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  • 1
    ISSN: 1460-9568
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Medizin
    Notizen: Vertebrate synapsins are abundant synaptic vesicle phosphoproteins that have been proposed to fine-regulate neurotransmitter release by phosphorylation-dependent control of synaptic vesicle motility. However, the consequences of a total lack of all synapsin isoforms due to a knock-out of all three mouse synapsin genes have not yet been investigated. In Drosophila a single synapsin gene encodes several isoforms and is expressed in most synaptic terminals. Thus the targeted deletion of the synapsin gene of Drosophila eliminates the possibility of functional knock-out complementation by other isoforms. Unexpectedly, synapsin null mutant flies show no obvious defects in brain morphology, and no striking qualitative changes in behaviour are observed. Ultrastructural analysis of an identified ‘model’ synapse of the larval nerve muscle preparation revealed no difference between wild-type and mutant, and spontaneous or evoked excitatory junction potentials at this synapse were normal up to a stimulus frequency of 5 Hz. However, when several behavioural responses were analysed quantitatively, specific differences between mutant and wild-type flies are noted. Adult locomotor activity, optomotor responses at high pattern velocities, wing beat frequency, and visual pattern preference are modified. Synapsin mutant flies show faster habituation of an olfactory jump response, enhanced ethanol tolerance, and significant defects in learning and memory as measured using three different paradigms. Larval behavioural defects are described in a separate paper. We conclude that Drosophila synapsins play a significant role in nervous system function, which is subtle at the cellular level but manifests itself in complex behaviour.
    Materialart: Digitale Medien
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  • 2
    Digitale Medien
    Digitale Medien
    [s.l.] : Nature Publishing Group
    Nature 295 (1982), S. 405-407 
    ISSN: 1476-4687
    Quelle: Nature Archives 1869 - 2009
    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
    Notizen: [Auszug] As in most arthropods, the mushroom bodies of Drosophila are two conspicuous substructures of the median protocerebrum arranged in mirror symmetry to the saggital midplane. The numerous, relatively small cell bodies of their intrinsic neurones (Kenyon cells) are situated in the posterior dorsal ...
    Materialart: Digitale Medien
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  • 3
    Digitale Medien
    Digitale Medien
    Springer
    Journal of comparative physiology 80 (1972), S. 119-136 
    ISSN: 1432-1351
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Phototaxis and optomotor reactions of the mutantsebony and opm 2 are investigated. LikeMusca, Drosophila has two complementary visual input systems, one specialized (e. g.) in optimal contrast transfer (high acuity system, HAS, retinula cells 7 and 8) the other in high sensitivity (HSS, retinula cells 1–6). Inebony the HSS seems to be blocked for phototaxis and optomotor responses (Figs. 1 A, 5). However, even the HAS has a higher threshold intensity than in wild type (Fig. 5). In opm 2 the HSS is disturbed for phototaxis (Fig. 1) but is operating for the optomotor response (Fig. 6). However the HAS seems to be largely suppressed for the optomotor response (Table 2). In the double mutantebony-opm 2 both visual input systems seem to be impaired for movement detection (Fig. 7). Two other properties of the visual system of opm 2 are described. The visual fields of the sampling stations for movement detection are about twice as large as in wild type (Figs. 2A, B). This can not be explained by a disturbance of the optics. In other mutants of this type the visual field size is slightly different. In opm 2 the reaction to movement from front to back (progressive) is specificly suppressed, whereas, at least in flight, the reaction to movement from back to front (regressive) is normal (Table 1, Figs. 3, 4). The degree of suppression of the reaction to progressive movement is variable in individual flies and differs for the three mutants of the opm 2-group (Table 1). In a simple example the use of these mutants for system analysis experiments is demonstrated.
    Materialart: Digitale Medien
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  • 4
    Digitale Medien
    Digitale Medien
    Springer
    Journal of comparative physiology 98 (1975), S. 217-241 
    ISSN: 1432-1351
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Partially blind mutants can be used to investigate the processing of visual information in the fruit flyDrosophila. This approach requires (1) procedures for the selection of a variety of partially blind mutants, and (2) a strategy for the identification and coordination of visual malfunctions by comparison of interrelated traits of behaviour. The two selection techniques so far employed to recover partially blind mutants use either the fast phototaxis or the optomotor response as selection determining behaviour. The second method is described here and is applied specifically to select mutants in which one of the two autonomous subsystems of vision designated asHigh Sensitivity System andHigh Acuity System is defective. (The mutants obtained are apparently normal with respect to their HAS whereas the HSS is blocked.) Two sets of experiments have been developed in order to test interrelated traits of behaviour in a comparatively large number of flies. One set of experiments measuresslow phototaxis as a function of light intensity. The other is to determine the optomotor response to moving patterns of different spatial periods as functions of both the average brightness and the speed of the movement. Further techniques such as electroretinography and optical inspection of the eyes are used to complement the behavioural approach. By combination of the different tests a first step has been made in the characterization and classification of partially blind mutants with neuronal disorders obtained by different selection procedures and in different laboratories.
    Materialart: Digitale Medien
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  • 5
    Digitale Medien
    Digitale Medien
    Springer
    Journal of comparative physiology 145 (1982), S. 321-329 
    ISSN: 1432-1351
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The well known optomotor yaw torque response in flies is part of a 3-dimensional system. Optomotor responses around the longitudinal and transversal body axes (roll and pitch) with strinkingly similar properties to the optomotor yaw response are described here forDrosophila melanogaster. Stimulated by visual motion from a striped drum rotating around an axis aligned with the measuring axis, a fly responds with torque of the same polarity as that of the rotation of the pattern. In this stimulus situation the optomotor responses for yaw, pitch and roll torque have about the same amplitudes and dynamic properties (Fig. 2). Pronounced negative responses are measured with periodic gratings of low pattern wavelengths due to geometrical interference (Fig. 3). The responses depend upon the contrast frequency rather than the angular velocity of the pattern (Fig. 4). Like the optomotor yaw response, roll and pitch responses can be elicited by small field motion in most parts of the visual field; only for motion below and behind the fly roll and pitch responses have low sensitivity. The mutantoptomotor-blind H31 (omb H31) in which the giant neurones of the lobula plate are missing or severely reduced, is impaired in all 3 optomotor torque responses (Fig. 5) whereas other visual responses like the optomotor lift/thrust response and the landing response (elicited by horizontal front-to-back motion) are not affected (Heisenberg et al. 1978). We propose that the lobula plate giant neurons mediate optomotor torque responses and that the VS-cells in particular are involved in roll and pitch but not in lift/thrust control. This hypothesis accommodates various electrophysiological and anatomical observations about these neurons in large flies.
    Materialart: Digitale Medien
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  • 6
    Digitale Medien
    Digitale Medien
    Springer
    Journal of comparative physiology 147 (1982), S. 479-484 
    ISSN: 1432-1351
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Drosophila melanogaster is able to perform osmotropotaxis under open-loop conditions. With an ‘optimal’ stimulus the average turning tendency to the side of higher concentration corresponds to a circular track with radiusr=0.8 cm. The response amplitude does not decrease within 1 or 2 h. Unilaterally antennectomized flies in an homogeneous odor field show a permanent turning tendency towards their intact side. The smallest concentration ratio to elicit osmotropotaxis in normal flies ranges between 6∶10 and 9∶10 at high and between 2∶10 and 5∶10 at an about 50 times lower odor intensity. No negative tropotaxis (i.e. turning to the side of lower concentration) is observed, even with strong repellents.
    Materialart: Digitale Medien
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  • 7
    Digitale Medien
    Digitale Medien
    Springer
    Journal of comparative physiology 169 (1991), S. 699-705 
    ISSN: 1432-1351
    Schlagwort(e): Operant orientation behavior ; Initiating activity ; Learning by doing ; Sensory-motor correlation ; Drosophila melanogaster
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Operant behavior is studied in tethered Drosophila flies using visual motion, heat or odour as operandum and yaw torque, thrust or direction of flight as operans in various combinations (Fig. 1). On the basis of these results a conceptual framework of operant behavior is proposed: (1) It requires a goal (desired state) of which the actual state deviates. (2) To attain the goal a range of motor programs is activated (initiating activity, see Fig. 7). (3) Efference copies of the motor programs are compared to the sensory input referring to the deviation from the desired state (e.g. by cross-correlation). (4) In case of a significant coincidence the respective motor program is used to modify the sensory input in the direction towards the goal. (5) Consistent control of a sensory stimulus by a behavior may lead to a more permanent behavioral change (conditioning). In this scheme operant activity (1–4) and operant conditioning (1–5) are distinguished.
    Materialart: Digitale Medien
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  • 8
    Digitale Medien
    Digitale Medien
    Springer
    Journal of comparative physiology 117 (1977), S. 127-162 
    ISSN: 1432-1351
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary We propose that inDrosophila melanogaster the optomotor response to both horizontal and vertical movement is mediated predominantly by the 6 large retinula cells (R1–6) in each facet of the compound eye. Evidence is presented which indicates that this may also be true for most of the other visual responses which at present can be quantitatively studied. These responses include visually controlled landing, pattern-induced orientation of flying and walking animals, the abnormal jump reflex of the mutant Hk1 (Kaplan, 1976) and probably also phototaxis. The only function for which the small retinula cells R7 and/or R8 seem to be required so far is spectral wavelength discrimination in phototaxis at high light intensity. Our hypothesis is based on studies of the receptor deficient mutantssevenless, outer rhabdomeres absent andreceptor degeneration B as well as on results of bleaching experiments by which the retinula cells R1–6 of the eye color mutantwhite can be reversibly blocked. Visual performance of wild typeDrosophila in the optomotor response reflects receptor properties (visual acuity, spectral sensitivity and polarization sensitivity) expected for the R1–6 receptor subsystem. The notion of a ‘high sensitivity’ and a ‘high acuity’ state which was proposed earlier on the basis of experiments on various visual mutants is in agreement with the present results but their interpretation as reflecting properties of different receptor subsystems must be abandoned. Experimental data on wild type also suggest the existence of such an adaptational mechanism; this, however, remains to be demonstrated more conclusively.
    Materialart: Digitale Medien
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  • 9
    Digitale Medien
    Digitale Medien
    [s.l.] : Nature Publishing Group
    Nature 323 (1986), S. 154-156 
    ISSN: 1476-4687
    Quelle: Nature Archives 1869 - 2009
    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
    Notizen: [Auszug] Visual orientation of dipteran flies has been investigated for 40 years, mainly with emphasis on stimulus-induced turning responses5'6. Investigations rely on so-called open-loop experiments, in which the animal is partially immobilized so as to prevent its behaviour from interfering with the ...
    Materialart: Digitale Medien
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  • 10
    Digitale Medien
    Digitale Medien
    [s.l.] : Nature Publishing Group
    Nature 333 (1988), S. 19-20 
    ISSN: 1476-4687
    Quelle: Nature Archives 1869 - 2009
    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
    Notizen: [Auszug] As evidence accumulates that animals may require most of their genes for their brains and hence their behaviour, behavioural genes are becoming a focus of attention. One of the most advanced case studies is that of J.C. Hall, M. Rosbash and collaborators at Brandeis University. It concerns a gene ...
    Materialart: Digitale Medien
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