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  • 1
    ISSN: 1432-0533
    Keywords: Key words GM1-gangliosidosis ; Ultrastructure ; Electrospray ionisation tandem mass spectrometry ; Diagnosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The post-mortem diagnosis of lysosomal storage diseases can be confounded by the unavailability of suitable material. Here we report the diagnosis of GM1-gangliosidosis in a cross-bred dog, from which only formalin-fixed brain was available, by a combination of electron microscopy and the detection of elevated levels of GM1-ganglioside within the tissue using the novel technique of electrospray ionisation tandem mass spectrometry. Electron microscopic examination of ultrathin sections of resin-embedded tissue revealed cytoplasmic inclusions (membranous cytoplasmic and zebra bodies) in brain stem and cerebellar neurons that were characteristic of a gangliosidosis. Glycolipids were extracted from the fixed tissue and analysed by tandem mass spectrometry. Two major ions were detected, which corresponded to GM1 (d18:1–C18:0) and GM1 (d20:1–C18:0). Their identity was confirmed by comparison of their fragmentation patterns with those of authentic standards. The concentration of GM1 was approximately sixfold higher on a wet weight basis than in the brain of a normal control dog, confirming the diagnosis of GM1-gangliosidosis.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-0832
    Keywords: Agriculture ; Biocontrol ; Residue ; Saprophytes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Thirteen species of fleshy fungi were identified from two agricultural fields during the 1989 and 1990 growing seasons at the Rural Development Center (RDC) and Horticulture Farm, Coastal Plain Experiment Station, Tifton, Georgia. The three most common fungi wereCoprinus plicatilis, Cyathus olla, andPanaeolina foenisecii. At the former site, residue management practices were established for evaluating crop production and at the Horticulture Farm different crops were grown with conservation tillage employing a Low Input Sustainable Vegetable Production system. Tillage treatments at the RDC were no-till, row-till, ridge-plant, and moldboard-plowing. Residue management of triticale included burning or cutting the stubble at a height of 20 or 60 cm at harvest. At the Horticulture Farm, seven winter cover crops were compared to a fallow control. No significant differences were shown between observations of fungal species and individual treatments at the Horticulture Farm during 1989 and 1990. However, there were treatment differences for observed fungi at the RDC farm on specific dates for both years. Furthermore, no individual treatment(s) during the investigation increased observation rates of specific fungal species. Non-burned debris treatments showed consistently higher populations and diversity of fungi than burn debris plots.
    Type of Medium: Electronic Resource
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