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  • 1
    ISSN: 0931-1890
    Schlagwort(e): Fagus Lactarius sp. Granules Acid phosphatase activity Seasonal changes
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract. This study concerned two perennial ectomycorrhizae of beech: Fagus sylvatica L./Lactarius blennius var. viridis and Fagus sylvatica L./Lactarius subdulcis, collected in a natural forest site. Nutritional exchanges existing between beech roots and fungi were evaluated over a seasonal cycle by studying the variations of ultrastructure, polyphosphate and nitrogenous granules and glycogen content (periodic acid thiocarbohydrazide silver test). The acid phosphatase activity, which is an indicator of the intensity of the physiological activity of symbiosis partners, was also revealed by transmission electron microscopy. The occurrence of the two ectomycorrhizae in the soil was not related to the climatic conditions. In winter, the number of polyphosphate and nitrogenous granules was high in the fungi, and glycogen was abundant in the cytoplasm of hyphae. In late winter, an intense acid phosphatase activity was revealed on plasmalemmas of live hyphae. It could indicate the beginning of fungal reserve mobilization, which progressively disappeared as spring progressed. In summer, glycogen was quasi non-existent and only few polyphosphate and nitrogenous granules were observed. At this period, the acid phosphatase activity was weak. Finally, in autumn and early winter, glycogen and granules were progressively restored in fungal structures. Relationships between changes of granules and glycogen content and the physiological states of each symbiont throughout the year are discussed.
    Materialart: Digitale Medien
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  • 2
    ISSN: 1365-3083
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Medizin
    Notizen: This paper shows that the seven HA306–320 specific T-cell clones isolated from one individual recognize the peptide complexed to both autologous HLA–DRB1*1101 and allogeneic HLA-DRB1*1601 (or DRB5*0201) molecules. For each T-cell clone, a single T-cell receptor (TCR) is involved in the recognition of these two different peptide-DR complexes as evidenced by cold target competition experiments. Yet, the seven T-cell clones express several different TCRs as judged by Vβ-Jβ usage and fine specificities. Furthermore, one representative clone has the same fine specificity for HA306–320 analogues mutated at epitopic residues irrespective of the use of DR1101 or DR1601 APC. These results suggest that structural differences between DRB1*1101 and DRB1*1601 (or DRB5*0201) do not dramatically influence the orientation of HA306–320 in the grooves such that most residues interacting with TCRs are conserved. In another individual, the same pattern of restriction, i.e. DR1101 + DR1601, was found for several HA306–320 specific clones. Two additional patterns, DR1101 + DR0801 and DR1101 + DR0801 + DR1601, were identified. By comparing DR sequences the authors found that DRB1*1101 and DRB1*1601 share four important motifs, i.e. β85–86, β67–71, β57 and β28–31 supposed to line three distinct HLA-DR pockets. Three of these motifs are also shared with DRB1*0801. All the results further support that the motif similarities allow the peptide to adopt very similar orientations in the cross-reacting DR molecules.
    Materialart: Digitale Medien
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  • 3
    Digitale Medien
    Digitale Medien
    Oxford, UK : Blackwell Publishing Ltd
    Allergy 51 (1996), S. 0 
    ISSN: 1398-9995
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Medizin
    Notizen: Drug-induced erythema multiforme is reported with many antimicrobial and antipyretic medications. We present one patient who experienced erythema multiforme and urticaria after administration of pyrazinamide for treatment of cutaneous tuberculosis. The side-effects of pyrazinamide include hepatic or dermatologic disorders, but erythema multiforme has not yet, to our knowledge, been described. Clinical features and discontinuation and reintroduction of the drug led us to consider pyrazinamide the cause of the erythema multiforme, but allergologic studies remained negative, except for the presence of circulating immune complexes. The mechanism of this eruption remains obscure. and definite withdrawal of pyrazinamide seems to be the best therapeutic choice.
    Materialart: Digitale Medien
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  • 4
    Digitale Medien
    Digitale Medien
    Oxford, UK : Blackwell Publishing Ltd
    Allergy 49 (1994), S. 0 
    ISSN: 1398-9995
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Medizin
    Notizen: We report two cases of soybean-lecithin-induced asthma in bakers. The patients experienced clinical symptoms in relation to an occupational exposure to this additive. Skin tests were positive with soybean lecithin, RAST showed a sensitization to soybean, and bronchial challenge tests were positive for a dilution of 10-3 with this allergen. The same tests remained negative among healthy and asthmatic controls. Soybean lecithin, a common additive in bakery, must be added to the list of numerous aeroallergens involved in baker's asthma.
    Materialart: Digitale Medien
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  • 5
    ISSN: 1434-601X
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Physik
    Notizen: Abstract. We report the results of an experiment, realized with the EUROBALL IV spectrometer, in which superdeformed (SD) states have been populated in 197,198Pb nuclei. Crosstalk transitions between two signature partner SD bands in 197Pb have been observed with an established M1 character, giving access to the magnetic properties of the $\nu$ [752]5/2 intruder orbital. Six new SD bands have been identified in 197,198Pb. The results are interpreted in the framework of cranked Hartree-Fock calculations. A mixing between quasi-particle excitations and an octupole vibration is suggested in the two SD isotopes.
    Materialart: Digitale Medien
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  • 6
    ISSN: 1434-601X
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Physik
    Notizen: Abstract. The 110,112 Rh and 114,116 Ag nuclei have been produced as fission fragments in the fusion reaction 18 O+208 Pb at 85 MeV. Their level schemes have been built from gamma-rays detected using the Euroball IV array. High-spin states of these neutron-rich nuclei have been identified for the first time. The yrast structures consist of rotational bands in which the odd proton occupies the $\pi g_{9/2}$ sub-shell and the odd neutron the $\nu h_{11/2}$ sub-shell. The evolution of the $\pi g_{9/2} \otimes \nu h_{11/2}$ band structure is analyzed as a function of the neutron number.
    Materialart: Digitale Medien
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  • 7
    Digitale Medien
    Digitale Medien
    Springer
    Theoretical and applied genetics 98 (1999), S. 107-112 
    ISSN: 1432-2242
    Schlagwort(e): Key words DNA fingerprinting ; ISSR-PCR ; Solanum tuberosum ; Resolving Power
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  Commercial scale fingerprinting of potato cultivars is made difficult by the need for speed, reliability and the ability to distinguish between large numbers of genotypes. There are also problems in extrapolating the results of small experimental studies to predict the performance of techniques or primers for larger applications. The potential of ISSR-PCR for fingerprinting purposes was evaluated using four primers on 34 potato cultivars. The complex band profiles generated were reproducible between repeat PCRs, DNA extractions, electrophoreses and gel scorings. Two primers were each able to distinguish all cultivars. The combined use of any two of the four primers also allowed complete diagnosis. It is concluded that ISSR-PCR provides a quick, reliable and highly informative system for DNA fingerprinting that is amenable for routine applications. Two possible correlates of the ability of primers to distinguish between genotypes were then examined. Marker Index failed to correlate significantly with genotype diagnosis, but a strong and seemingly linear relationship was observed between Resolving Power of a primer and its ability to distinguish genotypes (r2=0.98). Resolving Power of one or a pair of primers was found to provide a moderately accurate estimate of the number of genotypes identified. Possible implications for future studies on DNA fingerprinting are discussed.
    Materialart: Digitale Medien
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  • 8
    ISSN: 1434-601X
    Schlagwort(e): PACS. 21.10.Re Collective levels – 23.20.En Angular distribution and correlation measurements – 23.20.Lv Gamma transitions and level energies – 27.80.+w 190 ≤ A ≤ 219
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Physik
    Notizen: Abstract: This work has established eight cross-talk transitions between the two signature partner superdeformed (SD) bands in 197Pb with the EUROBALL IV spectrometer. Directional correlations from oriented states measurements confirm the ΔI = 1 character of these transitions. The flat behaviour of the dynamical moment of inertia and the agreement between the experimental and microscopic HF+BCS values of (g K - g R)K/Q 0 suggest that the configuration of the SD bands is based upon the υ[752]5/2- neutron intruder orbital. The derived effective spin gyromagnetic factor g s eff is found to be not quenched, and is close to the theoretical g s free value.
    Materialart: Digitale Medien
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  • 9
    ISSN: 1434-601X
    Schlagwort(e): PACS. 21.10.Re Collective levels – 21.60.Ev Collective models – 23.20.Lv Gamma transitions and level energies – 27.80.+w 190 ?A? 219
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Physik
    Notizen: Abstract: We report the identification of six new superdeformed (SD) bands in 197,198Pb observed with the EUROBALL IV spectrometer. The results are interpreted in the framework of cranked Hartree-Fock calculations with approximate projection on the particle number by means of the Lipkin-Nogami method. A mixing between quasi-particle excitations and an octupole vibration is suggested in the two SD isotopes. We have estimated the ordering of the neutron valence orbitals and confirm indirectly a N = 118 SD gap.
    Materialart: Digitale Medien
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  • 10
    Digitale Medien
    Digitale Medien
    Springer
    Journal of low temperature physics 113 (1998), S. 787-798 
    ISSN: 1573-7357
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Physik
    Notizen: Abstract We have studied the spreading of helium-4 on cesium substrates with random disorder. When the characteristic length scale of the disorder is of the order of 20 micrometers, we have been able to measure the roughness exponents of the contact line, and found that they are in agreement with theoretical predictions. The temperature dependence of the roughness is also consistent with the theory. However, the same model predicts an hysteresis much smaller than the measured one. Better cesium substrates with optical quality yield a very straight contact line but the same large hysteresis. This hysteresis is presumably due to the pinning of the line on microscopic defects of the evaporated cesium film. The velocity of the contact line increases exponentially with the applied force, suggesting that the depinning is thermally activated.
    Materialart: Digitale Medien
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