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  • Digitale Medien  (12)
  • Phaseolus vulgaris  (6)
  • Solanum tuberosum  (6)
  • 1
    Digitale Medien
    Digitale Medien
    Bean lectins IV: genetic variation in the non-denatured structure of lectins from different Phaseolus vulgaris L. cultivars (1984)
    Springer
    Theoretical and applied genetics 67 (1984), S. 547-552 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Phaseolus vulgaris ; Lectins ; Albumin ; Globulin
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Variation in the native conformation of bean lectins was examined using electrophoresis of non-denatured total protein extracts and purified albumin and globulin lectin. The observed variation was related to the genetic variation reported previously for lectin polypeptide composition as revealed by two-dimensional isoelectricfocusing-sodium dodecyl sulfate polyacrylamide gel electrophoresis (IEF-SDS/PAGE). When eleven cultivars with different IEF-SDS/PAGE lectin polypeptide compositions were compared, eight had unique non-denatured lectin patterns and three had identical patterns. For some cultivars differences in non-denatured lectin patterns were observed between the purified albumin and globulin lectin preparations.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00264902
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  • 2
    Digitale Medien
    Digitale Medien
    Selection for yield and yield components in the early generations of a potato breeding programme (1986)
    Springer
    Theoretical and applied genetics 73 (1986), S. 218-222 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Solanum tuberosum ; Selection efficiency ; Potato breeding ; Yield ; Yield components
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary A number of unselected potato (Solanum tuberosum L.) clones were grown at two locations (a seed site and a ware site) in three consecutive years. The repeatability of total yield and yield components in the first two clonal years was compared with the same characters recorded in the third clonal year. Selection for yield in the first clonal year was only marginally more effective than a random reduction in number of genotypes, while selection in the second clonal year appeared to be somewhat more effective as judged by performance in the third clonal year. The inefficiency of selection in the first clonal year was ascribed, at least in part, to the inaccuracy of yield assessment as well as the “carry-over” effect of the mother tubers. Correlations of total yield were higher between different years in the same location than between different locations. Selection under growing conditions suitable for production of seed tubers tended to result in selection of early maturing clones which would not necessarily be optimal for ware growing conditions.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00289277
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  • 3
    Digitale Medien
    Digitale Medien
    Cross prediction in a potato breeding programme by evaluation of parental material (1989)
    Springer
    Theoretical and applied genetics 77 (1989), S. 246-252 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Solanum tuberosum ; Parental value ; Univariate cross prediction ; Mid-parent ; Mid-self value
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary It is shown that it is possible to obtain an indication of the parental value of heterozygous cultivars of potatoes for the characters breeders' preference, total tuber weight, mean tuber weight and number of tubers. Three methods of predicting the characteristics of progeny produced from particular parental cultivars were examined, namely: univariate cross prediction (based on mean and variance), mid-parent values and mid-self values. All provided some indication of which crosses would give superior progeny, but univariate cross prediction gave rankings which correlated most highly with observed performance in the second clonal year for the characters breeders' preference and total tuber weight. Interestingly, mid-self values gave the best predictions in the case of mean tuber weight and number of tubers. It is suggested that such predictions of ‘parental value’, especially when based on univariate cross prediction at the seedling stage, are worth carrying out in practical breeding programmes.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00266194
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  • 4
    Digitale Medien
    Digitale Medien
    Bean lectins (1985)
    Springer
    Theoretical and applied genetics 70 (1985), S. 22-31 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Phaseolus vulgaris ; Lectin ; Phaseolin ; Quantitative variation ; Immunoelectrophoresis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Seeds of forty bean cultivars having different lectin types based on two-dimensional isoelectric focusing-sodium dodecyl sulfate polyacrylamide gel electrophoresis (IEF-SDS/PAGE) were analyzed for quantities of lectin, phaseolin and total protein. Significant differences were found among groups of cultivars with different lectin types for the quantity of lectin and phaseolin. Cultivars with more complex lectin types based on IEF-SDS/PAGE tended to have higher quantities of lectin and lower quantities of phaseolin than cultivars with simple lectin types. An association between lectin type and the quantity of lectin and phaseolin was found also in the seeds of F2 plants that segregated in a Mendelian fashion for two lectin types. Seeds from plants with the complex lectin type had more lectin and less phaseolin than seeds from plants with the simple lectin type. Therefore, the genes controlling qualitative lectin variation also may influence the quantitative variation of lectin and phaseolin. The results of this study are related to other studies on the quantitative variation for seed proteins and to the possible molecular basis for variation in the quantity of lectins in beans.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00264478
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  • 5
    Digitale Medien
    Digitale Medien
    The use of cross prediction methods in a practical potato breeding programme (1988)
    Springer
    Theoretical and applied genetics 76 (1988), S. 33-38 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Potatoes ; Solanum tuberosum ; Cross prediction ; Breeding
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Most previous studies on cross prediction methods have examined relatively few crosses, particularly in relation to the numbers involved in most breeding programmes. In this paper the feasibility of using cross prediction methods was examined in a practical potato (Solanum tuberosum) breeding scheme by the analyses of progeny from 52 crosses. The variate considered was breeder's preference, a visual assessment made of the harvested tubers to estimate their commercial potential. The results showed that it was possible to identify the superior crosses. Cross prediction based simply on the mean preference scores, averaged over scorers and clones within progenies, estimated on seedlings or first clonal year plants, provided the best estimate of a progeny's performance in the third clonal generation. Predictions based on the expected proportion of clones that would transgress a given target value also provided a good indication of a progeny's potential. The poorest prediction was obtained by using the observed frequency of desirable clones in a progeny sample. The implications for potato breeding are discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00288828
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  • 6
    Digitale Medien
    Digitale Medien
    Bean lectins (1982)
    Springer
    Theoretical and applied genetics 62 (1982), S. 361-367 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Phaseolus vulgaris ; Lectins
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The relationship between the polypeptide composition and the agglutination behaviour of the lectin-containing G2/albumin protein groups has allowed the identification of the active lectin polypeptides in different cultivars of Phaseolus vulgaris (Brown et al. accompanying paper). These results were used to ascertain the particular G2/albumin group contained in the various lectin sources used previously for the purification of lectin proteins. Many studies were found to have included lectin sources which contained the same G2/albumin pattern (TG2) and this common denominator has permitted the direct comparison of the properties reported for these purified lectins. Thus, much of the extensive literature on bean lectins is concurred.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00275105
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  • 7
    Digitale Medien
    Digitale Medien
    Genetic variation in the subunits of globulin-1 storage protein of French bean (1981)
    Springer
    Theoretical and applied genetics 59 (1981), S. 83-88 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Phaseolus vulgaris ; Storage proteins ; Electrophoresis ; Genetic variation ; Banding types
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Charge and molecular weight heterogeneity of globulin-1 (G1) polypeptides of the bean, Phaseolus vulgaris L., were revealed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE). Different bean cultivars were classified into three groups: ‘Tendergreen’, ‘Sanilac’, and ‘Contender’ on the basis of their protein subunit composition. Nine distinct major bands: α51,α49, α48.5,β48T, β48S, β47, γ45.5, γ45S, and γ45C, and two minor bands: γ46T and γ46S were found to account for the three profiles seen on one-dimensional SDS-PAGE. Two-dimensional analysis revealed these eleven protein bands to be composed of a minimum of fourteen distinct protein subunits. The ‘Tendergreen’ and ‘Sanilac’ types differ in their G1 polypeptide composition. The protein patterns of the ‘Contender’ types are intermediate, containing many protein subunits found in the patterns of the ‘Tendergreen’ and ‘Sanilac’ types suggesting a genetic and evolutionary relationship.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00285895
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  • 8
    Digitale Medien
    Digitale Medien
    Linkage relationships between genes controlling seed proteins in French bean (1981)
    Springer
    Theoretical and applied genetics 60 (1981), S. 251-259 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Phaseolus vulgaris ; Phaseolin ; Seed proteins ; Electrophoresis ; Linkage
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The inheritance of phaseolin and globulin-2 (G2)/albumin polypeptides was investigated in crosses involving varieties which exhibited the three electrophoretic banding patterns of phaseolin found in French bean. ‘Total’ seed protein extracts of single seeds of the F1 and F2 generations from the crosses: ‘Sanilac’ × ‘Contender’, ‘BBL 240’ × ‘Contender’, and ‘Sanilac’ × ‘BBL 240’ were analyzed by two-dimensional electrophoresis. Segregation of the genes controlling phaseolin and G2/albumin polypeptides, and those controlling a further five groups of seed proteins (A, B, D, E, and F) were observed. No recombinant electrophoretic phenotypes were seen for phaseolin or G2/albumin polypeptides suggesting that the genes controlling each of these groups of polypeptides are closely linked and segregate like single Mendelian genes. The phaseolin genes and G2/albumin genes were not linked to each other. The group of genes controlling phaseolin polypeptides were linked to those controlling group B proteins, and those controlling G2/albumin polypeptides were linked to those controlling group F proteins.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF02342545
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  • 9
    Digitale Medien
    Digitale Medien
    Bean lectins (1982)
    Springer
    Theoretical and applied genetics 62 (1982), S. 263-271 
    Details
    ISSN: 1432-2242
    Schlagwort(e): Phaseolus vulgaris ; Seed protein ; Lectins ; Electrophoresis ; Agglutination
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Single seeds of over 100 bean cultivars were analyzed by two-dimensional electrophoresis. The cultivars could be classified into eight groups by virtue of their G2/albumin electrophoretic patterns: TG2, SG2, VG2, PrG2, BG2, MG2, PG2, and PiG2, The polypeptide compositions of these types were largely inter-related having particular polypeptides in common. It was possible to correlate the G2/albumin patterns with agglutinating activity of cow and rabbit blood cells as measured by the agglutination ratio (minimum concentration of extract required to agglutinate cow blood cells: minimum concentration of extract required to agglutinate rabbit blood cells). The active lectin polypeptides were identified by extracting lectins from agglutinated erythrocytes and by comparing the qualitative similarities and differences of the G2/albumin patterns and their agglutination activities. A reference catalogue of over 100 bean cultivars giving their phaseolin and G2/albumin electrophoretic patterns, and agglutination ratios is presented.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00276249
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  • 10
    Digitale Medien
    Digitale Medien
    Identifying superior parents in a potato breeding program using cross prediction techniques (1998)
    Springer
    Euphytica 104 (1998), S. 143-149 
    Details
    ISSN: 1573-5060
    Schlagwort(e): potatoes ; Solanum tuberosum ; parent selection ; cross prediction ; breeding
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Cross prediction techniques were applied to data collected from over 600 hybrid combinations of potato (Solanum tuberosum) and analyzed to determine the potential of using early generation cross prediction techniques to identify superior parental clones. Performance of parental lines based on parameters collected in early generations were compared with the observed frequencies of desirable recombinants with a common parent in the latter stages of a breeding program. Results showed that value of parents, in their ability to produce desirable recombinants in breeding programs, can be predicted using univariate cross prediction techniques. This type of information can be available from early generation progeny trials and could easily be incorporated into a practical potato breeding scheme.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1018392430172
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