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  • 11
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    Free Will: A Reply to Mr. Bradley (1958)
    Sydney, N.S.W. : Periodicals Archive Online (PAO)
    Australasian Journal of Philosophy. 36 (1958) 46 
    Details
    ISSN: 0004-8402
    Thema: Philosophie
    Notizen: DISCUSSION
    URL: http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&res_dat=xri:pao:&rft_dat=xri:pao:article:4251-1958-036-00-000015
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  • 12
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    "On Selfhood and Godhood" (A. Boyce Gibson) (Book Review) (1959)
    Sydney, N.S.W. : Periodicals Archive Online (PAO)
    Australasian Journal of Philosophy. 37 (1959) 242 
    Details
    ISSN: 0004-8402
    Thema: Philosophie
    Notizen: CRITICAL NOTICES
    URL: http://gateway.proquest.com/openurl?url_ver=Z39.88-2004&res_dat=xri:pao:&rft_dat=xri:pao:article:4251-1959-037-00-000024
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  • 13
    Digitale Medien
    Digitale Medien
    Particle-mediated gene transfer to rat neurons in primary culture (2000)
    Springer
    Pflügers Archiv 439 (2000), S. 730-738 
    Details
    ISSN: 1432-2013
    Schlagwort(e): Dorsal root ganglion neurons Gene gun Green fluorescent protein Hippocampal neurons Particle-mediated gene transfer Primary tissue culture Transfection
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract. Gene transfer into neuronal cells provides an important approach to study their function. Particle-mediated gene delivery was used to transfect rat dorsal root ganglion (DRG) and hippocampal neurons in primary culture with the genes for the enhanced blue and green fluorescent proteins (EBFP and EGFP) under control of the cytomegalovirus promoter. Quantitative analysis of marker protein fluorescence detected expression at 3 h that continued to increase for 48 h. For DRG neurons the optimal expression efficiency of 8±2% was obtained 24 h following transfection. In contrast, approximately 2±1% of hippocampal neurons in culture expressed EGFP at 3 h which subsequently declined. Co-transfection of DRG cultures with two plasmids produced reliable expression of both genes. Transfected DRG neurons exhibited normal electrophysiological properties, and resting and stimulated intracellular Ca2+ concentrations were unchanged. After transfection, 44% of hippocampal neurons remained in functional synaptic networks as indicated by glutamatergic Ca2+ spiking activity. Particle-mediated gene delivery provided a straightforward, reproducible and efficient method for transfection of neurons in primary culture. Transfected cells were easily identified by EGFP fluorescence, enabling subsequent physiological analysis. Biolistic particle bombardment was well tolerated by peripheral neurons, although caution was required when this method was applied to CNS cultures.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s004249900240
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  • 14
    Digitale Medien
    Digitale Medien
    A first estimate of organic carbon storage in Holocene lake sediments in Alberta, Canada (2000)
    Springer
    Journal of paleolimnology 24 (2000), S. 395-400 
    Details
    ISSN: 1573-0417
    Schlagwort(e): carbon storage ; lake sediment ; Holocene ; Canada ; climate change ; organic matter
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Geologie und Paläontologie
    Notizen: Abstract This paper reports a first estimate of the Holocene lake sediment carbon pool in Alberta, Canada. The organic matter content of lake sediment does not appear to depend strongly on lake size or other limnological parameters, allowing a simple first estimate in which we assume all Alberta lake sediment to have the same organic matter content. Alberta lake sediments sequester about 15 g C m-2 yr-1, for a provincial total of 0.23 Tg C yr-1, or 2.3 Pg C over the Holocene. Alberta lakes may represent as much as 1/1700 of total global, annual permanent carbon sequestration.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1023/A:1008103605817
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  • 15
    Digitale Medien
    Digitale Medien
    Microbial Biomass and Community Structure in a Sequence of Soils with Increasing Fertility and Changing Land Use (2000)
    Springer
    Microbial ecology 40 (2000), S. 223-237 
    Details
    ISSN: 1432-184X
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The microbial biomass and community structure of eight Chinese red soils with different fertility and land use history was investigated. Two community based microbiological measurements, namely, community level physiological profiling (CLPP) using Biolog sole C source utilization tests and phospholipid fatty acid (PLFA) profiles, were used to investigate the microbial ecology of these soils and to determine how land use alters microbial community structure. Microbial biomass-C and total PLFAs were closely correlated to organic carbon and total nitrogen, indicating that these soil microbial measures are potentially good indices of soil fertility in these highly weathered soils. Metabolic quotients and C source utilization were not correlated with organic carbon or microbial biomass. Multivariate analysis of sole carbon source utilization patterns and PLFAs demonstrated that land use history and plant cover type had a significant impact on microbial community structure. PLFAs showed these differences more than CLPP methods. Consequently, PLFA analysis was a better method for assessing broad-spectrum community differences and at the same time attempting to correlate changes with soil fertility. Soils from tea orchards were particularly distinctive in their CLPP. A modified CLPP method, using absorbance readings at 405 nm and different culture media at pH values of 4.7 and 7.0, showed that the discrimination obtained can be influenced by the culture conditions. This method was used to show that the distinctive microbial community structure in tea orchard soils was not, however, due to differences in pH alone.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s002480000053
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  • 16
    Digitale Medien
    Digitale Medien
    I. Ueber Azimidoverbindungen (1889)
    Weinheim : Wiley-Blackwell
    Liebigs Annalen 255 (1889), S. 339-356 
    Details
    ISSN: 0075-4617
    Schlagwort(e): Chemistry ; Organic Chemistry
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Chemie und Pharmazie
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1002/jlac.18892550307
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