ISSN:
1573-1561
Keywords:
Condensed tannins
;
catechin
;
vanillin-HCl method
;
organic solvents
;
methanol
;
acetone
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Abstract Tannins are generally extracted using aqueous organic solvents, mainly methanol and acetone. The presence of various concentrations of methanol in the sample containing catechin or tannins did not alter the kinetics of their reaction, but the absorbance depended on the amount of methanol in the sample; the higher the methanol, the higher was the absorbance. Acetone reacted with acidified vanillin to produce a chromogen with λmax at 548 nm, which produce a substantial error in the determination of condensed tannins. In the presence of acetone, the time courses of the reaction for catechin and tannins were different, which depended on the acetone concentration and the reaction temperature. Reaction conditions for catechin and tannins that enable their measurement in the presence of acetone are presented.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00984996
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