ISSN:
1573-4994
Keywords:
Fluorescence
;
multiplex dyes
;
photochemistry
;
time-resolved spectroscopy
Source:
Springer Online Journal Archives 1860-2000
Topics:
Physics
Notes:
Abstract New dyes with characteristic fluorescence lifetimes have been developed for bioanalytical applications. Based upon the concept of “multiplex dyes,” we have designed rhodamine dyes with nearly identical absorption and emission spectral characteristics but different fluorescence lifetimes. Extending this principle to applications with laser diodes, new rhodamines with functional groups for covalent coupling of analytes have been developed. The new labels exhibit absortion and fluorescence beyond 600 nm and have a high quantum efficiency, even in aqueous buffer systems.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00862730
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