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  • Plasmid-like DNA  (2)
  • Expansive z-förmige Laminoplastik  (1)
  • 1
    ISSN: 1432-0983
    Keywords: Key words Mitochondrial DNA ; Plasmid-like DNA ; Temperature dependency ; Rice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The mitochondria of rice contain four kinds of circular plasmid-like DNAs, namely, B1, B2, B3 and B4, in addition to the main mitochondrial genomic DNAs. In order to examine the genetic stability of mitochondrial plasmid-like DNAs, changes in the amounts of plasmid-like DNAs and main mitochondrial genomic DNAs were analyzed in calli that had been cultured at various temperatures. The observed effect of temperature on the levels of plasmid-like DNAs was larger than that on the main mitochondrial genomic DNAs. A significant reduction in the copy number of plasmid-like DNAs was detected in calli cultured at 35 °C, as compared to 20 °C, 25 °C and 30 °C. The effect of temperature on DNA synthesis in isolated mitochondria was also analyzed. Synthesis of the main mitochondrial genomic DNAs occurred at all the temperatures examined, whereas synthesis of plasmid-like DNAs occurred only over a limited range of temperatures. The results of both in vivo and in vitro analyses suggest that plasmid-like DNAs are less stably maintained than the main mitochondrial genomic DNAs, which is consistent with the notion that the transmission of mitochondrial plasmid-like DNAs from one generation to the next may be unstable under unusual conditions.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-2242
    Keywords: Mitochondrial DNA ; Plasmid-like DNA ; Nuclear homologue ; Inter-organellar sequence transfer ; Rice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The chromosomal locations of restriction fragments of nuclear DNA that were homologous to four mitochondrial plasmid-like DNAs, namely, B1, B2, B3 and B4, were analyzed by restriction fragment length polymorphism (RFLP) analysis in cultivated rice. Nine kinds of fragments homologous to plasmidlike DNAs were analyzed for their segregation in three different F2 populations derived from intercrosses between rice subspecies; these were found to be localized in three chromosomal regions: three, one and five kinds of nuclear homologues were situated on chromosomes 1,3 and 8, respectively. Nuclear homologues on a given chromosome were tightly linked even though they were homologous to different plasmid-like DNAs. The loci of nuclear homologues found commonly in two or three cultivars were found to be highly conserved, a result that is consistent with their stable transmission. These results and those of the Southern analysis suggest the independent integration of these sequences during the varietal differentiation of rice. The concentration of loci for nuclear homologues on chromosomes 1 and 8 suggests that these sequences were integrated non-randomly into these chromosomal regions.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Der Orthopäde 25 (1996), S. 533-541 
    ISSN: 1433-0431
    Keywords: Schlüsselwörter Zervikale komprimierende Myelopathie ; Dorsale Dekompression ; Laminoplastikverfahren ; Extensive simultane dekomprimierende Laminektomie ; Expansive z-förmige Laminoplastik ; Expansive „Open-door-Laminoplastik“ ; Key words Posterior approach ; Cervical spine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary The posterior approach to the cervical spine was the only method of access to the spinal canal until the anterior approach was developed by Robinson and Smith, and Cloward. With the accumulation of experience in posterior decompression for cervical spondylotic myelopathy (CSM), successful laminectomy was guaranteed only when lordotic alignment of the cervical spine, wide and extensive laminectomy for adequate posterior shift of the spinal cord, and stability of the spine were ensured after surgery. The thick scar formation occasionally seen subsequent to postlaminectomy hematoma could lead to an unfavorable outcome. The insertion of surgical instruments, such as a Kerrison rongeur or a curette, into the spinal canal without being aware of how narrow the canal is, or uneven decompression of the spinal cord during resection of the laminae can impinge on or distort the spinal cord and result in deterioration of neurological function. Several authors have pointed out that postoperative loss of neural function is a hazard of surgical intervention. Owing to the poor results of conventional laminectomy for cervical compression myelopathy related to the problems mentioned above, Kirita developed extensive simultaneous decompression laminectomy to avoid distortion of the spinal cord by the edges of the resected laminae. Hattori devised an expansive Z-shaped laminoplasty in which the posterior wall of the spinal canal was preserved by Z-plasty of the prepared laminae. This was an attempt to prevent the invasion of scar tissue, i. e., the so-called laminectomy membrane, which was believed to be a cause of late neurological regression. He also expected that the laminae reconstructed by Z-plasty would provide support for the spine. The introduction of high-speed air-drills allowed successful development of this procedure. In 1977, Hirabayashi introduced an epoch-making laminoplasty, the expansive open-door laminoplasty. He described the advantages of this procedure as: possibility of decompressing multiple levels of the spinal cord simultaneously, better postoperative support of the neck, allowing earlier mobilization of the patients, prevention of postoperative kyphotic deformity of the cervical spine, and reduced mobility of the cervical spine postoperatively, which helps to prevent late neurological deterioration and progression of OPLL. Subsequent to the Hirabayashi laminoplasty, various modifications and supplementary procedures have been devised for further improvement of the safety and efficacy of decompression, and for improved stability of the spine. Aims, advantages and disadvantages of laminoplasty: The aims of the laminoplasty are to expand the spinal canal, to secure spinal stability and to spare the protective function of the spine. Preservation of mobility of the spine is also a goal of this procedure for multiple level involvement. Decompression can be extended along the nerve root by facetectomy. Preservation of the posterior spinal structures permits reinsertion of the nuchal muscles and the spinal ligaments after they have been totally or partially detached. This prevents kyphosis or listhesis of the cervical spine, which often develops after laminectomy, particularly in subjects below 50 years of age. Reconstructive procedures for reattaching muscles and/or ligaments to the spinous processes are added to the laminoplasty, improving the dynamic or ligamentous stability of the spine.
    Notes: Zusammenfassung Trotz zunehmender Erfahrung mit der dorsalen Dekompression bei zervikaler spondylotischer Myelopathie (ZSM) kann eine erfolgreiche Laminektomie nur unter bestimmten Umständen garantiert werden [31, 32]. Weiterhin kann das Einführen chirurgischer Instrumente in einen engen Spinalkanal oder eine ungleichmäßige Dekompression des Rückenmarks zu neurologischen Funktionseinbußen führen [1, 4, 17]. Wegen dieser Probleme hat Kirita die Methode der extensiven simultanen dekomprimierenden Laminektomie entwickelt, um so eine Irritation des Myelons durch die scharfkantigen Ecken der resezierten Laminae zu vermeiden [14, 19]. Hattori entwickelte eine expansive z-förmige Laminoplastik, bei welcher die Hinterwand des Spinalkanals durch eine Z-Plastik der ausgedünnten Laminae erhalten wird [22]. Somit sollte ein Einsprossen von Narbengewebe verhindert und zusätzliche Stabilität für die Wirbelsäule gewährleistet werden. Hirabashi stellte 1977 ein epochemachendes Verfahren, die expansive „Open-door-Laminoplastik“, vor, welche durch später eingebrachte Modifikationen weitere Sicherheit und Effizienz gewonnen hat.
    Type of Medium: Electronic Resource
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