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  • 1
    Electronic Resource
    Electronic Resource
    Porokeratosis: immunosuppression and exposure to sunlight (1987)
    Oxford, UK : Blackwell Publishing Ltd
    British journal of dermatology 116 (1987), S. 0 
    Details
    ISSN: 1365-2133
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: Four cases of disseminated superficial aclinic porokeratosis are described, all in patients who had been given immunosuppressive treatment, which might have been a contributing factor in the development of the condition. The possible relationship of immunosuppressive actinic porokeratosis to viral infection is also discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2133.1987.tb05798.x
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  • 2
    Electronic Resource
    Electronic Resource
    Somaclonal variation versus chemically induced mutagenesis in tomato (Lycopersicon esculentum L.) (1987)
    Springer
    Theoretical and applied genetics 74 (1987), S. 733-738 
    Details
    ISSN: 1432-2242
    Keywords: Tomato ; Somaclonal variation ; Chemical mutagenesis ; Mutants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A comparison was made of the type and frequency of mutational events found in the progeny of tomato plants regenerated after one passage in vitro with those induced by chemical mutagenesis with ethyl methane sulphonate. Several mutants were recovered in the progeny of regenerated and mutagenized plants of two cultivars of tomato. They can be grouped into the following categories: seedling lethality, male sterility, resistance to Verticillium, short stature, change in number of lateral shoots or in leaf shape. The results indicate that the two sources of variability differ in their effect, changing the spectrum and frequency of the mutants as well as, at least in some cases, their pattern of segregation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00247550
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Nuclear DNA amplification in cultured cells of Oryza sativa L. (1987)
    Springer
    Theoretical and applied genetics 74 (1987), S. 65-70 
    Details
    ISSN: 1432-2242
    Keywords: DNA amplification ; Cultured cells ; Dot hybridization ; Oryza sativa L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Highly repeated nuclear DNA sequences from suspension cultured cells of Oryza sativa L. cv. ‘Roncarolo’ have been cloned in pBR322. Ten clones with specific digestion patterns have been randomly selected. Nine sequences appear to be organized in a clustered tandem array while one is interpersed in the rice genome. The clones have been used to gather information on: (a) their modulation in cultured cells as compared to whole plant and (b) their distribution in different rice cultivars belonging to the Japonica or Indica subspecies of Oryza sativa L. Hybridization with nuclear DNA isolated either from suspension or from seedlings of the ‘Roncarolo’ cultivar revealed extensive quantitative variations, with most cloned sequences showing amplification (up to 75-fold) in cultured cells. Hybridization with nuclear DNA isolated from seedlings or suspension cultured cells from different cultivars belonging to the Japonica or to the Indica sub-species of O. sativa have shown that (a) amplification also occurs in a similar pattern in the case of DNA from the other tested suspension cultured cell types but not in the case of DNA from seedlings; (b) in some cases the tested sequences show minor but significant variations in different rice accessions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00290085
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Hybrid genes in the analysis of transformation conditions (1987)
    Springer
    Plant molecular biology 8 (1987), S. 363-373 
    Details
    ISSN: 1573-5028
    Keywords: transformation ; MgCl2-PEG-electroporation ; competence ; protoplasts
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Direct gene transfer into plant protoplasts has been recently developed, and conditions for high frequency transformation of SR1 tobacco protoplasts established. In this paper we analyse numerous transformation parameters in a comparative study on SR1 Nicotiana tabacum and N. plumbaginifolia, and report on a simple chemical technique for very efficient protoplast transformation. It is based on the synergistic interaction of MgCl2 and PEG. The technique yielded up to 1400 transformants per 3×105 treated N. tabacum protoplasts (up to 4.8% of the survivors, late selected clones). Using N. plumbaginifolia, the frequencies were 10-fold lower, indicating that the ‘competence’ for transformation has a species-specific component.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00015814
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    Paper (German National Licenses)
    Fulltext
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