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  • 1
    Electronic Resource
    Electronic Resource
    Developing a transposon tagging system to isolate rust-resistance genes from flax (1992)
    Springer
    Theoretical and applied genetics 85 (1992), S. 46-54 
    Details
    ISSN: 1432-2242
    Keywords: Linum usitatissimum ; Melampsora lini ; Rust-resistance genes ; Ac/Ds gene tagging
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A line of flax, homozygous for four genes controlling resistance to flax rust, was transformed with T-DNA vectors carrying the maize transposable elements Ac and Ds to assess whether transposition frequency would be high enough to allow transposon tagging of the resistance genes. Transposition was much less frequent in flax than in Solanaceous hosts such as tobacco, tomato and potato. Transposition frequency in callus tissue, but not in plants, was increased by modifications to the transposase gene of Ac. Transactivation of the excision of a Ds element was achieved by expressing a cDNA copy of the Ac transposase gene from the Agrobacterium T-DNA 2′ promoter. Progeny of three plants transformed with Ac and 15 plants transformed with Ds and the transposase gene, were examined for transposition occurring in the absence of selection. Transposition was observed in the descendants of only one plant which contained at least nine copies of Ac. Newly transposed Ac elements were observed in 25–30% of the progeny of some members of this family and one active Ac element was located 28.8 (SE=6.3) map units from the L 6 rust-resistance gene. This family will be potentially useful in our resistance gene tagging program.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00223843
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Hydrocarbon accumulation in freshwater sediments of an urban catchment (1982)
    Springer
    Hydrobiologia 91-92 (1982), S. 285-292 
    Details
    ISSN: 1573-5117
    Keywords: PAH ; aliphatic hydrocarbons ; sediment ; stormwater
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The top and bottom of two sediment cores collected from an urban receiving basin in NW London, and stormwater samples from the attendant catchment, have been analysed for their hydrocarbon content. In surface sediments, basal sediments and stormwater, total aliphatic hydrocarbon levels are 445–690 µg g−1 dry wt., 43–224 µg g−1 and 0.36–1.10 mg l−1, respectively; and total levels of polyaromatic hydrocarbons (PAHs) are 780–1 100 µg g−1, 310–640 µg g1 and 5.83–18.21 mg l−1, respectively. Biodegradation of aliphatics is assessed by phytane:n-C18 and pristane: n-C17 ratios. Hydrocarbon sources are determined from phytane: pristane ratios, odd: even carbon chain length ratios, the presence of an unresolved complex mixture, and by comparison of the amount of methyl-substituted PAH s with that of the parent compounds. Comparison of total levels between surface and basal sediments shows a 1 to 3 fold increase in total PAHs and a 3 to 10 fold increase in aliphatic hydrocarbons over a 120 year period.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02391945
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Hydrocarbon accumulation in freshwater sediments of an urban catchment (1982)
    Springer
    Hydrobiologia 91-92 (1982), S. 285-292 
    Details
    ISSN: 1573-5117
    Keywords: PAH ; aliphatic hydrocarbons ; sediment ; stormwater
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The top and bottom of two sediment cores collected from an urban receiving basin in NW London, and stormwater samples from the attendant catchment, have been analysed for their hydrocarbon content. In surface sediments, basal sediments and stormwater, total aliphatic hydrocarbon levels are 445–690 µg g−1 dry wt., 43–224 µg g−1 and 0.36–1.10 mg l−1, respectively; and total levels of polyaromatic hydrocarbons (PAHs) are 780–1 100 µg g−1, 310–640 µg g1 and 5.83–18.21 mg l−1, respectively. Biodegradation of aliphatics is assessed by phytane:n-C18 and pristane: n-C17 ratios. Hydrocarbon sources are determined from phytane: pristane ratios, odd: even carbon chain length ratios, the presence of an unresolved complex mixture, and by comparison of the amount of methyl-substituted PAH s with that of the parent compounds. Comparison of total levels between surface and basal sediments shows a 1 to 3 fold increase in total PAHs and a 3 to 10 fold increase in aliphatic hydrocarbons over a 120 year period.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00940119
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
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