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  • Nosocomial infection  (1)
  • Thermus thermophilus  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Outbreaks of nosocomial rotavirus gastro-enteritis in a paediatric ward (1996)
    Springer
    European journal of pediatrics 155 (1996), S. 954-958 
    Details
    ISSN: 1432-1076
    Keywords: Nosocomial infection ; Rotavirus gastro-enteritis ; SDS-PAGE ; Serotyping ELISA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Faecal samples were collected from patients with gastro-enteritis during two winter seasons on a paediatric ward. Three outbreaks of nosocomial rotavirus gastro-enteritis were identified by latex agglutination and the virus strains were characterized by polyacrylamide gel electrophoresis of the genome nucleic acid and by subgrouping and serotyping enzyme-linked immunosorbent assays (ELISA). One outbreak was caused by serotype 1 rotavirus, one by serotype 2 and the remaining outbreak was caused by a mixture of serotypes 1 and 4. Identical electrophoretic patterns of the rotavirus genome in each outbreak combined with the ELISA results indicate that these three outbreaks were hospital-acquired cases. The index cases in the three outbreaks were community-acquired and one of two index cases in the second outbreak was hospital-acquired. On each occasion, susceptible roommates were easily infected from the index cases and then cross-infection occurred in the paediatric ward. Possible vehicles were the medical staff, especially doctors, parents of infected patients and infected patients who were moved to other rooms. One patient who had been treated with a series of antitumour therapies excreted rotaviruses in faeces for a long time period and probably played a role as a source.of the outbreak. Moreover, some patients still excreted rotaviruses in their normal stool 1 week after recovery from gastro-enteritis. These findings indicate that continual examination of stool samples for rotaviruses until they are negative may be important to prevent the spread of rotavirus infection.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02282886
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Studies on interdomain interaction of 3-isopropylmalate dehydrogenase from an extreme thermophile, Thermus thermophilus, by constructing chimeric enzymes (1999)
    Springer
    Extremophiles 3 (1999), S. 259-262 
    Details
    ISSN: 1433-4909
    Keywords: Key words Thermophilic enzymes ; 3-Isopropylmalate dehydrogenase ; Thermostability ; Thermus thermophilus ; Chimeric proteins ; Interdomain interaction ; Differential scanning calorimetry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In our previous study, we showed that a chimeric isopropylmalate dehydrogenase, 2T2M6T, between an extreme thermophile, Thermus thermophilus, and a mesophile, Bacillus subtilis, isopropylmalate dehydrogenases (the name roughly denotes the primary structure; the first 20% from the N-terminal is coded by the thermophile leuB gene, next 20% by mesophile, and the rest by the thermophile gene) denatured in two steps with a stable intermediate, suggesting that in the chimera some of the interdomain interaction was lost by amino acid substitutions in the "2M" part. To identify the residues involved in the interdomain interactions, the first and the second halves of the 2M part of the chimera were substituted with the corresponding sequence of the thermophile enzyme. Both chimeras, 3T1M6T and 2T1M7T, apparently showed one transition in the thermal denaturation without any stable intermediate state, suggesting that the cooperativity of the conformational stability was at least partly restored by the substitutions. The present study also suggested involvement of one or more basic residues in the unusual stability of the thermophile enzyme.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s007920050125
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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