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  • 1990-1994  (2)
  • P transformation  (1)
  • Rat  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Nutrient cycling in agroecosystems 29 (1991), S. 173-185 
    ISSN: 1573-0867
    Keywords: Soil ; fused phosphate ; incubation ; dissolution ; P fractions ; P level ; P transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The dissolution of fused calcium phosphate (FP) and transformation of P were monitored in a silt loam, sandy loam, sandy clay and clay soil (pH in water = 5.3 − 5.8) incubated with 0, 108 or 324 mg P, as FP kg−1 soil dry wt, for 10, 30 or 60 d. Soluble P was fractionated by sequential extraction using a chloride anion exchanger (P-resin) and solutions of NaHCO3 (PtCO3), NaOH (PtOH) and HCl (P-HCl). Inorganic P (PiCO3, PiOH) and organic P (PoCO3, PoOH) were also determined. The FP treatments significantly (P = 5%) increased both P-resin and P-HCl levels. Over time, the differences between P-resin or P-HCl levels and the controls declined, except in the silt loam soil in which P-resin levels nearly doubled. PtCO3 and PtOH levels did not markedly respond to FP applications. As incubation proceeded PtOH levels significantly (P = 5%) increased both as PiOH and PoOH. This PtOH buildup was accompanied by small declines in PtCO3 levels, with the declines being greater in the PoCO3 than in PiCO3 fraction. P-resin, PiOH and PoOH levels were negatively correlated with those of p-HCl (P = 5%, n = 36). P-resin levels were also negatively correlated with those of PoCO3. Of the small and large FP doses, 12–45% and 51–56%, respectively, had yet to react by the end of the experiment.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-1106
    Keywords: Dorsal root ganglion ; Nucleus gracilis ; Sciatic nerve ; Nerve injury ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Rat lumbar dorsal root ganglion neurones projecting to the nucleus gracilis in the brainstem were retrogradely labelled with Fluoro-Gold and analysed immunocytochemically for their expression of substance P-, calcitonin gene-related peptide-, galanin-, galanin message-associated peptide-, neuropeptide Y-, nitric oxide synthase- and carbonic anhydrase-like immunoreactivity as well as affinity to Griffonia (bandeiraea) simplicifolia lectin I — isolectin B4, RT97 and to choleragenoid. The analysis was made both in uninjured rats and in rats which had been subjected to unilateral sciatic nerve transection and partial resection 3 weeks earlier. The data showed that 6% of the L4 and L5 lumbar dorsal root ganglion cells that projected to the nucleus gracilis showed substance P-like immunoreactivity. Following nerve injury, none of the nucleus gracilis-projecting dorsal root ganglion cells showed substance P-like immunoreactivity. Nineteen per cent of the investigated cell population showed calcitonin gene-related peptide-like immunoreactivity in uninjured rats, but no nucleus gracilisprojecting calcitonin gene-related peptide-positive cells were found after nerve injury. Galanin- and galanin message-associated peptide-like immunoreactivity were found in 2% and 3%, respectively, of the Fluoro-Gold-labelled cell population normally and in 22% and 14%, respectively, after injury. No neuropeptide Y-positive cells were found in the Fluoro-Gold-labelled cell population normally, but after nerve injury, 96% of this population became neuropeptide Y-positive. Nitric oxide synthaselike immunoreactivity was found in 2% of the Fluoro-Gold-labelled cells normally and in 10% after injury. Two per cent of the Fluoro-Gold-labelled cells in the normal cases were stained by Griffonia (bandeiraea) simplicifolia lectin I — isolectin B4. After injury, however, no such double labelling was found. Thirty-four per cent of the Fluoro-Gold-labelled cell population was carbonic anhydrase positive normally, and 42% after injury. Seventy-five per cent of the Fluoro-Gold-labelled cells showed RT97 immunoreactivity normally and 12% after injury. Choleragenoid-like immunoreactivity was found in 99% of the Fluoro-Gold-labelled dorsal root ganglion cells normally and 81% after injury. Immunohistochemical visualisation of choleragenoid transganglionically transported from the injured sciatic nerve combined with neuropeptide Y immunocytochemistry showed that primary afferent fibres and terminals in the nucleus gracilis contain neuropeptide Y following peripheral nerve transection. Taken together, the results indicate that peripherally axotomised nucleus gracilis-projecting neurones undergo marked alterations in their cytochemical characteristics, which may be significant for the structural and functional plasticity of this system after injury.
    Type of Medium: Electronic Resource
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