ISSN:
1432-1912
Schlagwort(e):
Cardiac Glycosides
;
Proscillaridin
;
Antibodies
;
Radioimmunoassay
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Medizin
Notizen:
Summary Rabbits were immunized for 10 weeks with a complex between the bufadienolide glycoside proscillaridin (p.) and human serum albumin. Antibodies were obtained with a binding capacity of 47 ng of p. per μl of undiluted serum. The antibodies migrated with the Ig A immunoglobulin fraction. Precipitation of the antigen-antibody complex was demonstrated by agargel electrophoresis, immunoelectrophoresis and immunodiffusion. When added to a 86Rb-erythrocyte system the antiserum prevented the inhibition of Rb-uptake due to p. with a linear doseresponse relationship. Displacement studies with different chemically related steroids were performed using a 3H-p.-antibody system in order to test the specificity of the antibody. Changes in the conformation and the substituents in position 3 and 4 did not influence antibody binding to a major degree. Quabain, digoxin and digitoxin, however, had a much lower affinity to the antibody. The radioimmunological system described is able to detect amounts of p. above 15 ng.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF00503976
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