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  • 1
    Digitale Medien
    Digitale Medien
    Springer
    Theoretical and applied genetics 89 (1994), S. 206-210 
    ISSN: 1432-2242
    Schlagwort(e): Lens culinaris ; Mitochondrial DNA ; Maternal inheritance ; Biparental mitochondrial inheritance ; Restriction fragment length polymorphism
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Restriction fragment analysis was used to examine the inheritance of lentil mitochondrial DNA (mtDNA) in F1 and F5 progeny from intrasubspecific (Lens culinaris ssp. culinaris) crosses and in F1 progeny from intersubspecific (Lens culinaris ssp. orientalis x L. culinaris ssp. culinaris) crosses. Southern blots of digested parental and progeny DNA were hybridized to heterologous maize mtDNA probes specific to coxI and atp6 genes. Two restriction fragment polymorphisms separated L.c. ssp. culinaris ‘Laird’ and ‘Eston’ from L.c. ssp. culinaris ‘ILL5588’, and one restriction fragment polymorphism distinguished L.c. ssp. culinaris ‘Laird’ and ‘Eston’ from L.c. ssp. orientalis ‘LO4’. Twelve of 13 f1 progeny and all F5 progeny from the intrasubspecific crosses, and all F1 progeny from intersubspecific crosses had only maternal mtDNA restriction fragments. One f1 plant from an ‘Eston’ x ‘ILL5588’ cross inherited mtDNA fragments from both parents. Nuclear DNA inheritance was biparental in all F1 progeny.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Springer
    Theoretical and applied genetics 90 (1995), S. 607-610 
    ISSN: 1432-2242
    Schlagwort(e): Lens culinaris ; Chloroplast DNA Maternal plastid inheritance ; Biparental plastid inheritance ; Restriction fragment length polymorphism
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Restriction fragment analysis was used to study the inheritance of chloroplast DNA (cpDNA) in F1 progeny from crosses between Lens culinaris ssp. orientalis and L. culinaris ssp. culinaris. Twenty-five combinations of 11 restriction enzymes and three heterologous probes from Petunia hybrida cpDNA were used to screen six accessions of L.c. culinaris and one accession of L. c. orientalis for restriction fragment length polymorphisms (RFLPs). No variation in cpDNA was observed within the subspecies L. c. culinaris, but the L. c. orientalis accession was unambiguously distinguished from all six L. c. culinaris accessions by two RFLPs. Of ten F1 progeny from L. c. orientalis x L. c. culinaris crosses, nine had only maternal cpDNA restriction fragments but one F1 plant inherited cpDNA fragments from both parents. Nuclear DNA inheritance was biparental in all ten F1 progeny.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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