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Digitale Medien

Carboxin resistance transformation of the homobasidiomycete fungus Pleurotus ostreatus (2000)

Honda, Y. ; Matsuyama, T. ; Irie, T. ; [weitere]

Springer

Current genetics 37 (2000), S. 209-212

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Details

ISSN: 1432-0983

Schlagwort(e): Key words Basidiomycete ; Transformation ; White-rot fungi ; Carboxin ; Succinate:ubiquinone oxidoreductase

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie

Notizen: Abstract A novel selection marker gene for transformation of the white-rot basidiomycete Pleurotus ostreatus was developed by introducing a point mutation in a gene which encodes the iron-sulfur protein (Ip) subunit of succinate dehydrogenase. The mutant gene, Cbx R, encodes a modified Ip subunit with an amino-acid substitution (His239 to Leu) and confers resistance to the systemic fungicide, carboxin. The DNA sequence was integrated ectopically in the chromosome of the transformants. This is the first report of a homologous marker gene which is available for the molecular breeding of an edible mushroom.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/s002940050521

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