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  • 1
    Electronic Resource
    Electronic Resource
    Die Ausbreitung von Fluorescenzfarbstoffen in der Hamsterbackentasche (1968)
    Springer
    Clinical and experimental medicine 148 (1968), S. 72-80 
    Details
    ISSN: 1591-9528
    Keywords: Vital microscopy ; Fluorescence microscopy ; Capillary permeability ; Interstitial fluid ; Hamster cheek pouch ; Vitalmikroskopie ; Fluorescenzmikroskopie ; Gefäßpermeabilität ; Interstitielle Flüssigkeit ; Hamsterbackentasche
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Durch die lokale Injektion von Fluorescenzfarbstoffen in das lockere Bindegewebe der hervorgestülpten Hamsterbackentasche wurden Ausbreitung und Abtransport im Gewebe vitalmikroskopisch untersucht. Das frei wasserlösliche Fluorescein breitet sich in Gestalt perivasaler Streifen schnell in Richtung zur Basis der Backentasche hin aus, ohne dabei in das Blut überzutreten. Lymphgefäße gelangen nicht zur Darstellung. Das sich in vivo an Protein bindende Brillantsulfoflavin zeigt den streifenförmigen Abstrom verzögert und spärlich. Das in vitro an Plasmaproteine fest gekoppelte Fluorescein-Isothiocyanat bleibt während einer Beobachtungszeit von mehr als 30 min ohne Ausbreitung und Abstrom im Gewebe liegen. Der Stofftransport wasserlöslicher Substanzen im interstitiellen Gewebe der Hamsterbackentasche ist somit gerichtet und perivasal lokalisiert. Ein Abstrom über Lymphgefäße fehlt. Plasmaproteine bleiben im extravasalen Raum lange Zeit liegen.
    Notes: Summary The spread and flow of fluorescent dyes injected into the wall of the hamster cheek pouch was examined by vital microscopic means. Water-soluble fluorescein spreads very quickly in the form of perivascular streaks in the direction of the base of the cheek pouch, without entering into the blood stream. Lymph vessels, however, could not be identified. Brillant-sulfoflavine which binds proteinin vivo showed much slower and reduced flow phenomena. Fluorescein isothiocyanate coupled to plasma proteinsin vitro remained, during an observation period of more than 30 min, stationary in the cheek pouch tissue without any evidence of transport to be seen. The transport of water-soluble substances in the cheek pouch tissue is thus perivascular in nature. Lymph flow is not to be documented by this technique. This is further confirmed by the observation that fluorescent-labelled plasma proteins remain unmoved in the extravascular space of the cheek pouch tissue for a long period of time.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02044627
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  • 2
    Electronic Resource
    Electronic Resource
    Cytologische Befunde an Transplantattumoren in der Hamsterbackentasche (1972)
    Springer
    Research in experimental medicine 157 (1972), S. 47-60 
    Details
    ISSN: 1433-8580
    Keywords: Ultraviolet microscopy ; Hamster cheek pouch ; Tumor transplantation ; Vital microscopy ; Cell viability ; Ultraviolettmikroskopie ; Hamsterbackentasche ; Tumorüberpflanzung ; Vitalmikroskopie ; Zellvitalität
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Als Merkmale der Tumorbiologie wurden an homologen und heterologen Transplantattumoren in der Hamsterbackentasche die Tumorzellvitalität und die vitalmikroskopische Cytomorphologie untersucht. Es handelte sich um das amelanotische Hamstermelanom A.Mel. Nr. 3 und die menschlichen Coloncarcinome GW-39 und GW-77. Die mit einem Farbstofftest geprüfte Vitalität von frisch hergestellten Tumorzellsuspensionen erwies sich bei den Humantumoren als konstant hoch. Bei dem schnell wachsenden Hamstermelanom durchlief sie 1 Woche nach der Transplantation ein Maximum. Die zu verschiedenen Zeiten nach der Überpflanzung vorgenommenen mikroskopischen Untersuchungen der Feinstruktur unfixierter Tumorzellen verwendeten neben der Phasenkontrasttechnik vor allem die monochromatische Ultraviolettabsorption der Nucleinsäuren und Proteine. Die 3 Tumorzellarten zeigten strukturelle Besonderheiten, die als Grundlage für vergleichende Studien nach cytostatischer Therapie dienen können. Die Aussagemöglichkeiten der Vitalmikroskopie werden durch die Kombination der benutzten Methoden erweitert.
    Notes: Summary Viability and cytomorphology of unfixed cells from 3 transplantable tumors growing in the cheek pouch of unconditioned golden hamsters were studied as a tool of tumor biology. The amelanotic hamster melanoma A.Mel. No. 3 and the human colonic tumors GW-39 and GW-77 were used. Tumor cell suspensions of the xenogeneic tumors are constantly viable in a high percentage, as prouven by a dye exclusion test. The viability of the fast growing hamster melanoma reaches a maximum 1 week after transplantation. — The cellular structures were studied by means of phase contrast microscopy as well as by monochromatic absorptions of nucleic acids and proteins in the ultraviolet microscope. Structural differences of the 3 tumor cell types could be distinguished, suited as a basis for chemotherapeutic studies. Thus, the methods used are well suited to enlarge the diagnostic value of vital microscopy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01851397
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Microsatellite Variation in Two Populations of Free-Ranging Yellow Baboons (Papio hamadryas cynocephalus) (1998)
    Springer
    International journal of primatology 19 (1998), S. 273-285 
    Details
    ISSN: 1573-8604
    Keywords: Papio ; microsatellite loci ; DNA polymorphism ; population structure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We investigated genetic variation at six microsatellite (simple sequence repeat) loci in yellow baboons (Papio hamadryas cynocephalus) at two localities: the Tana River Primate Reserve in eastern Kenya and Mikumi National Park, central Tanzania. The six loci (D1S158, D2S144, D4S243, D5S1466, D16S508, and D17S804) were all originally cloned from and characterized in the human genome. These microsatellites are polymorphic in both baboon populations, with the average heterozygosity across loci equal to 0.731 in the Tana River sample and 0.787 in the Mikumi sample. The genetic differentiation between the two populations is substantial. Kolmogornov–Smirnov tests indicate that five of the six loci are significantly different in allele frequencies in the two populations. The mean F ST across loci is 0.069, and Shriver's measure of genetic distance, which was developed for microsatellite loci (Shriver et al., 1995), is 0.255. This genetic distance is larger than corresponding distances among human populations residing in different continents. We conclude that (a) the arrays of alleles present at these six microsatellite loci in two geographically separated populations of yellow baboons are quite similar, but (b) the two populations exhibit significant differences in allele frequencies. This study illustrates the potential value of human microsatellite loci for analyses of population genetic structure in baboons and suggests that this approach will be useful in studies of other Old World monkeys.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1020331615944
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    Paper (German National Licenses)
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