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  • 1985-1989  (12)
  • Polymer and Materials Science  (9)
  • serum polymorphism  (3)
  • 1
    Digitale Medien
    Digitale Medien
    Biosynthesis, processing, and extracellular release of α-l-fucosidase in lymphoid cell lines of different genetic origins (1988)
    Springer
    Biochemical genetics 26 (1988), S. 401-420 
    Details
    ISSN: 1573-4927
    Schlagwort(e): α-l-fucosidase ; lymphoid cells ; fucosidosis ; serum polymorphism
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract In humans, the quantity of α-l-fucosidase in serum is determined by heredity. The mechanism controlling levels of the enzyme in serum is unknown. Lymphoid cell lines derived from individuals with either low, intermediate, or high α-l-fucosidase in serum were established. Steady-state levels of intracellular and extracellular α-l-fucosidase as well as rates of synthesis and secretion of enzyme overlapped among the cell lines. Thus,vivo} serum phenotypes were not expressed in this system. No appreciable differences in the qualitative processing of newly made α-l-fucosidase were observed among these lymphoid cell lines. Cells pulse-labeled with35S-methionine from 0.25 to 2 hr had an intracellular form of enzyme with aM r=58,000. Cells pulsed for 1.5 hr and chased for 21 hr with unlabeled methionine had an intracellular form ofM r=60,000 and an extracellular form ofM r=62,000. All three enzyme forms were glycoproteins with a common polypeptide chain ofM r=52,000 but with different carbohydrate moieties. No evidence for a high molecular mass precursor form of α-l-fucosidase was found. Fucosidosis is a rare, inherited disease in which α-l-fucosidase activity in tissues and body fluids is low or absent. The mutations for fucosidosis and the serum polymorphism map separately. Lymphoid cells from two siblings with fucosidosis had 8-fold to 341-fold less intracellular α-l-fucosidase protein with 11-fold to 56-fold lower specific activities than control cells. Residual mutant enzyme was a glycoprotein with a polypeptide chain virtually the same size (M r=52,000) as control enzyme. However, residual mutant enzyme was hypoglycosylated and hypersecreted as compared to control enzyme.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02401794
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  • 2
    Digitale Medien
    Digitale Medien
    Specific activity of α-l-fucosidase in sera with phenotypes of either low, intermediate, or high total enzyme activity and in a fucosidosis serum (1986)
    Springer
    Biochemical genetics 24 (1986), S. 115-130 
    Details
    ISSN: 1573-4927
    Schlagwort(e): specific activity ; α-l-fucosidase ; serum polymorphism ; fucosidosis ; enzyme-linked immunoabsorbent assay (ELISA)
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract The quantity of α-l-fucosidase activity in human serum is determined by heredity. An individual may inherit either low, intermediate, or high serum enzyme activity. An enzyme-linked immunoabsorbent assay has been developed that can detect 0.3 ng of α-l-fucosidase protein. Enzyme protein in serum of 102 individuals ranged from 20 to 835 ng/ml. The group included individuals with low, intermediate, and high enzyme activity. The specific activity of α-l-fucosidase within this group was statistically the same (mean±SD=11,002±1051 U/mg). Thus, individuals with low and intermediate enzyme activity in serum had lower amounts of enzyme protein with the same specific activity as in individuals with high enzyme activity. Fucosidosis is a rare inherited disease in which α-l-fucosidase activity in tissues and body fluids is low or absent. The concentrations of enzyme protein in sera of a fucosidosis patient and parents were 76, 565, and 604 ng/ml, respectively, and the specific activities of enzyme were 1316, 8938, and 8858 U/mg, respectively. Thus, the fucosidosis serum probably contained a structurally altered enzyme with reduced catalytic activity. The somewhat low specific activities in the parents suggested that their sera contained both structurally altered and normal protein.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00502983
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  • 3
    Digitale Medien
    Digitale Medien
    Biosynthesis, processing, and extracellular release of α-l-fucosidase in lymphoid cell lines of different genetic origins (1988)
    Springer
    Biochemical genetics 26 (1988), S. 401-420 
    Details
    ISSN: 1573-4927
    Schlagwort(e): α-l-fucosidase ; lymphoid cells ; fucosidosis ; serum polymorphism
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract In humans, the quantity of α-l-fucosidase in serum is determined by heredity. The mechanism controlling levels of the enzyme in serum is unknown. Lymphoid cell lines derived from individuals with either low, intermediate, or high α-l-fucosidase in serum were established. Steady-state levels of intracellular and extracellular α-l-fucosidase as well as rates of synthesis and secretion of enzyme overlapped among the cell lines. Thus,vivo} serum phenotypes were not expressed in this system. No appreciable differences in the qualitative processing of newly made α-l-fucosidase were observed among these lymphoid cell lines. Cells pulse-labeled with35S-methionine from 0.25 to 2 hr had an intracellular form of enzyme with aM r=58,000. Cells pulsed for 1.5 hr and chased for 21 hr with unlabeled methionine had an intracellular form ofM r=60,000 and an extracellular form ofM r=62,000. All three enzyme forms were glycoproteins with a common polypeptide chain ofM r=52,000 but with different carbohydrate moieties. No evidence for a high molecular mass precursor form of α-l-fucosidase was found. Fucosidosis is a rare, inherited disease in which α-l-fucosidase activity in tissues and body fluids is low or absent. The mutations for fucosidosis and the serum polymorphism map separately. Lymphoid cells from two siblings with fucosidosis had 8-fold to 341-fold less intracellular α-l-fucosidase protein with 11-fold to 56-fold lower specific activities than control cells. Residual mutant enzyme was a glycoprotein with a polypeptide chain virtually the same size (M r=52,000) as control enzyme. However, residual mutant enzyme was hypoglycosylated and hypersecreted as compared to control enzyme.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00554076
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  • 4
    Digitale Medien
    Digitale Medien
    13C-NMR spectra of cis-polymyrcene and cis-polyfarnesene (1988)
    Bognor Regis [u.a.] : Wiley-Blackwell
    Journal of Polymer Science Part A: Polymer Chemistry 26 (1988), S. 71-77 
    Details
    ISSN: 0887-624X
    Schlagwort(e): Chemistry ; Polymer and Materials Science
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie
    Notizen: Homopolymers of myrcene and farnesene were prepared anionically in pure cyclohexane. The microstructure, determined from an analysis of the 13C-NMR spectrum and spin-lattice relaxation times, indicates the polymers are at least 85% cis-1,4, 10% cis-3,4, and under 3% trans-1,4.
    Zusätzliches Material: 1 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/pola.1988.080260107
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  • 5
    Digitale Medien
    Digitale Medien
    Structure of fibrous cellulose acetate: X-ray diffraction, positron annihilation and electron microscopy investigations (1987)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 33 (1987), S. 95-106 
    Details
    ISSN: 0021-8995
    Schlagwort(e): Chemistry ; Polymer and Materials Science
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie , Maschinenbau , Physik
    Notizen: X-ray diffraction, positron annihilation, electron microscopy and bulk density measurements are reported as a function of the degree of acetylation for a linters cotton. Heterogeneous reaction proceeds with a retention of ordered structure in the fibers. Substitution does increase the extent of disorder but is not sufficiently extensive to disrupt completely order at the fiber, fibril, micro fibril, and crystallite levels. The generation of disorder is associated with an increase in the interfibrillar distance, a breakup of the microfibrillar structure without a total disruption of the microcrystalline structure.
    Zusätzliches Material: 9 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/app.1987.070330109
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  • 6
    Digitale Medien
    Digitale Medien
    A comparative study of the biologic and immunologic response to medical devices derived from dermal collagen (1986)
    Hoboken, NJ : Wiley-Blackwell
    Journal of Biomedical Materials Research 20 (1986), S. 109-120 
    Details
    ISSN: 0021-9304
    Schlagwort(e): Chemistry ; Polymer and Materials Science
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Medizin , Technik allgemein
    Notizen: We examined collagen materials for soft tissue augmentation [Zyderm® Collagen Implant (ZCI), glutaraldehyde cross-linked (GAX) collagen, and Koken Atelocollagen (Atelocollagen)]; hemostatic collagens [Gelfoam® Gelatin Powder (Gelfoam), Avitene® Microfibrillar Collagen Hemostat (Avitene), and Collastat® Collagen Hemostat (Collastat)]; and reconstituted, intact fibrillar collagen from bovine skin in a subcutaneous guinea pig model. After 11, 25, and 39 days in situ, explants from animals injected with GAX collagen demonstrated greater wet-weight persistence than all other materials. Conversely, at all time points, the explants of Atelocollagen were the least persistent. Following 25 days in vivo, explants were examined using differential scanning calorimetry; ZCI and Atelocollagen displayed thermal transition temperatures of 58°C. Avitene and Gelfoam explants displayed transition points of 30°C and 32°C, indicating denatured or cleaved collagen. By contrast, GAX collagen explants had a high (68°C) transition temperature, reflecting its cross-linking. With respect to immunogenicity, day 39 sera from ZCI treated animals showed significantly lower titers in the ELISA to their respective implant collagen than all other groups examined, while antibody activity in the GAX collagen, Gelfoam, Atelocollagen, and intact collagen groups were not significantly different. Collastat elicited antibodies with a greater affinity than observed in these previous groups. Sera from Avitene treated animals demonstrated the highest antibody levels and were the only sera which reacted with bovine serum albumin. Thus, Avitene was the most immunogenic of the collagen materials examined, while GAX collagen demonstrated the greatest persistence and minimal immunogenicity, and ZCI was the least immunogenic.
    Zusätzliches Material: 3 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/jbm.820200110
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  • 7
    Digitale Medien
    Digitale Medien
    Wound healing using a collagen matrix: Effect of DC electrical stimulation (1988)
    Hoboken, NJ : Wiley-Blackwell
    Journal of Biomedical Materials Research 22 (1988), S. 191-206 
    Details
    ISSN: 0021-9304
    Schlagwort(e): Chemistry ; Polymer and Materials Science
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Medizin , Technik allgemein
    Notizen: Rapid fibroblast ingrowth and collagen deposition occurs in a reconstituted type I collagen matrix that is implanted on fullthickness excised animal dermal wounds. The purpose of this study is to evaluate the effects of direct current stimulation on dermal fibroblast ingrowth using carbon fiber electrodes incorporated into a collagen sponge matrix. Preliminary results suggest that fibroblast ingrowth and collagen fiber alignment are increased in collagen sponges stimulated with direct currents between 20 and 100 μA. Maximum fibroblast ingrowth into the collagen sponge is observed near the cathode at a current of 100 μA. These results suggest that electrical stimulation combined with a collagen matrix may be a method to enhance the healing of chronic dermal wounds.
    Zusätzliches Material: 6 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/jbm.820221310
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  • 8
    Digitale Medien
    Digitale Medien
    Material properties of living soft tissue composites (1988)
    Hoboken, NJ : Wiley-Blackwell
    Journal of Biomedical Materials Research 22 (1988), S. 311-326 
    Details
    ISSN: 0021-9304
    Schlagwort(e): Chemistry ; Polymer and Materials Science
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Medizin , Technik allgemein
    Notizen: Collagen sponges seeded with fibroblasts have been used as a soft tissue substitute in wound healing applications. This biomaterial is a good in vitro analog of a connective tissue. Therefore, analysis of the properties of this material may be useful for theoretically modeling soft tissues. Stress-strain curves for such cell-seeded collagen sponges were measured to determine composite stiffness and ultimate tensile strength. Theoretical modeling was done by defining a particle-reinforced matrix using the composite sphere model. A system of uniaxially oriented fibers was then introduced to this equivalent homogeneous media and material properties were determined using the composite cylinder model. Geometric averaging was performed to yield the stiffness and Poissons' ratio for a composite with randomly oriented fibers. Inputs to the model were constituent material properties, cell volume fraction, and fiber volume fraction. From theoretical results, material properties of soft tissues and their substitutes depend on fiber mechanical properties and volume fraction and not cellular mechanical properties and volume fraction. Therefore, the increase in experimentally observed composite stiffness with increased cell number was due to deposition of newly synthesized stiffer collagen fibers, and not due to the physical presence of cells themselves.
    Zusätzliches Material: 5 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/jbm.820221409
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  • 9
    Digitale Medien
    Digitale Medien
    Morphology and cation local structure in a blend of copper-neutralized carboxy-terminated polybutadiene and poly(styrene-co-4-vinylpyridine) (1989)
    Bognor Regis [u.a.] : Wiley-Blackwell
    Journal of Polymer Science Part B: Polymer Physics 27 (1989), S. 1911-1925 
    Details
    ISSN: 0887-6266
    Schlagwort(e): Chemistry ; Polymer and Materials Science
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie , Physik
    Notizen: Carboxy-terminated polybutadiene neutralized with Cu2+ (CuPBD) and its blend with poly(styrene-co-4-vinylpyridine) (SVP) were examined by differential scanning calorimetry (DSC), transmission electron microscopy (TEM), small-angle x-ray scattering (SAXS), and extended x-ray absorption fine structure (EXAFS) spectroscopy. The DSC results indicate that in the blend substantial mixing occurs in the CuPBD-rich phase, although complete miscibility is not achieved, and the SVP-rich phase remains relatively pure. The TEM micrographs indicate that the morphology, while irregular, is reasonably described as bicontinuous, with a domain size of order 100 nm. The SAXS patterns show that the ionic aggregates present in CuPBD are destroyed upon blending, which is interpreted as being due to steric hindrances between ionic groups coordinated to vinylpyridine nitrogens. The EXAFS radial structure function of the blend exhibits a marked decrease in the Cu-Cu peak in comparison with CuPBD, indicating a change in local structure upon blending. The results indicate that some of the SVP is miscible with CuPBD owing to complexation between the pendant pyridine groups and the Cu2+ ions, which disrupts the ionic aggregates. However, the two materials are not fully miscible, leading to a rather coarse two-phase morphology.
    Zusätzliches Material: 8 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/polb.1989.090270909
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  • 10
    Digitale Medien
    Digitale Medien
    Multiple denaturational transitions in fibrillar collagen (1986)
    New York : Wiley-Blackwell
    Biopolymers 25 (1986), S. 1875-1893 
    Details
    ISSN: 0006-3525
    Schlagwort(e): Chemistry ; Polymer and Materials Science
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie
    Notizen: The heat denaturation of pepsinized bovine nonfibrillar and fibrillar collagen was studied by differential scanning calorimetry. For fibrillar preparations that had been rapidly precipitated with stirring at low ionic strength, then resuspended at physiological ionic strength, multiple denaturational transitions were observed. At heating rates of 10°C/min, melting endotherms occurred at about 44, 50, 53, and 57°C. Fibrillar collagen that was slowly gelled without stirring at physiological ionic strength exhibited a similar series of endotherms, but the lower melting transitions were less conspicuous. In contrast, nonfibrillar bovine collagen in acidic solution showed only a single denaturational transition at 40°C. Nonfibrillar solutions at pH 7, to which inhibitors of fibrillogenesis were added, showed a major endotherm as high as 46°C. These results suggest that reconstituted fibrillar collagen contains a heterogeneous fibril population, possibly including molecules in a nonfibrillar state. It was proposed that the multiple melting endotherms of such preparations were due to sequential melting of molecular and fibril classes, each with a distinct melting temperature. The fibrillar classes may represent three or more types of banded and nonbanded species that differ from each other in packing order, collagen concentration, and possibly also in fibril width and level of cross-linking.
    Zusätzliches Material: 8 Ill.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/bip.360251006
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