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  • 1
    Digitale Medien
    Digitale Medien
    Accumulation of aluminium in the cell wall pectin in cultured tobacco (Nicotiana tabacum L.) cells treated with a combination of aluminium and iron (1999)
    Oxford, UK : Blackwell Science Ltd.
    Plant, cell & environment 22 (1999), S. 0 
    Details
    ISSN: 1365-3040
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie
    Notizen: In nutrient medium, aluminium (Al) accumulation in tobacco cells occurs only in the presence of ferrous ion [Fe(II)]. The localization of Al was examined to elucidate a mechanism of Al accumulation. After the digestion of Al-treated cells with cellulase and pectolyase together, the resulting spheroplasts contained as much Al as the intact cells. However, the cell walls isolated from Al-treated cells also contained as much Al as the intact cells. Comparison of sugar and Al contents in polysaccharide components extracted chemically from cell walls isolated from intact cells and spheroplasts revealed that the enzymes digested most of the cellulose and hemicellulose, but only half of the pectin, and that Al mainly existed in the pectin remaining in the spheroplasts. Gel-permeation chromatography of the pectin fraction (NH4-oxalate extract) from the cell walls of the intact cells indicated that Al was associated with small polysaccharides of approximately 3–7 kDa. These results suggest that a minor part of pectin is a major site of Al accumulation. The content of cell wall pectin increased during Al treatment in nutrient medium. Taken together, we hypothesize that Al may bind to the pectin newly produced during Al treatment.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1046/j.1365-3040.1999.00467.x
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  • 2
    Digitale Medien
    Digitale Medien
    Late development of antidesmoglein 1 antibodies in pemphigus vulgaris: correlation with disease progression (1999)
    Oxford, UK : Blackwell Science Ltd
    British journal of dermatology 141 (1999), S. 0 
    Details
    ISSN: 1365-2133
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Medizin
    Notizen: The coexistence of antidesmoglein 3 (Dsg3) and antidesmoglein 1 (Dsg1) autoantibodies is well described in patients with pemphigus vulgaris (PV); however, there is little evidence of sequential development of these two autoantibodies. Autoantibody responses to Dsg3 and Dsg1 were studied in seven PV patients over time by enzyme-linked immunosorbent assay, using baculovirus expressed recombinant fusion proteins. All patients had anti-Dsg3 IgG antibodies at presentation. Two patients developed anti-Dsg1 later in the course of the disease. The transition in autoantibody profile was associated with disease progression to generalized PV involving mucous membranes and skin in both patients; one patient initially presented with a predominantly mucosal phenotype, the other with herpetiform pemphigus-like features. These findings demonstrate that there is an extension of autoimmune response from anti-Dsg3 only to both anti-Dsg3 and anti-Dsg1 in some patients, which is associated with an alteration in clinical expression in PV.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1046/j.1365-2133.1999.03209.x
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  • 3
    Digitale Medien
    Digitale Medien
    Ultrastructure of the Thyroid Gland of the One-humped Camel (Camelus dromedarius) (1999)
    Oxford, UK : Blackwell Science, Ltd
    Anatomia, histologia, embryologia 28 (1999), S. 0 
    Details
    ISSN: 1439-0264
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Medizin
    Notizen: Follicular epithelium of the thyroid gland of the one-humped camel was examined by light and electron microscopy. It consisted of a single type of epithelial cell which varied from flattened to columnar in shape. Follicular epithelial cells were characterized by the presence of markedly dilated cisternae of rough-surfaced endoplasmic reticulum, well developed Golgi apparatus, abundant small vesicles (150 nm to 200 nm in diameter) in the apical cytoplasm, and electron-dense colloid droplets measuring from 250 nm to 1600 nm. Follicular epithelial cells frequently showed apocrine secretion into colloidal lumens. Apocrine protrusions with smooth surface were dome-like or balloon-like structures and contained a fine granular matrix. These findings indicate that the morphological features of the follicular epithelial cells of the thyroid gland of the camel are essentially similar to those of mammals except for the presence of apocrine secretion, which is unique to the camel.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1046/j.1439-0264.1999.00152.x
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  • 4
    Digitale Medien
    Digitale Medien
    Etidronate (EHDP) Inhibits Osteoclastic-Bone Resorption, Promotes Apoptosis and Disrupts Actin Rings in Isolate-Mature Osteoclasts (1999)
    Springer
    Calcified tissue international 64 (1999), S. 219-223 
    Details
    ISSN: 1432-0827
    Schlagwort(e): Key words: EHDP — Osteoclast — Apoptosis — Actin rings.
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin , Physik
    Notizen: Abstract. Bisphosphonates, therapeutic reagents against tumoral bone diseases (Paget's disease or osteoporosis), are potent inhibitors of bone resorption. The mechanisms by which they directly act on mature osteoclasts remain unclear. Using a recently developed technique for isolation of highly purified mammalian mature osteoclasts, we demonstrated that etidronate [ethane-1-hydroxy-1,1-diphosphonate (EHDP), 1-hydroxy-1,1-ethylidenebisphosphonate], inhibited directly osteoclastic bone-resorbing activity by pit assay. In addition, EHDP also directly induced apoptosis and disrupted actin rings in osteoclasts. The data support previous data on non-purified osteoclasts and results in vivo.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s002239900606
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  • 5
    Digitale Medien
    Digitale Medien
    A single-photon turnstile device (1999)
    [s.l.] : Macmillan Magazines Ltd.
    Nature 397 (1999), S. 500-503 
    Details
    ISSN: 1476-4687
    Quelle: Nature Archives 1869 - 2009
    Thema: Biologie , Chemie und Pharmazie , Medizin , Allgemeine Naturwissenschaft , Physik
    Notizen: [Auszug] Quantum-mechanical interference between indistinguishable quantum particles profoundly affects their arrival time and counting statistics. Photons from a thermal source tend to arrive together (bunching) and their counting distribution is broader than the classical Poisson limit. Electrons from ...
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1038/17295
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  • 6
    Digitale Medien
    Digitale Medien
    NEXAFS studies on the structure of perfluoroalkyl carbonic acid Langmuir Blodgett films (1999)
    Chester : International Union of Crystallography (IUCr)
    Journal of synchrotron radiation 6 (1999), S. 796-798 
    Details
    ISSN: 1600-5775
    Quelle: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Thema: Geologie und Paläontologie , Physik
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1107/S0909049598017348
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  • 7
    Digitale Medien
    Digitale Medien
    Effective escape depth of photoelectrons for hydrocarbon films in total electron yield measurement as C K-edge (1999)
    Chester : International Union of Crystallography (IUCr)
    Journal of synchrotron radiation 6 (1999), S. 803-804 
    Details
    ISSN: 1600-5775
    Quelle: Crystallography Journals Online : IUCR Backfile Archive 1948-2001
    Thema: Geologie und Paläontologie , Physik
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1107/S0909049599004033
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  • 8
    Digitale Medien
    Digitale Medien
    Crystal lattice quantum computer (1999)
    Springer
    Applied physics 68 (1999), S. 1-8 
    Details
    ISSN: 1432-0630
    Schlagwort(e): PACS: 03.67.Lx; 76.60.k; 75.50.Ee
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Maschinenbau , Physik
    Notizen: 31 P nucleus can be used to represent a quantum bit (‘qubit’) with a relatively long relaxation time. In a CeP crystal lattice, 31P nuclei are periodically situated in three dimensions at distances of about 6 Å. The application of a static magnetic field gradient in one direction causes differences in the Zeemanfrequencies of separate nuclei. This allows thousands of distinct qubits to be individually addressed. Initializations of the qubits can be done efficiently by the Pound–Overhauser double resonance effect on the nuclear spins and the antiferromagnetically ordered 4f electron spins of cerium ions. Logic operations can be performed by simple pulse sequences, and computational results after logic operations can be measured by the nuclear magnetic resonance of neighboring nuclei, or the electron resonance of neighboring 4f electrons of cerium ions.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s003390050846
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  • 9
    Digitale Medien
    Digitale Medien
    Fibrin Plugging as a Cause of Microcirculatory Occlusion During Photodynamic Therapy (1999)
    Springer
    Lasers in medical science 14 (1999), S. 129-135 
    Details
    ISSN: 1435-604X
    Schlagwort(e): Keywords: Microcirculation; Mono-l-aspartyl chlorin-e-6 (Npe-6); Photodynamic therapy (PDT); Vascular shut-down
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin , Physik , Technik allgemein
    Notizen: Abstract. An experimental system that allows the white light observation of rapid changes in vessels without disturbance by red laser light was used. Mice were injected with mono-l-aspartyl chlorin-e-6 (Npe-6) i.v. via the tail vein and were immediately exposed to laser light. White emboli were observed forming on the inside of the vessel walls within seconds after commencement of light exposure. Emboli adhered to vessel walls and caused vascular obstruction. Light microscopy of the exposed material using fibrin staining was performed. Electron microscopy on the same material was also carried out. The embolisation time was influenced by both drug dose and laser power. With low laser power, it took a long time to stop the blood flow. Fibrin staining revealed the white emboli to be composed of fibrin. Electron microscopy findings revealed damage to endothelial cells and platelet aggregation. This study suggests that two main mechanisms (direct cellular damage and vascular shut-down ) might actually be complementary and synergistic in the production of vascular lesions using photodynamic therapy.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s101030050034
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  • 10
    Digitale Medien
    Digitale Medien
    Deletion of a large genomic segment in tobacco varieties that are resistant to potato virus Y (PVY) (1999)
    Springer
    Molecular genetics and genomics 262 (1999), S. 822-829 
    Details
    ISSN: 1617-4623
    Schlagwort(e): Key words Recessive resistance ; Potato virus Y ; Randomly amplified polymorphic DNA (RAPD) ; Nicotiana tabacum L.
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Recessive alleles (va, va 1 , va 2 , etc) of the tobacco Va locus confer resistance to potato virus Y (PVY). To elucidate the mechanism underlying this resistance, we attempted to identify randomly amplified polymorphic (RAPD) markers that reveal polymorphism between two nearly isogenic lines (NILs) that differ in their susceptibility to PVY. Using each of 500 primers and 800 pairs of primers, we identified over 100 RAPD fragments that differed between the NILs. We applied these RAPD primers or primer combinations to an F2 population obtained from a cross between the susceptible line BY4 and the resistant va 2 -bearing NIL, F55. It was found that only 10 RAPD markers were polymorphic between resistant and susceptible plants. Unexpectedly, these markers were all linked to Va. All 10 RAPD markers were present in all 8 susceptible varieties tested. At least one RAPD marker was not detected in 8 out of 10 resistant varieties. Southern analysis revealed that the sequences of markers were not present in the genomes of resistant varieties, and the markers were found in individually distinct positions on the chromosomes of susceptible tobacco varieties. These results strongly suggest that the resistance conferred by va is due to deletions at the Va locus governing susceptibility to PVY.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s004380051146
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