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  • 11
    Electronic Resource
    Electronic Resource
    Effect of alignment of the transplanted graft extracellular matrix on cellular repopulation and newly synthesized collagen (1998)
    Springer
    Archives of orthopaedic and trauma surgery 117 (1998), S. 215-221 
    Details
    ISSN: 1434-3916
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract This study examines the effect of alignment of the transplanted graft extracellular matrix on cellular repopulation and new collagen synthesis. The lateral half of the patellar tendon was harvested as a tendon graft from Lewis rats and frozen at –80° C. In order to maintain the original alignment of the graft extracellular matrix, the graft was transplanted to a same size defect in the patellar tendon of other Lewis rats (group I). For controls, the graft was transplanted in a lax condition after excision of only the distal half of the lateral side of a patellar tendon (group II). After transplantation, six animals in each group were killed at 3, 7, 14, and 28 days. Cellular repopulation was assessed by using fibrillar-actin (F-actin) labeling with rhodamine-phalloidin, and new collagen synthesis was detected by means of a polyclonal antibody against type III collagen aminopropeptide (pN collagen III). Collagen fibril profiles were observed under the transmission electron microscope. On the 3rd day after transplantation, no specific fluorescence was detected in either group. Specific labeling for F-actin and pN collagen III, however, was observed at both ends of the graft in both groups at 1 week and throughout the graft at 2 weeks after transplantation. Consistent with the actin bundles’ orientation, pN collagen III was aligned parallel to the longitudinal axis of the graft in group I. Collagen fibrils with a smaller diameter, mixed evenly and everywhere with a larger diameter, increased gradually in group I. However, smaller collagen fibrils in group II increased more slowly and were distributed unevenly. In summary, the dense collagen arrangement in the native patellar tendon determined the alignment of the repopulating cells, and the distribution of newly synthesized collagen might be affected by the actin cytoskeleton within the repopulating cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004020050232
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  • 12
    Electronic Resource
    Electronic Resource
    Do plant and soil systems contribute to global carbon cycling as a sink of CO2? (1997)
    Springer
    Nutrient cycling in agroecosystems 49 (1997), S. 287-293 
    Details
    ISSN: 1573-0867
    Keywords: carbon cycling ; carbon dioxide sequestration ; carbon sink ; modeling ; terrestrialecosystem
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Major conclusions from our two projects focussing on carbon cycling in terrestrial ecosystems are as follows: 1) A rural system or a farmland system tends to be a source of carbon dioxide. However, it was possible to increase carbon dioxide sequestration in soil by changing soil or paddy/upland-field management systems. 2) A model simulation showed that a carbon budget in a natural forest was balanced before cutting but the large minus (source) was observed just after cutting. But the balance changed from minus to plus (sink) in 10 years after cutting. Nearly the same amounts of carbon as that stocked in the timbers before harvesting accumulated in 70-80 years after the cutting. 3) These results indicate the possibility of soils in terrestrial ecosystems as the major sink of atmospheric carbon dioxide.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009724723121
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  • 13
    Electronic Resource
    Electronic Resource
    CT differentiation between necrotic and nonnecrotic small bowel in closed loop and strangulating obstruction (1999)
    Springer
    Abdominal imaging 24 (1999), S. 120-124 
    Details
    ISSN: 1432-0509
    Keywords: Key words: Small bowel—Closed loop—Strangulating obstruction—Intestinal necrosis—Computed tomography—Multivariate analysis.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Background: The purpose of this study was to evaluate computed tomographic (CT) findings for predicting the presence of intestinal necrosis in patients with closed loop and strangulating obstruction of the small bowel. Methods: Twenty-five patients with surgically confirmed closed loop and strangulating obstruction were divided into two groups with (n= 16) and without (n= 9) intestinal necrosis. By using univariate and multivariate statistical procedures, we evaluated the differences in CT findings between the two groups on the basis of the following six findings: bowel dilatation of strangulated loops (bowel dilatation), wall thickening of strangulated intestines (wall thickening), ascites, vascular dilatation of affected mesenteries (vascular dilatation), elevation of mesenteric attenuation (mesenteric attenuation), and radial distribution of the mesenteric vessels (radial distribution). Results: Of the six findings, ascites, vascular dilatation, mesenteric attenuation, and radial distribution provided significant discriminating findings between the two groups on univariate analysis. On multivariate analysis, mesenteric attenuation was the most important discriminative factor, followed by radial distribution and ascites. Using these three parameters, the CT was correlated with the surgical findings in 15 of the 16 patients in the necrosis group (sensitivity = 93.8%) and in eight of the nine patients in the nonnecrosis group (specificity = 88.9%). The overall accuracy was 92.0%. Conclusions: Mesenteric attenuation, radial distribution, and ascites, depicted on CT differentiate well between necrosis and nonnecrosis of the small bowelin patients with closed loop and strangulating obstruction.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002619900458
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  • 14
    Electronic Resource
    Electronic Resource
    Molecular mapping of a translocation breakpoint at 14q13 in a patient with mirror-image polydactyly of hands and feet (1997)
    Springer
    Human genetics 〈Berlin〉 99 (1997), S. 450-453 
    Details
    ISSN: 1432-1203
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Mirror hands and feet (MIM, 135750) is a rare congenital anomaly, and mirror-image polydactyly is considered to be a variant of mirror hands and feet. To our knowledge, seven patients with the disorder have been reported in the literature. Parent-to-child transmission was reported in two families, which may indicate a single-gene defect inherited in an autosomal dominant fashion. We had previously encountered a boy with mirror-image polydactyly whose karyotype showed 46,XY,t(2;14) (p23.3;q13) de novo. We hypothesized that at least one of the putative genes responsible for the determination of an anterior-posterior limb pattern is disrupted by a translocation breakpoint. In this study, we identified a yeast artificial chromosome clone spanning a translocation breakpoint at 14q13, and the breakpoint was confirmed to be located between two loci, AFM200ZH4 and D14S306, within a genetic distance of 0.6 cM.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004390050387
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  • 15
    Electronic Resource
    Electronic Resource
    Amlodipine lowers blood pressure without increasing sympathetic activity or activating the renin-angiotensin system in patients with essential hypertension (1997)
    Springer
    European journal of clinical pharmacology 53 (1997), S. 197-201 
    Details
    ISSN: 1432-1041
    Keywords: Key words Amlodipine ; Hypertension
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Medicine
    Notes: Abstract Objective: Recent clinical studies suggest that the reflex increase in sympathetic nervous activity accompanying a reduction in blood pressure may contribute to the untoward effects of dihydropyridine calcium antagonists. The aim of this study was to examine whether plasma noradrenaline levels and renin activity are increased with the reduction of blood pressure during the initial phase of administration of the long-acting dihyropyridine calcium antagonist amlodipine. Methods: The effects of amlodipine on ambulatory blood pressure and on diurnal variations in plasma noradrenaline and renin activity were examined 1, 4, and 7 days after the start of amlodipine administration in eight inpatients with essential hypertension. Results: The 24-h mean systolic and diastolic blood pressure on day 7 was significantly lower than it was 1 day before the start of treatment. There was no change in the mean heart rate. The mean trough to peak ratios of systolic and diastolic blood pressure of seven patients were 61% and 71%, respectively. Diurnal patterns of plasma noradrenaline levels and renin activity 1, 4, and 7 days after the start of amlodipine administration were unchanged. Conclusion: The antihypertensive effects of amlodipine were of slow onset and long duration and were not accompanied by an increase in sympathetic activity or activation of the renin-angiotensin system.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002280050362
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  • 16
    Electronic Resource
    Electronic Resource
    Sol–gel synthesis of zircon–effect of addition of lithium ions (1998)
    Springer
    Journal of materials science 33 (1998), S. 4821-4828 
    Details
    ISSN: 1573-4803
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract The sol–gel method was applied to the preparation of zircon, ZrSiO4 powders. The addition of lithium ions to the ZrO2·SiO2 gels was found to be very effective to produce zircon at temperatures as low as 800–900°C. The zircon formation process in the gels was followed by using X-ray diffraction and i.r. analysis methods. A possible mineralizing mechanism of lithium ions, which is essentially different from the previous ones for the solid-state reaction, was proposed. Namely, the formation of orthosilicate such as Li4SiO4 as an intermediate compound was considered to trigger the zircon formation at low heat-treatment temperatures.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1004478229360
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  • 17
    Electronic Resource
    Electronic Resource
    Isolation of microdissection clones from rat Chromosome 10 (1995)
    Springer
    Mammalian genome 6 (1995), S. 216-218 
    Details
    ISSN: 1432-1777
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00293019
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