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  • 11
    Electronic Resource
    Electronic Resource
    Springer
    Cellular and molecular life sciences 39 (1983), S. 1335-1338 
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Several blood-sucking arthropods, after a blood meal, are able to store the hemoglobin from their hosts in a crystalline state in their digestive system5,15,20. Guinea-pig hemoglobin crystallizes in the stomach of the reduviid bugRhodnius prolixus in two different crystal types. We show them to be crystallographically identical and to contain the same liganded state of hemoglobin, i.e. they represent different habits of the same crystal modification. The hemoglobin crystallizes in oxy-form and ages in the crystalline state, first to aquomethemoglobin and subsequently to hemichrome without crystal cracking. The rate of aging appears to be the same for both types. The hemoglobin crystal modification observed in the digestive system ofRhodnius prolixus is highly host- but not parasite- specific. The same modification is also observed in vitro and inOrnithodorus moubata, an arachnid whose digestive system differs considerably from that of the insectRhodnius. The retainment period of the crystals represents a long term host-record of possible medical interest.
    Type of Medium: Electronic Resource
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  • 12
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 266 (1977), S. 536-538 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] O. moubata porcinus ticks (males and females) were taken from the laboratory strain, fed on guinea-pigs and dissected at intervals after the blood meal (0, 2, 4, 6, 12, 15, 30, 85 and 200 d), in 0.7% NaCl solution (for X-ray diffraction and spectroscopy) or without a dissecting fluid (for ...
    Type of Medium: Electronic Resource
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  • 13
    ISSN: 1432-0584
    Keywords: Plasma cell leukemia ; LCL ; EBV
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Two long-term cell lines were established in vitro from the peripheral blood of a patient with plasma cell leukemia: one line with plasma cell proliferation, the other with lymphoblastoid cell proliferation (LCL). The 9-month-old plasma cell line showed the typical morphology of plasmoblasts. The cells neither had B-nor T-lymphocyte characteristics, were EBV negative, and showed aneuploidy with various marker chromosomes, including the 14 q+ marker. The cytogenetic findings indicate a monoclonal proliferation of the plasmacells. No tumor growth in thymusless nude mice could be induced upon intracranial inoculation with these cells. In contrast, the autologous LCL, cultured after addition of exogenous EBV, showed the characteristic markers of lymphoblastoid cells, with the typical morphology of pear and handmirror-shaped lymphoblasts, growing in clumps. They had C3- and Fc-receptors, surface-Ig, E-rosette-negativity, a diploid karyotype, and EBV dependent macromolecule synthesis. The lymphoblastoid cells produced intracranial tumors in nude mice in 8 out of 8 attempts.
    Type of Medium: Electronic Resource
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  • 14
    Electronic Resource
    Electronic Resource
    Springer
    Archives of orthopaedic and trauma surgery 78 (1974), S. 199-215 
    ISSN: 1434-3916
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Metallimplantate (Osteosynthesematerial) aus Stahl und/oder Titan mit unterschiedlicher Oberflächenbehandlung wurden im Tierexperiment (Schweizer Bergschafe) auf ihre Gewebeverträglichkeit untersucht. Der Einfluß der Metallimplantate auf das umgebende Bindegewebe ist die Resultante aus: a) Elektrochemisches Milieu auf Grund der Metallkombination oder der Metalloberfläche. Die Oberflächenfeinstruktur der Implantate wurde mit Hilfe des Rasterelektronenmikroskopes untersucht. Sie ist bei den Stahlimplantaten, abgesehen von Korrosionsstellen, relative glatt und bei den oberflächenbehandelten Titanimplantaten orangenhautähnlich. b) Menge und Art der Metallpartikel, die sich von der Implantatoberfläche ablösen. Eine Antwort darauf gibt die Röntgenmikroanalyse. Die Bindegewebeumgebung der Stahlimplantate enthält die chtotoxischen Elemente Eisen und Chrom, die Umgebung der Titanimplantate dazu das gewebefreundliche Titan. c) Cytotoxicität der Metallpartikel. Sie wurde mit Hilfe einer morphometrischen Analyse des metallumgebenden Bindegewebes analysiert. Die verschiedenen Stahlkombinationen weisen, verglichen mit den Titankombinationen, die größere Zellreduktion im Bindegewebe auf und haben folglich auch die größere Cytotoxicität. Diese Tatsache drückt sich auch im Verhältnis der Zellzahl und der Kernanschnittsfläche aus. Diese Proportionalität wird bei den Stahlkombinationen durch eine kleinere Anzahl größerer Zellkerne und bei den Titankombinationen durch eine größere Anzahl kleinerer Zellkerne in einem konstanten Verhältnis von 1∶10 gehalten. Dies bedeutet, daß Reiz und Reizbeantwortung, wie sie durch die Metallimplantate ausgelöst werden, bei den Stahlkombinationen am größten sind.
    Notes: Summary The tissue compatibility of titanium and steel implants of different surface treatment was studied in animal experiments. The reaction of connective tissue surrounding metallic implants is the resultant of the following factors: a) Local currents due to combination of metallic material or metal surface. In the case of steel implants the ultrastructural surface studied with the aid of scanning electron microscopy is smooth, apart from some corrosions spots. In the case of surface treated titanium implants, the surface is orange peel like. b) Quantity and kind of metallic particles detached from the implant surface were studied by electron probe X-ray microanalysis. The connective tissue surrounding the steel implants contains the cytotoxic elements iron and chronium. In addition to this, the environment of the titanic implants shows titanium of good tissue affinity. c) The cytotoxicity of the particles was studied by the morphometric analysis of the metalsurrounding connective tissue. In comparison with the titanium combinations, the steel combinations show a stronger cell reduction in the connective tissue and consequently, a higher cytotoxicity. This fact is also reflected in the ratio between cell number and nuclear size. In the case of steel combinations, this proportion is maintained in a constant ratio of 1∶10 due to a smaller number of larger cell nuclei; in the case of titanium combinations due to a higher number of smaller cell nuclei. This means that stimulus and response to stimulus, such as produced by metallic implants, is most intensive in the case of steel combinations.
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  • 15
    Electronic Resource
    Electronic Resource
    Springer
    Graefe's archive for clinical and experimental ophthalmology 207 (1978), S. 229-242 
    ISSN: 1435-702X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Es werden die rasterelektronenmikroskopischen Befunde von 16 epiretinalen Fibroplasien mit Netzhautfältelung nach verschiedenen primären Funduserkrankungen dargestellt. In diesem ersten Teil besprechen die Autoren: 1. Fibröse, zellfreie epiretinale Membranen mit und ohne strangförmige Glaskörperadhärenzen. Die Fältelung der Retina kommt durch Glaskörpertraktion und Faserbrücken zustande. 2. Fibröse Membranen, welchen einzelne Gliazellen ein- und aufgelagert sind, ohne daß Zellmembranen entstehen. Auch hier wird die Fältelung durch Kollagenfaserbrücken verursacht. 3. Fibröse Membranen, denen gebietsweise Gliamembranen aufgelagert sind. Die Fasermembranen aller drei Typen werden als residuale Glaskörperrinde interpretiert. Weil sie über allen Faltenherden vorhanden sind und wegen ihrem Verhalten zu den Limitansfalten wird angenommen, daß ihre Kontraktion Ursache der Netzhautfältelung ist. Morphologische Anhaltspunkte für Kontraktion von Gliamembranen oder Faserbildung durch die Glia wurden nicht gefunden.
    Notes: Summary The scanning electron microscope was used to examine 16 cases of epiretinal membrane formation and retinal puckers which occurred in a variety of primary retinal disorders. In this first section the authors describe three types of epiretinal membranes: (1) Fibrous acellular membranes with and without vitreous adhesions. Retinal puckering was caused by vitreous traction with collagen strands binding the residual folds. (2) Fibrous membranes containing isolated glial cells. (3) Fibrous membranes partly covered by sheets of glial cells. In all three forms the acellular fibrous component is believed to represent vitreous cortex remnants. The invariable presence of these fibrous membranes at sites of retinal pucker and their relationship to wrinkled internal limiting membrane suggests that contraction of these membranes is responsible for retinal puckering. Morphological evidence of glial membrane contraction and collagen production by glial cells was not found.
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  • 16
    Electronic Resource
    Electronic Resource
    Springer
    Graefe's archive for clinical and experimental ophthalmology 204 (1977), S. 19-29 
    ISSN: 1435-702X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Die Autoren beschreiben die rasterelektronenmikroskopischen Befunde der Netzhautoberfläche nach sekundärer und seniler hinterer Glaskörperabhebung und korrelieren sie mit den Befunden der konventionellen Elektronenmikroskopie. Auf der Netzhautinnenfläche lassen sich nach Abhebung des Glaskörpers noch ausgedehnte Netze von Glaskörperfibrillen finden, deren Fasern ein in sich geschlossenes Filzwerk bilden. Weder Biomikroskopie noch Bulbusdissektion können solche Faserresiduen nachweisen. Diese werden lokalisiert und flächig abgebaut, bis die Basalmembran der Müllerzellen entblößt ist. Auf strukturelle Unterschiede bedingt durch die beiden Untersuchungstechniken und Artefaktmöglichkeiten wird hingewiesen.
    Notes: Summary The authors describe their findings by scanning electron microscopy of the surface of the retina following secondary and primary senile posterior vitreous detachment. These findings are correlated with those of transmission electron microscopy. Extended nets formed by vitreous fibrils can be found on the retinal surface. The fibrils form a continous feltwork, which cannot be traced by biomicroscopy or dissection of enucleated globes. This feltwork desintegrates in places or over a wide area and the basal membrane of the Müller cells becomes exposed. Structural differences in consequence of the two examination techniques well as possible artifacts are shown.
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  • 17
    Electronic Resource
    Electronic Resource
    Springer
    Graefe's archive for clinical and experimental ophthalmology 204 (1977), S. 31-37 
    ISSN: 1435-702X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary The findings by scanning and transmission electron microscopy of multiple glial excrescences from the retina into the cortical vitreous body are described. These formations are located in lines above superficial retinal vessels. The findings are compared with those from light microscopy of similar formations. The pathogenesis and the possible significance as retinovitreal attachments for rhegmatogenous defects of the retina and for posterior vitreous detachments are discussed.
    Notes: Zusammenfassung Es werden die rasterelektronenmikroskopischen Befunde und die elektronenmikroskopischen Schnittbilder von multiplen, über oberflächlichen Netzhautgefäßen gelegenen Gliaauswüchsen aus der Netzhaut in die Glaskörperrinde beschrieben. Diese Befunde werden mit lichtmikroskopischen Bildern ähnlicher Bildungen verglichen, ihre Pathogenese diskutiert und ihre mögliche Bedeutung als vitreoretinale Adhärenzen für rhegmatogene Netzhautdefektbildungen und hintere Glaskörperabhebung erörtert.
    Type of Medium: Electronic Resource
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  • 18
    ISSN: 1420-9071
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Various methods of fixation ofDermatophagoides pteronyssinus for the REM were tested. OsO4 vapor yielded the best results. The morphology of male and female mites is described, based on REM exposures.
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  • 19
    Electronic Resource
    Electronic Resource
    Springer
    Graefe's archive for clinical and experimental ophthalmology 201 (1976), S. 29-38 
    ISSN: 1435-702X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Es werden die rasterelektronenmikroskopischen Befunde an drei Netzhautrundlöchern beschrieben. Man findet eine aus den Foramina auf die Netzhautinnenfläche auswachsende Gliamembran mit mikrovillöser Oberfläche. Diese Zellmembran schiebt sich auf einen Kollagenfaserfilz der umgebenden vitreoretinalen Grenzschicht. Rudimente von Sinneszellrezeptoren stehen an den tiefen Lochrändern. Die Schicht der Pigmentepithelien unter den Foramina ist durch exzessiven Microvillibesatz ausgezeichnet.
    Notes: Summary The findings by scanning electron microscopy on three retinal holes are described. An outgrow of a glial membrane with microvilli from the holes to the retinal surface can be seen. This epiretinal cell membrane grows on a felt of collagen fibres of the vitreoretinal borderlayer. Rudiments of visual receptors project from the deep walls into the holes. The layer of the pigment epithelial cells beneath the holes is remarkable by the abundance of microvilli.
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  • 20
    Electronic Resource
    Electronic Resource
    Springer
    Graefe's archive for clinical and experimental ophthalmology 210 (1979), S. 109-120 
    ISSN: 1435-702X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Es werden die licht- und rasterelektronenmikroskopischen Befunde von drei pigmentierten epiretinalen Fibroplasien eines amotiooperierten Auges beschrieben. Es handelt sich um fibro-gliöse Schrumpffaltenherde, deren Epizentrum von einer Schicht aus retinalem Pigmentepithel überlagert wird. Die Zellen zeigen die Oberflächenstrukturen typischer oder leicht degenerativ veränderter Epithelien. Ausgedehnte Gebiete dieser Pigmentepithelmembranen sind pigmentfrei. Anhaltspunkte für direkte oder indirekte Traktion des Pigmentepithels auf die Netzhautinnenfläche liegen nicht vor. Die Herkunft der Zellen und ihre Bedeutung für die Faltenbildung werden diskutiert.
    Notes: Summary The light- and scanning electron microscopic appearance of 3 areas of pigmented epiretinal fibroplasia (pucker) are described in an eye removed following detachment surgery. The centre of each area of pucker consists of a fibroglial sheet covered by retinal pigment epithelium. The surface morphology of these cells resembles typical or slightly degenerated epithelium. Extensive areas of these pigment epithelial sheets were pigmentfree. There was no evidence that the pigment epithelial sheets exerted either direct or indirect traction on the inner surface of the retina. The origin of these cells and their role in the formation of retinal wrinkling is discussed.
    Type of Medium: Electronic Resource
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