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  • 11
    Electronic Resource
    Electronic Resource
    Studies on interdomain interaction of 3-isopropylmalate dehydrogenase from an extreme thermophile, Thermus thermophilus, by constructing chimeric enzymes (1999)
    Springer
    Extremophiles 3 (1999), S. 259-262 
    Details
    ISSN: 1433-4909
    Keywords: Key words Thermophilic enzymes ; 3-Isopropylmalate dehydrogenase ; Thermostability ; Thermus thermophilus ; Chimeric proteins ; Interdomain interaction ; Differential scanning calorimetry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In our previous study, we showed that a chimeric isopropylmalate dehydrogenase, 2T2M6T, between an extreme thermophile, Thermus thermophilus, and a mesophile, Bacillus subtilis, isopropylmalate dehydrogenases (the name roughly denotes the primary structure; the first 20% from the N-terminal is coded by the thermophile leuB gene, next 20% by mesophile, and the rest by the thermophile gene) denatured in two steps with a stable intermediate, suggesting that in the chimera some of the interdomain interaction was lost by amino acid substitutions in the "2M" part. To identify the residues involved in the interdomain interactions, the first and the second halves of the 2M part of the chimera were substituted with the corresponding sequence of the thermophile enzyme. Both chimeras, 3T1M6T and 2T1M7T, apparently showed one transition in the thermal denaturation without any stable intermediate state, suggesting that the cooperativity of the conformational stability was at least partly restored by the substitutions. The present study also suggested involvement of one or more basic residues in the unusual stability of the thermophile enzyme.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s007920050125
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  • 12
    Electronic Resource
    Electronic Resource
    Molecular characterization of morphologically typical human calicivirus Sapporo (1997)
    Springer
    Archives of virology 142 (1997), S. 1537-1552 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary. Human calicivirus Sapporo (SV) has typical calicivirus morphology and causes acute gastroenteritis in children. The nucleotide sequence of 3.2 kb of the 3′ end of SV was determined from a cloned cDNA. The 3′ end of the SV genome is predicted to encode the RNA-dependent RNA polymerase region, the capsid protein and two small open reading frames. The nonstructural and capsid protein coding sequences in the SV genome are fused in a single open reading frame. The organization of these proteins in the SV sequence is similar to that of rabbit hemorrhagic disease virus and the recently described Manchester virus, and distinct from the genome organization of the prototype human calicivirus, Norwalk virus, that lacks typical calicivirus morphology and has been described as a small round structured virus (SRSV). Sequence analysis of the predicted capsid region showed that the SV capsid is longer by ∼30 amino acids than the capsid of any of the SRSVs, and multiple sequence alignments showed that these additional amino acids are located in the variable region of the capsid protein. Expression of the capsid protein of SV in insect cells resulted in the self-assembly of virus-like particles that have a morphology similar to that of the native virus. This result shows that calicivirus morphology is determined by the primary sequence of the capsid protein.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s007050050178
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  • 13
    Electronic Resource
    Electronic Resource
    Dot blot hybridization with a cDNA probe derived from the human calicivirus Sapporo 1982 strain (1996)
    Springer
    Archives of virology 141 (1996), S. 1949-1959 
    Details
    ISSN: 1432-8798
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A dot blot hybridization assay was developed for detection of human calicivirus/Sapporo/82/J (HuCV/Sa/82) or strains closely related to HuCV/Sa/82 in stool specimens. The cDNA derived from the RNA-dependent RNA polymerase (RDRP) region of HuCV/Sa/82 was used as a positive probe and the pBR 322 DNA as a negative control probe. Both probes were labeled with digoxigenin and the products of hybridization reaction were detected with an anti-digoxigenin antibody-alkaline phosphatase conjugate. This assay was specific for HuCV/Sa/82 and for HuCV antigenically related to HuCV/Sa/82. The lower limit of sensitivity of this assay was estimated to be about 105 physical particles or 10 pg of cDNA, similar to that of the previously developed ELISA for HuCV. In 1 273 stool specimens obtained from children with acute gastroenteritis in Sapporo, Japan, 110 (8.6%) contained small round structured viruses by EM and 23 (1.8%) were positive for HuCV antigenically related to HuCV/Sa/82 by either the hybridization assay or ELISA. A higher positive rate was obtained with the dot blot assay (21%) than by ELISA (10%), suggesting that the dot blot assay either detects HuCV more broadly than the ELISA or detects HuCV covered with fecal antibodies which interrupt antigen-antibody reactions in the ELISA. Negative results for detection of Norwalk virus (NV) cDNA and feline calicivirus (FCV) RNA by both this assay and the ELISA indicated that the HuCV/Sa/82 strain is distinct antigenically and genetically from NV and FCV.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01718206
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  • 14
    Electronic Resource
    Electronic Resource
    The constraints of non-standardWWγ couplings from FCNC processes (1991)
    Springer
    The European physical journal 52 (1991), S. 691-700 
    Details
    ISSN: 1434-6052
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract It was reported that the contributions of the non-standardWWγ couplings called λγ and $$\bar \lambda _\gamma $$ to the anomalous magnetic moments and the electric dipole moments of fermions depend on regularization schemes of the loop integral. We find that this dependence does not exist in the contributions of the λγ and $$\bar \lambda _\gamma $$ couplings to the flavor changing neutral current (FCNC) processes of quarks such asb→sγ. By utilizing the experimental upper bound of the branching ratio of the processB→K *γ, we obtain the allowed regions of λγ and $$\bar \lambda _\gamma $$ that do not depend on regularization schemes of the loop integral. The cut-off independent constraints of the other non-standardWWγ couplings called Δκλ and $$\tilde \kappa _\gamma $$ are also obtained.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01562346
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