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  • 1
    Electronic Resource
    Electronic Resource
    Genomic in situ hybridization to identify alien chromosomes and chromosome segments in wheat (1992)
    Springer
    Theoretical and applied genetics 84 (1992), S. 778-786 
    Details
    ISSN: 1432-2242
    Keywords: Genomic probing ; In situ hybridization ; Interphase cytogenetics ; Physical mapping ; Triticum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Genomic in situ hybridization was used to identify alien chromatin in chromosome spreads of wheat, Triticum aestivum L., lines incorporating chromosomes from Leymus multicaulis (Kar. and Kir.) Tzvelev and Thinopyrum bessarabicum (Savul. and Rayss) Löve, and chromosome arms from Hordeum chilense Roem. and Schult, H. vulgare L. and Secale cereale L. Total genomic DNA from the introgressed alien species was used as a probe, together with excess amounts of unlabelled blocking DNA from wheat, for DNA:DNA in-situ hybridization. The method labelled the alien chromatin yellow-green, while the wheat chromosomes showed only the orange-red fluorescence of the DNA counterstain. Nuclei were screened from seedling root-tips (including those from half-grains) and anther wall tissue. The genomic probing method identified alien chromosomes and chromosome arms and allowed counting in nuclei at all stages of the cell cycle, so complete metaphases were not needed. At prophase or interphase, two labelled domains were visible in most nuclei from disomic lines, while only one labelled domain was visible in monosomic lines. At metaphase, direct visualization of the morphology of the alien chromosome or chromosome segment was possible and allowed identification of the relationship of the alien chromatin to the wheat chromosomes. The genomic in-situ hybridization method is fast, sensitive, accurate and informative. Hence it is likely to be of great value for both cytogenetic analysis and in plant breeding programmes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00227384
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  • 2
    Electronic Resource
    Electronic Resource
    Polymer treatment in the flowing afterglow of an oxygen microwave discharge: Active species profile concentrations and kinetics of the functionalization (1995)
    Springer
    Plasma chemistry and plasma processing 15 (1995), S. 173-198 
    Details
    ISSN: 1572-8986
    Keywords: Singlet molecular oxygen ; atomic oxygen ; flowing afterglow ; downstream reactor ; microwave discharge ; PP ; PF ; PC ; hexatriacontane ; octadecyloctadecanoate ; functionalization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Technology
    Notes: Abstract The surface treatment of different polymers and their corresponding model surfaces in the flowing afterglow of an oxygen microwave plasma is investigated. The concentration profiles of tire long-lived species issued from the plasma are measured and calculated in the downstream area The influence of atomic and singlet molecular oxygen in the behavior of different polymers is investigated. It appears that the evolution of the surface energy can be explained by an initiation of the functionalization by the oxygen atoms impinging upon the surface followed by reaction of the radicals formed with molecular oxygen. The concentration of functions at the sureface is limited due to their destruction by reaction with oxygen atoms. Furthermore, the functionalization level is higher in the /lowing afterglow than in the plasma, without any significant degradation of the polymer surface. Therefore, the treatment in the flowing afterglow is more efficient to increase suface energy in particular, for polymers which undergo high backbone chain scission.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01459695
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    Paper (German National Licenses)
    Fulltext
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