ISSN:
1617-4623
Keywords:
gypsy-like retrotransposon
;
Nicotiana alata
;
S-locus
;
Touch-inducible
;
Pollination
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract We have identified a family of repetitive sequences in the genome ofNicotiana alata namedTnal (Transposon ofN. alata). The first element we characterised was on a genomic clone for theN. alata S6-ribonuclease (S6-RNase), a gene required for self-incompatibility in this species. The DNA sequence of this element resembles the integrase domain of retro-transposons of thegypsy class and is most similar to a retrotransposon fromLilium henryi. A transcript present inN. alata styles (self-incompatibility genotype S6S6) hybridized toTnal and accumulated in the style following either pollination or touching. This transcript was cloned from a cDNA library and was encoded by a second, partialTnal element. Neither the transcribed sequence nor the originalTnal element contain an open reading frame or is likely to be able to transpose. The second element was mapped using a population ofN. alata plants segregating for alleles of the self-incompatibility locus and is closely linked to the S6-allele. TheTnal element is present in a number ofNicotiana species and appears to have been active at least twice during the evolution of this genus.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02174177
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