysis (BSA) with microsatellites in wheat (2000)
    Springer
    Theoretical and applied genetics 100 (2000), S. 1217-1224 
    Details
    ISSN: 1432-2242
    Keywords: Key words Triticum aestivum ; Blumeria graminis f. sp. tritici ; QTL mapping ; Molecular markers ; Disease resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Powdery mildew (Blumeria graminis f. sp. tritici) is one of the most damaging diseases of wheat (Triticum aestivum). The objective of this study was to locate and map a recently identified powdery mildew resistance gene, MlRE, carried by the resistant line RE714 using microsatellites uniformly distributed among the whole genome together with a bulked segregant analysis (BSA). The bulks consisted of individuals with an extreme phenotype taken from a population of 140 F3 families issued from the cross between RE714 (resistant) and Hardi (susceptible). The population had been tested with three powdery mildew isolates at the seedling stage. Qualitative interpretation of the resistance tests located the MlRE gene on the distal part of the long arm of chromosome 6A. A subsequent quantitative interpretation of the resistance permitted us to detect another resistance factor on a linkage group assigned to chromosome 5D, which was constructed with microsatellites for which a polymorphism of intensity between bulks was observed. This quantitative trait locus (QTL) explained 16.8– 25.34% of the total variation. An interaction between both the resistant factor (MlRE and the QTL) was found for only one of the isolates tested. This study shows the advantage of making a quantitative interpretation of resistant tests and that the use of microsatellites combined with BSA is a powerful strategy to locate resistance genes in wheat.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220051427
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