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  • 1
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/General Subjects 924 (1987), S. 312-318 
    ISSN: 0304-4165
    Keywords: (Human articular cartilage) ; Cathepsin G ; Elastase ; Proteinase inhibitor ; Rheumatoid arthritis
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine , Physics
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1437-160X
    Keywords: Alpha 1-proteinase inhibitor ; Leucocyte elastase ; Cartilage ; Rheumatoid arthritis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Synovial fluids of patients suffering from rheumatoid arthritis contain elevated levels of granulocyte (PMN) elastase in complex with alpha 1-proteinase inhibitor (alpha 1-PI), whereas free-elastase activity is usually not detectable. This absence of free enzymatic activity in joint effusions has cast some doubt on the pathophysiological relevance of PMN elastase in inflammatory joint destruction. Our in vitro experiments using bovine nasal cartilage demonstrate that incubation with elastase and alpha 1-PI in equimolar concentrations to or even in excess of the serum proteinase inhibitor resulted in significant tissue destruction as assessed by histological staining for proteoglycans, release of uronic acid from the matrix and loss of mechanical stability. Though in the supernatants containing alpha 1-PI, free-elastase activity was not detectable, immunofluorescent staining for elastase evidenced penetration of the enzyme into the matrix. Simultaneous measurements of the incubation media employing a sandwich enzyme-linked immunoadsorption assay (ELISA) revealed PMN elastase in complex with alpha 1-PI but without correlation to the parameters of tissue degradation. In comparison with the results obtained using the chromogenic substrate Suc-Ala-Ala-Ala-pNA (SAPA) for titration of alpha 1-PI against elastase, the employment of cartilage matrix showed that a fourfold increase in inhibitor concentration was necessary to achieve 100% enzyme inhibition. Hence, cartilage surface obviously interferes with the interaction between alpha 1-PI and elastase. Measurements of elastase-inhibitor concentrations or free enzymatic activity in synovial fluid seem to have limited value in predicting cartilage destruction.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Surveys in geophysics 17 (1996), S. 347-360 
    ISSN: 1573-0956
    Keywords: Ultrasonic waves ; Attenuation ; Velocity ; Microstructure ; Thermal cracking ; Local fluid flow
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract The frequency dependent mechanism of local fluid flow was found to be the decisive absorption and dispersion mechanism in fluid containing sandstones. In the ultrasonic frequency range local fluid flow and grain surface effects control the behaviour of highly porous and highly permeable rock if a pore fluid is present. Both mechanisms depend less on macroscopic rock parameters like porosity and permeability than essentially on microscopic parameters like crack size, crack density and grain contact properties. To demonstrate directly the important influence of the microstructure on the rock elastic and anelastic properties the microstructure of a sandstone was artificially changed by thermal cracking. The cracked rock exhibits a clearly changed behaviour at low uniaxial as well as at high hydrostatic pressure despite small changes of porosity and permeability. Fluid effects increase due to cracking. The experimental results are explained by means of a rock, model and local fluid flow. These results emphasize that it is the microstructure which controls the elastic and anelastic rock behaviour, even at high hydrostatic pressure.
    Type of Medium: Electronic Resource
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