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  • 1
    Digitale Medien
    Digitale Medien
    Cupulogenesis and glycoconjugates in the labyrinthine ampulla as revealed by WGA-gold labeling (1987)
    Springer
    European archives of oto-rhino-laryngology and head & neck 244 (1987), S. 112-116 
    Details
    ISSN: 1434-4726
    Schlagwort(e): Wheat germ agglutinin-gold ; Ampulla ; Mongolian gerbil ; Cupulogenesis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary We studied the distribution of wheat germ agglutinin (WGA)-bindable glycoconjugates in the vestibular ampulla of mongolian gerbils. WGA was conjugated with gold particles and applied to Lowicryl K4M sections of the ampulla. WGA-binding sites were found on the cupula and some of the secretory granules and Golgi apparatuses in the supporting cells of the sensory epithelia. The granules were seen to secrete into the endolymphatic space through reticular membrane. It is likely, therefore, that glycoconjugates are glycosylated at the Golgi apparatus in the supporting cells, stored in the granules, and secreted through the reticular membrane into the endolymphatic space to be used as a component of the cupula. The cell membranes of various cells, connective tissue filaments in the perilymphatic space and the cytoplasm of melanocytes were also labeled with WGA-gold.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00458560
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  • 2
    Digitale Medien
    Digitale Medien
    Determination of lipid A in human middle ear effusions with the competitive ELISA technique (1989)
    Springer
    European archives of oto-rhino-laryngology and head & neck 246 (1989), S. 79-82 
    Details
    ISSN: 1434-4726
    Schlagwort(e): Lipid A ; Otitis media with effusion ; ELISA
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary Lipid A is a covalently bound component of gram-negative bacterial lipopolysaccharides and is the biologically active component in endotoxin. In order to detect and quantitate lipid A in middle ear effusions (MEE) from patients with otitis media with effusion (OME), antisera against lipid A were produced in mice by repeated immunization with conjugates using lipid A from Salmonella minnesota Re595 and bovine serum albumin. As a result, a competitive enzyme-linked immunosorbent assay (ELISA) was developed that was specific for lipid A. From the ELISA results, 10 out of 15 samples from the serous type MEE (mean, 13.57±2.17 μg/ml) and 5 out of 6 from the mucoid type MEE (17.73±4.34 μg/ml) were positive. These findings indicate that lipid A may contribute to the pathogenesis of both types of human OME.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00457458
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  • 3
    Digitale Medien
    Digitale Medien
    Glycoconjugate histochemistry in the glycocalyx of guinea pig middle ear mucosa (1990)
    Springer
    European archives of oto-rhino-laryngology and head & neck 247 (1990), S. 291-295 
    Details
    ISSN: 1434-4726
    Schlagwort(e): Middle ear ; Lectin ; Glycocalyx
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary An attempt was made to determine the composition of oligosaccharide side chains in the apical cell surface glycocalices of the middle ear epithelium. This glycoconjugate is located on the innermost luminal surface, but its function is still unknown. The lectins evaluated included Triticum vulgare, Canavalia ensiformis, Maclura pomifera, Arachis hypogaea, Ricinus communis I (RCA-I), and Limax flavus. The staining results obtained with the six different lectin probes in the Lowicryl K4M-embedded guinea pig middle ear mucosa suggest that the glycocalyx contains β-d-N-acetylglucosamine, sialic acid, and β-d-galactose in the major carbohydrate moiety. The present study also indicates that a polarity exists on the cell surfaces in the distribution pattern of the carbohydrate component, especially those of β-galactosyl residues, as revealed by RCA-I binding. This may imply functional separation of the epithelial cells.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00176540
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  • 4
    Digitale Medien
    Digitale Medien
    Cell-surface glycoconjugate layer in the tubotympanic mucosa of the guinea pig as revealed by wheat germ agglutinin/gold labelling (1995)
    Springer
    European archives of oto-rhino-laryngology and head & neck 252 (1995), S. 249-254 
    Details
    ISSN: 1434-4726
    Schlagwort(e): Middle ear ; Glycoconjugate ; Wheat germ agglutinin ; Electron microscopy ; Guinea pig
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract To evaluate the protective function of the mucous blanket (MB) against lectin substances, we examined at the ultrastructural level whether intraluminal colloidal gold-labelled wheat germ agglutinin (WGA) could enter the MB-covered epithelial cell surface of the guinea pig tubotympanic mucosa. Post-embedding staining with WGA/gold on thin tissue sections was done in parallel for comparison. The cell surface glycoconjugate of the eustachian tubal and transitional epithelium had a typical bilayered structure: the outer MB and the microvilli-associated glycocalyx (MAG), which were interposed by the interciliary fluid zone. In squamous epithelium of the distal middle ear, the MB adhered to the MAG, thereby forming a monolayered coat of glycoconjugates at the cell surface. In the pre-embedding staining, WGA/gold did not bind with the MB and MAG in the eustachian tube, and exclusively bound with MB in the transitional area. Direct binding was also found with MAG and the apical plasmic membrane in the squamous epithelium. These findings indicate that MAG is occluded by MB lined with the interciliary fluid zone for luminal access of lectin at the proximal lumina of the tubotympanic epithelium. It is also suggested that MB existing at two sites possesses a different WGA-binding capacity: shielding as a “dust cover” in the eustachian tube and entrapping as a “flypaper” against lectin in the transitional area of the middle ear.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00179920
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  • 5
    Digitale Medien
    Digitale Medien
    Teratoma of the soft palate with multifocal cyst formation in the tongue and the brain (1986)
    Springer
    European archives of oto-rhino-laryngology and head & neck 243 (1986), S. 320-323 
    Details
    ISSN: 1434-4726
    Schlagwort(e): Teratoma ; Soft palate ; Arachnoid cyst ; Hydrocephalus
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary We report a case of a solid and cystic teratoma appearing in the soft palate that was associated with a cyst in the tongue and a huge cystic formation in the arachnoid of the brain. Histologically, the tumor of the soft palate contained elements of all three germ layers, with a predominance of mature glial tissues. This typical teratoma and the two other concurrent lesions were all located on the same side of the head, and were thought to have a common embryonic origin. These multifocal tumors may represent an incomplete manifestation of a “teratoma complex”.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00460210
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