ISSN:
1615-6102
Keywords:
Cytoskeleton
;
Triton X-100
;
Dry cleaving
;
Euglena gracilis
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary A three-dimensional network of structural filaments was visible with common electron microscopes in the cytoplasm ofEuglena gracilis green cells extracted with buffers containing the nonionic detergent Triton X-100. A similar filamentous web was detected at the periphery of critical point dried cells cleaved on grids by means of an adhesive tape. SDS-polyacrylamide gel electrophoresis of the detergent-resistent cytoskeleton showed that actin or actin-like proteins of molecular weight in the range of 43–45 K are not among the components having a structural role inEuglena. The significance of these findings was discussed in relation to the capability of the alga to change the cell shape.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01276344
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