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  • 1
    Electronic Resource
    Electronic Resource
    Genetic variation in the subunits of globulin-1 storage protein of French bean (1981)
    Springer
    Theoretical and applied genetics 59 (1981), S. 83-88 
    Details
    ISSN: 1432-2242
    Keywords: Phaseolus vulgaris ; Storage proteins ; Electrophoresis ; Genetic variation ; Banding types
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Charge and molecular weight heterogeneity of globulin-1 (G1) polypeptides of the bean, Phaseolus vulgaris L., were revealed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). Different bean cultivars were classified into three groups: ‘Tendergreen’, ‘Sanilac’, and ‘Contender’ on the basis of their protein subunit composition. Nine distinct major bands: α51,α49, α48.5,β48T, β48S, β47, γ45.5, γ45S, and γ45C, and two minor bands: γ46T and γ46S were found to account for the three profiles seen on one-dimensional SDS-PAGE. Two-dimensional analysis revealed these eleven protein bands to be composed of a minimum of fourteen distinct protein subunits. The ‘Tendergreen’ and ‘Sanilac’ types differ in their G1 polypeptide composition. The protein patterns of the ‘Contender’ types are intermediate, containing many protein subunits found in the patterns of the ‘Tendergreen’ and ‘Sanilac’ types suggesting a genetic and evolutionary relationship.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00285895
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  • 2
    Electronic Resource
    Electronic Resource
    Linkage relationships between genes controlling seed proteins in French bean (1981)
    Springer
    Theoretical and applied genetics 60 (1981), S. 251-259 
    Details
    ISSN: 1432-2242
    Keywords: Phaseolus vulgaris ; Phaseolin ; Seed proteins ; Electrophoresis ; Linkage
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The inheritance of phaseolin and globulin-2 (G2)/albumin polypeptides was investigated in crosses involving varieties which exhibited the three electrophoretic banding patterns of phaseolin found in French bean. ‘Total’ seed protein extracts of single seeds of the F1 and F2 generations from the crosses: ‘Sanilac’ × ‘Contender’, ‘BBL 240’ × ‘Contender’, and ‘Sanilac’ × ‘BBL 240’ were analyzed by two-dimensional electrophoresis. Segregation of the genes controlling phaseolin and G2/albumin polypeptides, and those controlling a further five groups of seed proteins (A, B, D, E, and F) were observed. No recombinant electrophoretic phenotypes were seen for phaseolin or G2/albumin polypeptides suggesting that the genes controlling each of these groups of polypeptides are closely linked and segregate like single Mendelian genes. The phaseolin genes and G2/albumin genes were not linked to each other. The group of genes controlling phaseolin polypeptides were linked to those controlling group B proteins, and those controlling G2/albumin polypeptides were linked to those controlling group F proteins.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02342545
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  • 3
    Electronic Resource
    Electronic Resource
    Bean lectins (1982)
    Springer
    Theoretical and applied genetics 62 (1982), S. 263-271 
    Details
    ISSN: 1432-2242
    Keywords: Phaseolus vulgaris ; Seed protein ; Lectins ; Electrophoresis ; Agglutination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Single seeds of over 100 bean cultivars were analyzed by two-dimensional electrophoresis. The cultivars could be classified into eight groups by virtue of their G2/albumin electrophoretic patterns: TG2, SG2, VG2, PrG2, BG2, MG2, PG2, and PiG2, The polypeptide compositions of these types were largely inter-related having particular polypeptides in common. It was possible to correlate the G2/albumin patterns with agglutinating activity of cow and rabbit blood cells as measured by the agglutination ratio (minimum concentration of extract required to agglutinate cow blood cells: minimum concentration of extract required to agglutinate rabbit blood cells). The active lectin polypeptides were identified by extracting lectins from agglutinated erythrocytes and by comparing the qualitative similarities and differences of the G2/albumin patterns and their agglutination activities. A reference catalogue of over 100 bean cultivars giving their phaseolin and G2/albumin electrophoretic patterns, and agglutination ratios is presented.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00276249
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  • 4
    Electronic Resource
    Electronic Resource
    Bean lectins (1982)
    Springer
    Theoretical and applied genetics 62 (1982), S. 361-367 
    Details
    ISSN: 1432-2242
    Keywords: Phaseolus vulgaris ; Lectins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The relationship between the polypeptide composition and the agglutination behaviour of the lectin-containing G2/albumin protein groups has allowed the identification of the active lectin polypeptides in different cultivars of Phaseolus vulgaris (Brown et al. accompanying paper). These results were used to ascertain the particular G2/albumin group contained in the various lectin sources used previously for the purification of lectin proteins. Many studies were found to have included lectin sources which contained the same G2/albumin pattern (TG2) and this common denominator has permitted the direct comparison of the properties reported for these purified lectins. Thus, much of the extensive literature on bean lectins is concurred.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00275105
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  • 5
    Electronic Resource
    Electronic Resource
    Bean lectins (1985)
    Springer
    Theoretical and applied genetics 70 (1985), S. 22-31 
    Details
    ISSN: 1432-2242
    Keywords: Phaseolus vulgaris ; Lectin ; Phaseolin ; Quantitative variation ; Immunoelectrophoresis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Seeds of forty bean cultivars having different lectin types based on two-dimensional isoelectric focusing-sodium dodecyl sulfate polyacrylamide gel electrophoresis (IEF-SDS/PAGE) were analyzed for quantities of lectin, phaseolin and total protein. Significant differences were found among groups of cultivars with different lectin types for the quantity of lectin and phaseolin. Cultivars with more complex lectin types based on IEF-SDS/PAGE tended to have higher quantities of lectin and lower quantities of phaseolin than cultivars with simple lectin types. An association between lectin type and the quantity of lectin and phaseolin was found also in the seeds of F2 plants that segregated in a Mendelian fashion for two lectin types. Seeds from plants with the complex lectin type had more lectin and less phaseolin than seeds from plants with the simple lectin type. Therefore, the genes controlling qualitative lectin variation also may influence the quantitative variation of lectin and phaseolin. The results of this study are related to other studies on the quantitative variation for seed proteins and to the possible molecular basis for variation in the quantity of lectins in beans.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00264478
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  • 6
    Electronic Resource
    Electronic Resource
    Bean lectins IV: genetic variation in the non-denatured structure of lectins from different Phaseolus vulgaris L. cultivars (1984)
    Springer
    Theoretical and applied genetics 67 (1984), S. 547-552 
    Details
    ISSN: 1432-2242
    Keywords: Phaseolus vulgaris ; Lectins ; Albumin ; Globulin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Variation in the native conformation of bean lectins was examined using electrophoresis of non-denatured total protein extracts and purified albumin and globulin lectin. The observed variation was related to the genetic variation reported previously for lectin polypeptide composition as revealed by two-dimensional isoelectricfocusing-sodium dodecyl sulfate polyacrylamide gel electrophoresis (IEF-SDS/PAGE). When eleven cultivars with different IEF-SDS/PAGE lectin polypeptide compositions were compared, eight had unique non-denatured lectin patterns and three had identical patterns. For some cultivars differences in non-denatured lectin patterns were observed between the purified albumin and globulin lectin preparations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00264902
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  • 7
    Electronic Resource
    Electronic Resource
    The effect of hydrogen chloride on the corrosion of an FeCrAlY alloy in simulated coal gasifier atmospheres (1989)
    Weinheim [u.a.] : Wiley-Blackwell
    Materials and Corrosion/Werkstoffe und Korrosion 40 (1989), S. 175-181 
    Details
    ISSN: 0947-5117
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Description / Table of Contents: Einfluß von Chlorwasserstoff auf die Korrosion einer FeCrAlY-Legierung in einer simulierten KohlevergasungsatmosphäreEie Chrom-Aluminium-Yttrium-Stahl wurde einer simulierten Kohlevergasungsatmosphäre ausgesetzt die 1000 ppm bzw. 2200 ppm Chlorwasserstoff enthielt und deren Temperatur 450 °C betrug. Durch Erhöhen des Chlorwasserstoffgehalts werden die Reaktionsgeschwindigkeit erhöht und die Mikrostruktur sowie die Zusammensetzung des Korrosionsprodukts beträchtlich verändert. Für diese Ergebnisse werden versuchsweise Mechanismen postuliert, nach denen Metallchloride über die Gasphase transportiert werden.
    Notes: An iron chromium aluminium yttrium steel was exposed to a simulated coal gasifier atmosphere containing 1000 ppm and 2200 ppm hydrogen chloride at 450 °C. Increasing hydrogen chloride content was found to accelerate reaction rates, and significantly alter the microstructure and composition of the corrosion product. Tentative explanations for these results, involving vapour phase transport of metal chlorides are proposed.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/maco.19890400312
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  • 8
    Electronic Resource
    Electronic Resource
    Surface modification of extended chain polyethylene fibres to improve adhesion to epoxy and unsaturated polyester resins (1991)
    Chichester [u.a.] : Wiley-Blackwell
    Surface and Interface Analysis 17 (1991), S. 143-150 
    Details
    ISSN: 0142-2421
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Physics
    Notes: Extended chain polyethylene fibres have been treated in ammonia and oxygen lo-pressure gas discharges (plasmas) in order to enhance adhesion to epoxy and unsaturated polyester resins, respectively, and thus significantly improve fibre/resin interfacial properties in fibre-reinforced polymer composites. Ammonia plasma treatment results in the incorporation of amine functional groups onto the fibre suface. The treated fibre surface has been analysed using XPS and spectrophotometric techniques. Extended chain polyethylene/epoxy composites made from ammonia, plasma-treated fibres show a marked increase in interlaminar shear strength over composites made from untreated, corona-treated or oxygen plasma-treated fibres. The increase in fibre/resin adhesion after ammonia plasma treatment is confirmed by SEM observations of fracture surfaces, which show clean interfacial fracture surfaces in composites made from treated fibres. Fibres modified by oxygen plasma treatment contain a significant concentration of carbon-oxygen functionalities, which contribute to the polarity of the surface and hence increase wet-out by unsaturated polyester resins. The concentration and nature of carbon-oxygen species on the fibre surface have been determined by XPS. Pull-out tests on multifilament yarns embedded in a polyester resin confirm the high fibre/matrix adhesion achieved with the oxygen plasma-treated fibres compared to corona-treated or untreated fibres. Tensile properties of the fibres are reduced significantly after prolonged treatment in an oxygen plasma, while in an ammonia plasma the fibre strength is unaffected.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/sia.740170305
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  • 9
    Electronic Resource
    Electronic Resource
    Letter to the editor (1976)
    New York, NY [u.a.] : Wiley-Blackwell
    Fire and Materials 1 (1976), S. 83-84 
    Details
    ISSN: 0308-0501
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Architecture, Civil Engineering, Surveying , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/fam.810010209
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  • 10
    Electronic Resource
    Electronic Resource
    Fire-hazard assessment of extended-chain polyethylene and aramid composites by cone calorimetry (1994)
    New York, NY [u.a.] : Wiley-Blackwell
    Fire and Materials 18 (1994), S. 167-172 
    Details
    ISSN: 0308-0501
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Architecture, Civil Engineering, Surveying , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: A cone calorimeter is used to determine the fire performance of polymer composite materials containing combustible reinforcing fibres in addition to combustible matrix resins. Extended-chain polyethylene and aramid fibre-reinforced composites containing epoxy, vinylester and phenolic matrix resins are examined at various cone irradiances. Values for time to ignition, rate of heat release, effective heat of combustion, smoke density and evolved carbon monoxide and carbon dioxide are reported for the reinforcements, matrix resins and composites. The reinforcements have a significant effect on the fire-hazard properties of the composite materials. For the epoxy and vinylester composites, times to ignition reflect those of the component of higher ignitability. This was not the case for the aramid-reinforced phenolic composite, in which the resin surface layer hinders combustion of the fabric reinforcement. Resin and reinforcement contributions to the composite rate of heat release behaviour as a function of time are generally discernible.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/fam.810180304
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