ISSN:
1432-1424
Schlagwort(e):
Retina
;
Glial cell
;
Potassium channel
;
Tetraethylammonium
;
Patch clamp
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Biologie
,
Chemie und Pharmazie
Notizen:
Abstract Ion channels present in isolated glial (Müller) cells from the retina of the turtle (Pseudemys scripta elegans) were studied with the patch clamp technique. The predominant conductance in these cells was due to an inward rectifying potassium current. The whole-cell conductance of the inward rectifier was 20.2±1.9 nS (n = 7 cells) in a standard extracellular saline solution (3 mm extracellular potassium). This conductance was dependent on the extracellular potassium concentration, with a 2.88-fold change in conductance per tenfold shift in concentration. The relative permeability sequence to potassium of the inward rectifier was found to be: potassium (1.0) 〉 rubidium (0.7) 〉 ammonium (0.2) 〉 lithium (0.1) = sodium (0.1), which corresponded to the Eisenman sequence IV or V for a strongfield-strength potassium binding site on the channel. The single channel conductance measured in cell-attached patches with potassium chloride (150 mm) in the pipette was 68.5 ± 6.0 pS (n = 3 patches). The inward rectifier current was not blocked by extracellular tetraethylammonium (TEA+, 20 mm), but was blocked by extracellular barium (5 mm) or cesium (5 mm). The TEA+ insensitivity of the inward rectifier potassium channel in Müller cells is unusual, given that this type of channel in most excitable cells is sensitive to micromolar concentrations of this compound, and may be a characteristic of inward rectifier potassium channels that are primarily involved with extracellular potassium regulation.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF00235133
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