Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    Enrichment of heterokaryocytes between mesophyll and epidermis protoplasts by density gradient centrifugation after electric fusion (1987)
    Springer
    Theoretical and applied genetics 75 (1987), S. 26-29 
    Details
    ISSN: 1432-2242
    Keywords: Density gradient centrifugation ; Electric fusion ; Epidermis protoplasts ; Heterokaryocyte enrichment ; Mesophyll protoplasts ; Nicotiana
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Mesophyll protoplasts from Nicotiana glauca were fused with epidermal protoplasts from N. langsdorffii by an electric pulse. After the fusion products were centrifuged on stepwise density gradient centrifugation using Percoll and sea water, somatic hybrids were observed at 70%–80% in the fraction recovered from the intermediate specific gravity fraction between epidermis and mesophyll protoplasts. From offsprings of these somatic hybrids, teratomatous plants were regenerated. Since the difference of specific gravity between mesophyll and epidermis protoplasts is inherent, this procedure can be essentially applied to obtain somatic hybrids between any combination of plants. The significance of this study is discussed in relation to obtaining somatic hybrids between plant materials without any appropriate genetic markers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00249137
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Forensic analysis of triazolam in human tissues using capillary gas chromatography (1991)
    Springer
    International journal of legal medicine 104 (1991), S. 67-69 
    Details
    ISSN: 1437-1596
    Keywords: Triazolam ; Estazolam ; Capillary GC/NPD ; Human tissue ; Forensic toxicology ; Triazolam ; Estazolam ; Kapillar-Gaschromatographie/NPD ; Menschliches Gewebe ; Forensische Toxikologie
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Law
    Description / Table of Contents: Zusammenfassung Eine zuverlässige und empfindliche Methode wurde entwickelt, um die Konzentrationen des hypnotischen Medikaments Triazolam in menschlichen Geweben, einschließlich fauler Gewebe bestimmen zu können. Die Methode besteht aus einer dreistufigen Flüssig-Flüssig-Extraktion, einem Clean up an Kieselgel und Gaschromatographie mit einem Stickstoff-PhosphorDetektor und einer Kapillarsäule. Als interner Standard wurde Estazolam benutzt. Die Eichkurve war im Konzentrationsbereich zwischen 1 ng/g und 1 gg/g linear, und die untere Nachweisgrenze betrug 0.5 ng/g. Es wurde eine forensische Untersuchung über die toxikologischen Effekte von Triazolam an faulem Gewebe durchgeführt.
    Notes: Summary A reliable and sensitive method has been developed to assess the concentrations of the hypnotic drug triazolam in human tissues, including putrefied tissues. The method involves a 3-step solvent extraction, cleanup on a silica gel column and gas chromatography using a nitrogen phosphorus detector and a capillary column. Estazolam was used as an internal standard. The calibration curve was linear over the concentration range 1 ng/g1 gm/g and the lower limit of detection was 0.5 ng/g. A forensic study was performed on the toxicological effects of triazolam using putrefied tissues.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01626033
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    A case of drowning linked to ingested sulfides — a report with animal experiments (1996)
    Springer
    International journal of legal medicine 109 (1996), S. 42-44 
    Details
    ISSN: 1437-1596
    Keywords: Toxicology ; Drowning ; Sulfides ; Human tissue ; Distribution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Law
    Notes: Abstract An adult male was found dead beneath a pool of sewage in the pump room of a fish market. Autopsy revealed the cause of death to be suffocation after aspirating sewage into the respiratory tract. Since hydrogen sulfide gas was detected in the atmosphere at the scene of the accident, gas poisoning was suspected and toxicological analysis of sulfides in body tissues was performed. The concentrations of sulfides in the blood, lung and kidney were 0.95 μmol/ml, 0.22 and 0.38 μmol/g, respectively. These values were remarkably higher than those in previously reported cases involving exposure to hydrogen sulfide gas. Therefore, oral intake of sulfides was assumed and the distribution of sulfides in tissues following oral administration of sodium sulfide solution was examined by means of animal experiments using rats. The concentration of sulfides in the blood from rats following oral intake was much higher than that seen following gas exposure. Based on these results, we concluded that the victim had been exposed to hydrogen sulfide gas and had then collapse into a pool of sewage containing sulfides. The sulfides which were distributed throughout the body tissues had mainly issued from the alimentary tract prior to death by drowning.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01369601
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Organization of heterogeneous mitochondrial DNA molecules in mitochondrial nuclei of cultured tobacco cells (1993)
    Springer
    Protoplasma 175 (1993), S. 112-120 
    Details
    ISSN: 1615-6102
    Keywords: Cell culture ; DNA content ; Mitochondrial DNA ; Mitochondrial genome ; Nicotiana
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The molecular size of mitochondrial DNA (mtDNA) molecules and the number of copies of mtDNA per mitochondrion were evaluated from cultured cells of the tobacco BY-2 line derived fromNicotiana tabacum L. cv. Bright Yellow-2. To determine the DNA content per mitochondrion, protoplasts of cultured cells were stained with 4′,6-diamidino-2-phenylindole (DAPI), and the intensity of the fluorescence emitted from the mitochondrial nuclei (mt-nuclei) was measured with a video-intensified photon counting microscope system (VIM system). Each mitochondrion except for those undergoing a division contained one mt-nucleus. The most frequently measured size of the DNA in the mitochondria was between 120 and 200 kilobase pairs (kbp) throughout the course of culture of the tobacco cells. Mitochondria containing more than 200 kbp of DNA increased significantly in number 24 h after transfer of the cells into fresh medium but their number fell as the culture continued. Because division of mitochondria began soon after transfer of the cells into fresh medium and continued for 3 days, the change of the DNA content per mitochondrion during the culture must correspond to DNA synthesis of mitochondria in the course of mitochondrial division. By contrast, the analyses of products of digestion by restriction endonucleases indicated that the genome size of the mtDNA was at least 270 kbp. Electron microscopy revealed that mtDNAs were circular molecules and their length ranged from 1 to 35 μm, and 60% of them ranged from 7 to 11 μrn. These results indicate that the mitochondrial genome in tobacco cells consists of multiple species of mtDNA molecules, and mitochondria do not contain all the mtDNA species. Therefore, mitochondria are heterogeneous in mtDNA composition.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01385008
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...