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  • Analytical Chemistry and Spectroscopy  (5)
  • 27.60.+j  (4)
  • cholesterol  (3)
  • 1
    Electronic Resource
    Electronic Resource
    Alterations in apolipoprotein B-48 in the postprandial state in NIDDM (1994)
    Springer
    Diabetologia 37 (1994), S. 1259-1264 
    Details
    ISSN: 1432-0428
    Keywords: Apolipoprotein B-48 ; triglyceride-rich lipoproteins ; NIDDM ; cholesterol ; triglyceride
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The intestine is a major site of cholesterol synthesis and produces apolipoprotein B-48, which is critical for intestinal cholesterol absorption and secretion. The purpose of this study was to examine postprandial changes in apolipoprotein B-48 in diabetes. Six non-insulin-dependent diabetic patients and six non-diabetic control subjects were given a high-fat meal (1300 kcal) and blood samples were taken pre- and postprandially, from which the triglyceride-rich lipoprotein fraction was isolated by ultracentrifugation (density〈1.006 g/ml). Apolipoprotein B-48 was separated on 4–15% gradient gels and quantified as a percentage of the fasting concentration by densitometric scanning. Total protein, triglyceride and cholesterol in the triglyceride-rich lipoprotein fraction, blood glucose, and serum insulin were also measured. Diabetic patients exhibited a postprandial triglyceride-rich apolipoprotein B-48 profile significantly different from that of control subjects (p〈0.05). The triglyceride and total protein concentration in the triglyceride-rich lipoprotein fraction mirrored the post-prandial profile and apolipoprotein B-48 in both groups. Significantly different patterns for triglyceride (p〈0.02) and total protein (p〈0.05) following the fat-rich meal were observed in the two groups. Fasting and postprandial triglyceride-rich lipoprotein cholesterol and total apolipoprotein B were significantly higher in diabetic patients than in control subjects (p〈0.05). Since apolipoprotein B-48 is the structural protein of intestinally-derived lipoprotein particles, these studies suggest an abnormality in intestinal lipoprotein metabolism in diabetes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00399800
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  • 2
    Electronic Resource
    Electronic Resource
    Alterations in apolipoprotein B-48 in the postprandial state in NIDDM (1994)
    Springer
    Diabetologia 37 (1994), S. 1259-1264 
    Details
    ISSN: 1432-0428
    Keywords: Key words Apolipoprotein B-48 ; triglyceride-rich lipoproteins ; NIDDM ; cholesterol ; triglyceride.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The intestine is a major site of cholesterol synthesis and produces apolipoprotein B-48, which is critical for intestinal cholesterol absorption and secretion. The purpose of this study was to examine postprandial changes in apolipoprotein B-48 in diabetes. Six non-insulin-dependent diabetic patients and six non-diabetic control subjects were given a high-fat meal (1300 kcal) and blood samples were taken pre- and postprandially, from which the triglyceride-rich lipoprotein fraction was isolated by ultracentrifugation (density 〈 1.006 g/ml). Apolipoprotein B-48 was separated on 4–15 % gradient gels and quantified as a percentage of the fasting concentration by densitometric scanning. Total protein, triglyceride and cholesterol in the triglyceride-rich lipoprotein fraction, blood glucose, and serum insulin were also measured. Diabetic patients exhibited a postprandial triglyceride-rich apolipoprotein B-48 profile significantly different from that of control subjects (p 〈 0.05). The triglyceride and total protein concentration in the triglyceride-rich lipoprotein fraction mirrored the post-prandial profile and apolipoprotein B-48 in both groups. Significantly different patterns for triglyceride (p 〈 0.02) and total protein (p 〈 0.05) following the fat-rich meal were observed in the two groups. Fasting and postprandial triglyceride-rich lipoprotein cholesterol and total apolipoprotein B were significantly higher in diabetic patients than in control subjects (p 〈 0.05). Since apolipoprotein B-48 is the structural protein of intestinally-derived lipoprotein particles, these studies suggest an abnormality in intestinal lipoprotein metabolism in diabetes. [Diabetologia (1994) 37: 1259–1264]
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00399800
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  • 3
    Electronic Resource
    Electronic Resource
    Cellular cholesterol regulation — A defect in the Type 2 (non-insulin-dependent) diabetic patient in poor metabolic control (1990)
    Springer
    Diabetologia 33 (1990), S. 93-99 
    Details
    ISSN: 1432-0428
    Keywords: Type 2 (non-insulin-dependent) diabetes mellitus ; LDL ; cholesterol ; esterification ; glycosylation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary This study investigates the relationship between Type 2 (non-insulin-dependent) diabetes mellitus and hypercholesterolaemia with regard to delivery of cholesterol to cells and regulation of endogenous cholesterol synthesis. The ability of LDL, from hypercholesterolaemic and Type 2 diabetic patients, to suppress cellular cholesterologenesis and to enhance mitogen-stimulated lymphocyte proliferation was compared. Cholesterol synthesis was estimated by measuring [14C]-acetate incorporation into cholesterol and lymphocyte proliferation was assessed by [3H]-thymidine incorporation into mitogen-stimulated normal lymphocytes. The results indicate that LDL from both Type 2 diabetic patients in poor metabolic control and hypercholesterolaemic patients was significantly less effective (p 〈 0.001) than LDL from non-diabetic normocholesterolaemic subjects in suppressing cholesterol synthesis in lymphocytes. LDL from all hypercholesterolaemic patients enhanced lymphocyte proliferation to a greater extent than LDL from normocholesterolaemic subjects and this effect was significantly increased using LDL from Type 2 diabetic, hypercholesterolaemic patients. Both suppression of [14C]-acetate incorporation and enhancement of [3H]-thymidine uptake could be related to an increased esterified/free cholesterol ratio in the LDL particle. The fact that cholesterol synthesis and cell proliferation were markedly altered by the above changes in LDL composition suggests a mechanism for cellular cholesterol accumulation in the Type 2 diabetic patient, even in the absence of elevated serum cholesterol levels.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00401046
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  • 4
    Electronic Resource
    Electronic Resource
    Identification and spectroscopy of the108Te nucleus (1994)
    Springer
    The European physical journal 350 (1994), S. 3-4 
    Details
    ISSN: 1434-601X
    Keywords: 23.20.Lv ; 25.70.Gh ; 27.60.+j
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Excited states of 108 Te were populated in the 54 Fe + 58 Ni (270 MeV) reaction using the NORDBALL detector array equipped with charged-particle and neutron detector systems for reaction channel separation. Gamma rays belonging to the neutron deficient nucleus 108 Te were identified for the first time. On the basis of γγ-coincidence and angular correlation relations a level scheme was constructed with tentative spin and parity assignments. The structure of the nucleus is discussed in the framework of systematics of heavier Te nuclei.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01285042
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  • 5
    Electronic Resource
    Electronic Resource
    Excited states of111I and the observation of a 21 ns isomer (1994)
    Springer
    The European physical journal 350 (1994), S. 179-180 
    Details
    ISSN: 1434-601X
    Keywords: 23.20.Lv ; 25.70.Gh ; 27.60.+j
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Proton rich nuclei close to 100 Sn have been investigated inbeam using the NORDBALL detector array. A beam of 270 MeV58 Ni was used to bombard a 54 Fe target. Reaction channel separation was achieved with a 4π charged particle multi-detector set-up together with a 1π neutron detector wall placed in the forward direction. Gamma-ray transitions belonging to the 111 I nucleus were identified for the first time. A level scheme constructed from γ-γ-particle-coincidence analysis is proposed. The level structure is discussed within the framework of the shell model and the systematics of the heavier odd iodine nuclei.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01289577
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  • 6
    Electronic Resource
    Electronic Resource
    Shape coexistence in117I (1992)
    Springer
    The European physical journal 344 (1992), S. 223-224 
    Details
    ISSN: 1434-601X
    Keywords: numbers ; 25.70.−z ; 27.60.+j
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The 117Inucleus has been investigated in a high-spin γ-spectroscopic study using the NORDBALL detector array. The observed level structures are interpreted as resulting from coexisting collective prolate and oblate as well as non-collective oblate shapes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01291708
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  • 7
    Electronic Resource
    Electronic Resource
    In-beamγ-ray spectroscopy of102In (1993)
    Springer
    The European physical journal 345 (1993), S. 243-244 
    Details
    ISSN: 1434-601X
    Keywords: 23.20.Lv ; 25.70.Gh ; 27.60.+j
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Neutron deficient nuclei close to 100Snhave been investigated in-beam by γ-ray spectroscopic methods using the NORDBALL detector array. A beam of 270 MeV 58Niwas used to bombard a target of 54Fe.Reaction channel separation was achieved with a 4π charged particle multidetector setup together with a 1π neutron detector wall placed in the forward direction. Excited states of 102Inwere identified for the first time. The level scheme constructed from γ-γ-particle-coincidence and γ-γ-angular correlation analysis is presented. The structure of 102 In is discussed and compared to neighboring nuclei in the framework of the nuclear shell-model.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01293351
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  • 8
    Electronic Resource
    Electronic Resource
    Raman spectroscopic determination of the dissociation constants of an acetonitrile-zinc complex in aqueous solution and the enthalpy and entropy of complex dissociation (1991)
    Chichester [u.a.] : Wiley-Blackwell
    Journal of Raman Spectroscopy 22 (1991), S. 651-656 
    Details
    ISSN: 0377-0486
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: The dissociation constants for an acetonitrile-zinc complex, (CH3CN)Zn2+, were measured in aqueous solutions containing zinc salts (40-80%, w/w) and acetonitrile by Raman spectroscopy. Quantitative measurements of the intensity of the free and complexed ν(CN) Raman bands at 2264 and 2320 cm-1, respectively, were used to determine the average enthalpy and entropy of the complex dissociation to free acetonitrile in the temperature range 293-343 K for a 70% (w/w) solution of zinc chloride (0.052 mol) in an aqueous solution containing acetonitrile (0.024 mol); δH° and δS° values of 8.4 ± 0.5 kJ mol-1, respectively, were determined.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jrs.1250221110
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  • 9
    Electronic Resource
    Electronic Resource
    Fourier transform Raman spectroscopic study of hydrogen bonding in 2,5-dimercapto-1,3,4-thiadiazole using an environmental chamberThis paper is dedicated to Professor D. A. Long on the occasion of his 70th birthday. (1995)
    Chichester [u.a.] : Wiley-Blackwell
    Journal of Raman Spectroscopy 26 (1995), S. 617-622 
    Details
    ISSN: 0377-0486
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: The bands at 2485 and 940 cm-1 observed in the Raman spectra of 2,5-dimercapto-1,3,4-thiadiazole represent the hydrogen-bonded ν(SH) stretching and δ(C-SH) in-plane bending modes, respectively. A quantitative study of the hydrogen bonding was carried out using intensity measurements of the bands assigned to the hydrogen-bonded and the free δ(C-SH) in-plane deformations at 940 and 919 cm-1, respectively, as a function of temperature. The Fourier transform (FT) Raman spectra were recorded over the temperature range 303-403 K using an environmental chamber fitted into the FT Raman sample compartment. The equilibrium constants between the free and the hydrogen-bonded molecules were determined over this temperature range and the average enthalpy for hydrogen-bond formation was obtained (ΔH° = -3.35 ± 0.2 kJ mol-1).
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jrs.1250260806
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  • 10
    Electronic Resource
    Electronic Resource
    Applications of Raman spectroscopy to the study of polymers and polymerization processes (1993)
    Chichester [u.a.] : Wiley-Blackwell
    Journal of Raman Spectroscopy 24 (1993), S. 475-483 
    Details
    ISSN: 0377-0486
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: The application of Raman spectroscopy to polymers and polymerization processes is surveyed and the advent of recent advances in instrumental techniques to polymer analysis is highlighted. Some of the problems of the more widely-used infrared spectroscopic methods, for example in microstructural analysis of polydienes and copolymers, are discussed and the advantages of the Raman spectroscopic technique are explained. The use of near-infrared excitation and Fourier-transform Raman instrumentation for process monitoring and polymer composite studies is discussed. Raman microscopic and waveguide spectroscopic studies of small samples and polymer coatings on substrates are considered. Other applications are mentioned in the areas of polymer orientation, surface-enhanced Raman scattering, conducting polymers, biopolymers and time-resolved studies.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jrs.1250240803
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