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  • 1
    Electronic Resource
    Electronic Resource
    Impairment of Intestinal Mucosal Antioxidant Defense System During Salmonella typhimurium Infection (1998)
    Springer
    Digestive diseases and sciences 43 (1998), S. 646-651 
    Details
    ISSN: 1573-2568
    Keywords: ANTIOXIDANTS ; INTESTINE ; SALMONELLA TYPHIMURIUM GLUTATHIONE
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The mucosal pathology of Salmonella typhimuriuminfection may in part be due to the excessive productionof reactive oxygen species (ROS). The influence of S.typhimurium infection on the intestinal mucosal antioxidant defense system was investigated. Weinjected ligated rat ileal loops with Salmonella liveculture or toxin. After 18 hr of infection, the animalswere killed and enterocytes isolated from the ileal loops. The enterocyte-reduced glutathione(GSH) content and activities of the enzymes superoxidedismutase (SOD), glutathione peroxidase (GSH-Px),catalase, glutathione-S-transferase (GST), glutathione reductase (GR), and glucose-6-phosphatedehydrogenase (G6PDH) were spectrophotometricallyestimated. The vitamin E and A contents were determinedby high-performance liquid chromatography (HPLC). Inboth the Salmonella live culture and toxin-treatedgroups, the enterocyte GSH and vitamin E contents andactivities of the enzymes SOD, GSH-Px, catalase, GR, andG6PDH were significantly decreased as compared to the control group. However there was asignificant increase in the enterocyte activity of GST.There was no change in the vitamin A content of theenterocytes. These findings might indicate a decreased endogenous intestinal protection against ROS inS. typhimurium-mediated infection, which couldcontribute to the pathogenesis of the disease.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018887813713
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  • 2
    Electronic Resource
    Electronic Resource
    Intestinal mucosal lipid peroxidation and absorptive function In Salmonella typhimurium mediated intestinal infection (1998)
    Springer
    Molecular and cellular biochemistry 178 (1998), S. 345-352 
    Details
    ISSN: 1573-4919
    Keywords: Salmonella typhimurium ; brush border membrane ; lipid peroxidation ; free radicals ; antioxidants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract S. typhimurium infection is associated with neutrophil infiltration within the intestinal mucosa. Neutrophil activation provides a major source of reactive oxygen species (ROS). The mucosal pathology of S. typhimurium infection may be in part due to the excessive production of these reactive species. This study was carried out to investigate if ROS play a role in mediating the changes in the structural components and functional properties of brush border membrane (BBM) in rats during S. typhimurium infection. This was done by determining the changes in the BBM extent of lipid peroxidation and absorptive function. A significant increase in the extent of lipid peroxidation of BBM during S. typhimurium infection was observed as judged by malondialdehyde (MDA) and conjugated diene formation and depletion of α-tocopherol and protein associated thiol groups. A significant decrease in the BBMV (brush border membrane vesicle) transport of amino acids was also observed. However there was no change in the transport of D-glucose. The decrease in amino acid transport further led to a significant decrease in the enterocyte level of protein synthesis. Exposure of BBMV to a free radical donor, cumene hydroperoxide, also led to an increase in the extent of lipid peroxidation and a decrease in the amino acid transport. Possibly ROS might play a significant role in mediating the mucosal damage during S. typhimurium infection.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006891019115
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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