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  • 1
    Electronic Resource
    Electronic Resource
    Identification of wheat-Agropyron cristatum monosomic addition lines by RFLP analysis using a set of assigned wheat DNA probes (1994)
    Springer
    Theoretical and applied genetics 89 (1994), S. 70-75 
    Details
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; Agropyron cristatum ; Alien addition ; RFLP ; Non-radioactive labelling
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A non-radioactive digoxigenin-labelled DNA method was used successfully to identify RFLP markers in 54 Triticum aestivum cv ‘Chinese Spring’ — Agropyron cristatum (2n=28, genome PPPP) P-genome monosomic addition lines. Southern analysis using a set of 14 DNA probes identifying each homoeologous chromosome arm, combined with two restriction enzymes HindIII and EcoRI, indicated that six A. cristatum chromosomes (1P, 2P, 3P, 4P, 5P and 6P) and five A. cristatum chromosome arms (2PS, 2PL, 5PL, 6PS and 6PL) have been individually added to the wheat genome. The added chromosomes of three lines were Agropyron translocated chromosomes. It was also found that two addition plants possessed an Agropyron-wheat translocation. These results showed that RFLP analysis using the set of assigned wheat probes was a powerful tool in detecting and establishing homoeology of alien A. cristatum chromosomes, or arms, added to wheat, as well as in screening the alien addition material. The creation of the monosomic addition lines should be useful for the transfer of disease-resistance genes from A. cristatum to wheat.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00226985
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  • 2
    Electronic Resource
    Electronic Resource
    Measurements of hydrogen peroxide induced premature senescence:senescence-associated β-galactosidase and DNA synthesis index in human diploid fibroblasts with down-regulated p53 or Rb (2000)
    Springer
    Biogerontology 1 (2000), S. 335-339 
    Details
    ISSN: 1573-6768
    Keywords: dose response ; growth arrest ; oxidants ; senescent biomarkers
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Human diploid fibroblasts (HDFs) inevitably undergo replicative senescence in culture after serial passages. Recent work indicates that early passage HDFs undergo irreversible growth arrest and develop features of senescence after being treated with oxidants and other agents. Senescence is usually measured by a decrease in DNA synthesis index and an increase in the activity of senescence-associated β-galactosidase (SA β-gal). We compared these two measurements here and found that IMR-90 HDFs lost the ability to synthesize DNA immediately but did not activate SA β-gal until 4 days after the treatment with 75 µM or 0.75 pmol/cell H2O2. Expression of human papillomaviral E6 or/and E7 genes results in reduction of p53 or/and Rb protein levels and increases in ED50 for DNA synthesis inhibition or SA β-gal expression. A small fraction of wild type and E7 expressing cells could not synthesize DNA and did not express SA β-gal one week following the treatment with H2O2 at doses lower than 150 µM or 1.5 pmol/cell. The dose response curve of SA β-gal activation overlapped with that of DNA synthesis inhibition in E6 and E6E7 expressing cells. The results indicate that the expression of SA β-gal correlates with inability of DNA synthesis in the majority of wild type, E6, E7 or E6E7 expressing cells one week after H2O2 treatment.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1026590501344
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    Paper (German National Licenses)
    Fulltext
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