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  • 11
    Electronic Resource
    Electronic Resource
    Hybrid genes in the analysis of transformation conditions (1987)
    Springer
    Plant molecular biology 8 (1987), S. 363-373 
    Details
    ISSN: 1573-5028
    Keywords: transformation ; MgCl2-PEG-electroporation ; competence ; protoplasts
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Direct gene transfer into plant protoplasts has been recently developed, and conditions for high frequency transformation of SR1 tobacco protoplasts established. In this paper we analyse numerous transformation parameters in a comparative study on SR1 Nicotiana tabacum and N. plumbaginifolia, and report on a simple chemical technique for very efficient protoplast transformation. It is based on the synergistic interaction of MgCl2 and PEG. The technique yielded up to 1400 transformants per 3×105 treated N. tabacum protoplasts (up to 4.8% of the survivors, late selected clones). Using N. plumbaginifolia, the frequencies were 10-fold lower, indicating that the ‘competence’ for transformation has a species-specific component.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00015814
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    Paper (German National Licenses)
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  • 12
    Electronic Resource
    Electronic Resource
    The histogenesis of somaclones from tomato (Lycopersicon esculentum Mill.) cotyledons (1988)
    Springer
    Protoplasma 142 (1988), S. 156-163 
    Details
    ISSN: 1615-6102
    Keywords: Tomato ; Lycopersicon esculentum ; Somaclonal variation ; Plant regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A histological study ofin vitro cultured cotyledonary expiants of tomato (Lycopersicon esculentum) was performed in order to determine the site (differentiated tissue or developing callus) and the mode of plant regeneration. Results have shown that callus develops at the excision sites of cotyledonary expiants and that shoots are formed exclusively within the unorganized callus: excision areas are the only morphogenetic sites and the proximal excision is the preferred site for plant regeneration. Shoots differentiate by organogenesis within the superficial region of the callus. Few neocambial cells cooperate in the neoformation. Origin from a single cell is highly unlikely since rarely observed single activated cells never developed into shoots. Regenerated plants may be chimeras if invitro culture induces genetic diversity in the initial cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01290872
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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