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  • Nodulation  (2)
  • Organic Chemistry  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Nodulation of soybean byRhizobium japonicum mutants with altered capsule synthesis (1982)
    Springer
    Planta 154 (1982), S. 100-109 
    Details
    ISSN: 1432-2048
    Keywords: Mutant (Rhizobium) ; Nodulation ; Capsule (bacterial) ; Lectin ; Polysaccharide ; Rhizobium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Spontaneous mutants with altered capsule synthesis were isolated from a marked strain of the symbiont,Rhizobium japonicum. Differential centrifugation was used to enrich serially for mutants incapable of forming capsules. The desired mutants were detected by altered colony morphology and altered ability to bind host plant lectin. Three mutants failed to form detectable capsules at any growth phase when cultured in vitro or in association with the host (soybean,Glycine max (L.) Merr.) roots. These mutants were all capable of nodulating and attaching to soybean roots, indicating that the presence of a capsule physically surrounding the bacterium is not required for attachment or for infection and nodulation. Nodulation by several of the mutants was linearly proportional to the amount of acidic exopolysaccharide that they released into the culture medium during the exponential growth phase, indicating that such polysaccharide synthesis is important and perhaps required for nodulation. Two of the mutants appeared to synthesize normal lectin-binding capsules when cultured in association with host roots, but not when cultured in vitro. Nodulation by these mutants appeared to depend on how rapidly after inoculation they synthesized capsular polysaccharide.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00387901
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  • 2
    Electronic Resource
    Electronic Resource
    Spot inoculation of soybean roots withRhizobium japonicum (1983)
    Springer
    Protoplasma 115 (1983), S. 122-128 
    Details
    ISSN: 1615-6102
    Keywords: Inoculation ; Nodulation ; Rhizobium japonicum ; Soybean ; Symbiosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A method has been developed to inoculate soybean roots in the zone of susceptible root cells with nanoliter droplets containing the symbiont,Rhizobium japonicum. The position of inoculation is marked by means of ion exchange beads which adhere to the root surface. InoculatedR. japonicum cells from a nanoliter droplet spread over an area of approximately 35 host epidermal cells and have a high probability (∼ 80%) of inducing nodule formation at exactly the point of inoculation. The spot inoculation method is highly efficient in terms of the number of bacteria required to achieve a given probability of nodule formation. The method greatly facilitates ultrastructural analysis of the infection process and is useful for certain physiological studies of infection.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01279804
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Tieffarbige Systeme mit Oxalsäurestruktur  -  eine Strukturkorrektur (1991)
    New York, NY : Wiley-Blackwell
    Journal für Praktische Chemie/Chemiker-Zeitung 333 (1991), S. 555-559 
    Details
    ISSN: 0021-8383
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Deep Coloured Systems with Oxalic Acid Substructure-a Structural CorrectionBasing on a recent spectroscopic investigation the structure of earlier described deeply coloured systems derived from oxalic acid imidochlorides and thioacetamide has to be revised. Due to the poor solubility of these systems their spectroscopic characterization led to the misinterpretation as tetramethin-bridged thiazolines 1. By means of 1H-NOE-Difference-Spectroscopy of newly synthesized derivatives with lipophilic substituents a imidazolin-thion substructure 4 has been found. Such an arrangement of the heteroatoms clearly reflects a 1,3-rearrangement with participation of the oxalic acid system.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/prac.19913330406
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  • 4
    Electronic Resource
    Electronic Resource
    Über die Reaktion der 3-Acyl-1,5-dibrom-pentane mit Ammoniak (1951)
    New York, NY : Wiley-Blackwell
    Helvetica Chimica Acta 34 (1951), S. 736-744 
    Details
    ISSN: 0018-019X
    Keywords: Chemistry ; Organic Chemistry
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: 3-Acyl-1,5-dibrom-pentane I und IX geben durch Erhitzen mit methanolischem Ammoniak auf 110° sauerstoffreie bicyclische, ungesättigte Basen, welchen auf Grund ihrer physikalischen und chemischen Eigenschaften die Konstitution VI bzw. X zugeschrieben werden konnte.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/hlca.19510340237
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    Paper (German National Licenses)
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