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  • Polymer and Materials Science  (17)
  • Phaseolus vulgaris  (6)
  • Cell & Developmental Biology  (5)
  • 1
    Electronic Resource
    Electronic Resource
    Bean lectins IV: genetic variation in the non-denatured structure of lectins from different Phaseolus vulgaris L. cultivars (1984)
    Springer
    Theoretical and applied genetics 67 (1984), S. 547-552 
    Details
    ISSN: 1432-2242
    Keywords: Phaseolus vulgaris ; Lectins ; Albumin ; Globulin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Variation in the native conformation of bean lectins was examined using electrophoresis of non-denatured total protein extracts and purified albumin and globulin lectin. The observed variation was related to the genetic variation reported previously for lectin polypeptide composition as revealed by two-dimensional isoelectricfocusing-sodium dodecyl sulfate polyacrylamide gel electrophoresis (IEF-SDS/PAGE). When eleven cultivars with different IEF-SDS/PAGE lectin polypeptide compositions were compared, eight had unique non-denatured lectin patterns and three had identical patterns. For some cultivars differences in non-denatured lectin patterns were observed between the purified albumin and globulin lectin preparations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00264902
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  • 2
    Electronic Resource
    Electronic Resource
    Bean lectins (1985)
    Springer
    Theoretical and applied genetics 70 (1985), S. 22-31 
    Details
    ISSN: 1432-2242
    Keywords: Phaseolus vulgaris ; Lectin ; Phaseolin ; Quantitative variation ; Immunoelectrophoresis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Seeds of forty bean cultivars having different lectin types based on two-dimensional isoelectric focusing-sodium dodecyl sulfate polyacrylamide gel electrophoresis (IEF-SDS/PAGE) were analyzed for quantities of lectin, phaseolin and total protein. Significant differences were found among groups of cultivars with different lectin types for the quantity of lectin and phaseolin. Cultivars with more complex lectin types based on IEF-SDS/PAGE tended to have higher quantities of lectin and lower quantities of phaseolin than cultivars with simple lectin types. An association between lectin type and the quantity of lectin and phaseolin was found also in the seeds of F2 plants that segregated in a Mendelian fashion for two lectin types. Seeds from plants with the complex lectin type had more lectin and less phaseolin than seeds from plants with the simple lectin type. Therefore, the genes controlling qualitative lectin variation also may influence the quantitative variation of lectin and phaseolin. The results of this study are related to other studies on the quantitative variation for seed proteins and to the possible molecular basis for variation in the quantity of lectins in beans.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00264478
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  • 3
    Electronic Resource
    Electronic Resource
    Bean lectins (1982)
    Springer
    Theoretical and applied genetics 62 (1982), S. 361-367 
    Details
    ISSN: 1432-2242
    Keywords: Phaseolus vulgaris ; Lectins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The relationship between the polypeptide composition and the agglutination behaviour of the lectin-containing G2/albumin protein groups has allowed the identification of the active lectin polypeptides in different cultivars of Phaseolus vulgaris (Brown et al. accompanying paper). These results were used to ascertain the particular G2/albumin group contained in the various lectin sources used previously for the purification of lectin proteins. Many studies were found to have included lectin sources which contained the same G2/albumin pattern (TG2) and this common denominator has permitted the direct comparison of the properties reported for these purified lectins. Thus, much of the extensive literature on bean lectins is concurred.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00275105
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  • 4
    Electronic Resource
    Electronic Resource
    Genetic variation in the subunits of globulin-1 storage protein of French bean (1981)
    Springer
    Theoretical and applied genetics 59 (1981), S. 83-88 
    Details
    ISSN: 1432-2242
    Keywords: Phaseolus vulgaris ; Storage proteins ; Electrophoresis ; Genetic variation ; Banding types
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Charge and molecular weight heterogeneity of globulin-1 (G1) polypeptides of the bean, Phaseolus vulgaris L., were revealed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). Different bean cultivars were classified into three groups: ‘Tendergreen’, ‘Sanilac’, and ‘Contender’ on the basis of their protein subunit composition. Nine distinct major bands: α51,α49, α48.5,β48T, β48S, β47, γ45.5, γ45S, and γ45C, and two minor bands: γ46T and γ46S were found to account for the three profiles seen on one-dimensional SDS-PAGE. Two-dimensional analysis revealed these eleven protein bands to be composed of a minimum of fourteen distinct protein subunits. The ‘Tendergreen’ and ‘Sanilac’ types differ in their G1 polypeptide composition. The protein patterns of the ‘Contender’ types are intermediate, containing many protein subunits found in the patterns of the ‘Tendergreen’ and ‘Sanilac’ types suggesting a genetic and evolutionary relationship.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00285895
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  • 5
    Electronic Resource
    Electronic Resource
    Linkage relationships between genes controlling seed proteins in French bean (1981)
    Springer
    Theoretical and applied genetics 60 (1981), S. 251-259 
    Details
    ISSN: 1432-2242
    Keywords: Phaseolus vulgaris ; Phaseolin ; Seed proteins ; Electrophoresis ; Linkage
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The inheritance of phaseolin and globulin-2 (G2)/albumin polypeptides was investigated in crosses involving varieties which exhibited the three electrophoretic banding patterns of phaseolin found in French bean. ‘Total’ seed protein extracts of single seeds of the F1 and F2 generations from the crosses: ‘Sanilac’ × ‘Contender’, ‘BBL 240’ × ‘Contender’, and ‘Sanilac’ × ‘BBL 240’ were analyzed by two-dimensional electrophoresis. Segregation of the genes controlling phaseolin and G2/albumin polypeptides, and those controlling a further five groups of seed proteins (A, B, D, E, and F) were observed. No recombinant electrophoretic phenotypes were seen for phaseolin or G2/albumin polypeptides suggesting that the genes controlling each of these groups of polypeptides are closely linked and segregate like single Mendelian genes. The phaseolin genes and G2/albumin genes were not linked to each other. The group of genes controlling phaseolin polypeptides were linked to those controlling group B proteins, and those controlling G2/albumin polypeptides were linked to those controlling group F proteins.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02342545
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  • 6
    Electronic Resource
    Electronic Resource
    Bean lectins (1982)
    Springer
    Theoretical and applied genetics 62 (1982), S. 263-271 
    Details
    ISSN: 1432-2242
    Keywords: Phaseolus vulgaris ; Seed protein ; Lectins ; Electrophoresis ; Agglutination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Single seeds of over 100 bean cultivars were analyzed by two-dimensional electrophoresis. The cultivars could be classified into eight groups by virtue of their G2/albumin electrophoretic patterns: TG2, SG2, VG2, PrG2, BG2, MG2, PG2, and PiG2, The polypeptide compositions of these types were largely inter-related having particular polypeptides in common. It was possible to correlate the G2/albumin patterns with agglutinating activity of cow and rabbit blood cells as measured by the agglutination ratio (minimum concentration of extract required to agglutinate cow blood cells: minimum concentration of extract required to agglutinate rabbit blood cells). The active lectin polypeptides were identified by extracting lectins from agglutinated erythrocytes and by comparing the qualitative similarities and differences of the G2/albumin patterns and their agglutination activities. A reference catalogue of over 100 bean cultivars giving their phaseolin and G2/albumin electrophoretic patterns, and agglutination ratios is presented.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00276249
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  • 7
    Electronic Resource
    Electronic Resource
    Observations on preadipocytes and their distribution patterns in rat adipose tissue (1981)
    New York, NY : Wiley-Blackwell
    Journal of Morphology 168 (1981), S. 281-288 
    Details
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Microscopic examination of adipocytes isolated from adult rat epididymal adipose tissue revealed numerous small cells (〈 10 μm) morphologically similar to larger adipocytes. These small adipocytes appear identical to a new classification of adipose cells termed preadipocytes. Electron micrographs of these preadipocytes revealed examples of cells 〈 10 μm in diameter in various stages of maturation and lipid accumulation. The percent distribution pattern of these small adipocytes was not significantly altered by exercise although exercise shifted the distribution patterns of the larger cells (〉 30 μm) toward a smaller mean cell size. The quantitative significance of preadipocytes is not established but these preliminary observations indicate that adipocytes 〈 10 μm in diameter may account for a numerically greater proportion of the total adipocytes observed in collagenase isolated preparations than heretofore recognized, although their contribution to total adipose mass is probably negligible.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jmor.1051680304
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  • 8
    Electronic Resource
    Electronic Resource
    Effects of gamma radiation on two aromatic polysulfones (1975)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Polymer Science 19 (1975), S. 405-417 
    Details
    ISSN: 0021-8995
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Two aromatic polysulfones, poly(oxy-1,4-phenylenesulfonyl-1,4-phenyleneoxy-1,4-phenyleneisopropylidene-1,4-phenylene) (I) and poly (oxy-1,4-phenylenesulfonyl-1,4-phenylene) (II), undergo crosslinking and chain scission at 30°C during γ-irradiation, the former being predominant in vacuum and the latter in air. Both processes occurred more readily in I, which contains isopropylidene linkages. Gel measurements gave G(crosslink) = 0.051, G(scission) = 0.012 for this polymer at 30°C in vacuum. Increased irradiation temperatures resulted in higher crosslinking and gas yields, especially above the glass transition temperature. The tensile strength, flexural strength, and modulus of I were unaffected by γ-irradiation up to about 50 Mrad in air, but the strength decreased markedly at higher doses. The elongation at break decreased progressively with dose. For both polymers, G(gas) = 0.04 at 30°C with the main products being SO2, H2, CO2, CH4, and H2O.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/app.1975.070190208
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  • 9
    Electronic Resource
    Electronic Resource
    The effect of hydrogen chloride on the corrosion of an FeCrAlY alloy in simulated coal gasifier atmospheres (1989)
    Weinheim [u.a.] : Wiley-Blackwell
    Materials and Corrosion/Werkstoffe und Korrosion 40 (1989), S. 175-181 
    Details
    ISSN: 0947-5117
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Description / Table of Contents: Einfluß von Chlorwasserstoff auf die Korrosion einer FeCrAlY-Legierung in einer simulierten KohlevergasungsatmosphäreEie Chrom-Aluminium-Yttrium-Stahl wurde einer simulierten Kohlevergasungsatmosphäre ausgesetzt die 1000 ppm bzw. 2200 ppm Chlorwasserstoff enthielt und deren Temperatur 450 °C betrug. Durch Erhöhen des Chlorwasserstoffgehalts werden die Reaktionsgeschwindigkeit erhöht und die Mikrostruktur sowie die Zusammensetzung des Korrosionsprodukts beträchtlich verändert. Für diese Ergebnisse werden versuchsweise Mechanismen postuliert, nach denen Metallchloride über die Gasphase transportiert werden.
    Notes: An iron chromium aluminium yttrium steel was exposed to a simulated coal gasifier atmosphere containing 1000 ppm and 2200 ppm hydrogen chloride at 450 °C. Increasing hydrogen chloride content was found to accelerate reaction rates, and significantly alter the microstructure and composition of the corrosion product. Tentative explanations for these results, involving vapour phase transport of metal chlorides are proposed.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/maco.19890400312
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  • 10
    Electronic Resource
    Electronic Resource
    Renal microvasculature of the rainbow trout, Salmo gairdneri: Scanning electron microscopy of corrosion casts of glomeruli (1985)
    New York, NY [u.a.] : Wiley-Blackwell
    The @Anatomical Record 213 (1985), S. 505-513 
    Details
    ISSN: 0003-276X
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: Scanning electron microscopy of corrosion casts of blood vessels permits detailed and accurate study of the microcirculation. The present study examined the renal microvasculature of the rainbow trout, Salmo gairdneri. The conventional picture of a glomerulus with one afferent arteriole was common, but glomeruli were often supplied by two afferent arterioles. In the majority of these, the intrarenal artery gave rise to a single afferent arteriole that branched to form two smaller vessels before reaching the glomerulus. Glomeruli with two afferent arterioles that arose independently from the intrarenal artery also occurred. The majority of glomeruli had a single efferent arteriole, but a proportion of glomeruli had two efferent arterioles. Efferent arterioles were smaller in diameter than the afferent arterioles. The glomerular capillaries were arranged in lobules, with few anastomoses between lobules, so that, for glomeruli with two afferent or two efferent arterioles, vascular perfusion and thus filtration within discrete lobules is probable.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/ar.1092130405
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