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  • Rhizobium  (3)
  • Calibrator material  (1)
  • 1
    Digitale Medien
    Digitale Medien
    Delivering quality for the measurement of immunosuppressive drugs: current performance and future needs (1999)
    Springer
    Accreditation and quality assurance 4 (1999), S. 427-430 
    Details
    ISSN: 1432-0517
    Schlagwort(e): Key words Drugs proficiency testing ; Analytical quality indicators ; Calibrator material ; Traceability
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Abstract  Whilst it is generally agreed that measurement of immunosuppressive drugs is of value as a guide to therapy, there are a number of problems associated with the analytical techniques available. Proficiency testing schemes enable laboratories to judge their performance for the measurement of these drugs with their peers. However, current schemes only compare accuracy within-methods to document performance. This paper summarizes current findings and points out the limitations of our own schemes, and the measures we intend to use to address these deficiencies.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s007690050402
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  • 2
    Digitale Medien
    Digitale Medien
    Appearance of a novel form of plant glutamine synthetase during nodule development in Phaseolus vulgaris L. (1983)
    Springer
    Planta 157 (1983), S. 254-258 
    Details
    ISSN: 1432-2048
    Schlagwort(e): Glutamine synthetase ; Leghaemoglobin ; Nitrogenase ; Nitrogen fixation ; Phaseolus ; Rhizobium
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The activities of glutamine synthetase (GS), nitrogenase and leghaemoglobin were measured during nodule development in Phaseolus vulgaris infected with wild-type or two non-fixing (Fix-) mutants of Rhizobium phaseoli. The large increase in GS activity which was observed during nodulation with the wild-type rhizobial strain occurred concomitantly with the detection and increase in activity of nitrogenase and the amount of leghaemoglobin. Moreover, this increase in GS was found to be due entirely to the appearance of a novel form of the enzyme (GSn1) in the nodule. The activity of the form (GSn2) similar to the root enzyme (GSr) remained constant throughout the experiment. In nodules produced by infection with the two mutant strains of Rhizobium phaseoli (JL15 and JL19) only trace amounts of GSn1 and leghaemoglobin were detected.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00405190
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  • 3
    Digitale Medien
    Digitale Medien
    A mutation that blocks exopolysaccharide synthesis prevents nodulation of peas by Rhizobium leguminosarum but not of beans by R. phaseoli and is corrected by cloned DNA from Rhizobium or the phytopathogen Xanthomonas (1986)
    Springer
    Molecular genetics and genomics 203 (1986), S. 320-323 
    Details
    ISSN: 1617-4623
    Schlagwort(e): Exopolysaccharide ; Legume ; Nodules ; Rhizobium ; Xanthomonas
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary A Tn5-induced mutant strain of R. phaseoli which failed to synthesize exopolysaccharide (EPS) was isolated and was shown to induce normal nitrogen-fixing nodules on Phaseolus beans, the host of this Rhizobium species. The corresponding wild-type Rhizobium DNA was cloned in a wide host-range vector and by isolating Tn5 insertions in this cloned DNA, mutations in a gene termed pss (polysaccharide synthesis) were isolated. These were introduced by marker exchange into near-isogenic strains of R. leguminosarum and R. phaseoli which differed only in the identity of their symbiotic plasmids. Whereas the EPS-deficient mutant strain of R. phaseoli induced normal nitrogen-fixing nodules on Phaseolus beans, the same mutation prevented nodulation of peas by a strain of R. leguminosarum which normally nodulates this host. Further, it was found that DNA cloned from the plant pathogen Xanthomonas campestris pathover campestris could correct the defect in EPS synthesis in R. leguminosarum and R. phaseoli and also restored the ability to nodulate peas to the pss::Tn5 mutant strain of R. leguminosarum.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00333974
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  • 4
    Digitale Medien
    Digitale Medien
    Sequence of psi, a gene on the symbiotic plasmid of Rhizobium phaseoli which inhibits exopolysaccharide synthesis and nodulation and demonstration that its transcription is inhibited by psr, another gene on the symbiotic plasmid (1987)
    Springer
    Molecular genetics and genomics 207 (1987), S. 149-154 
    Details
    ISSN: 1617-4623
    Schlagwort(e): Beans ; DNA Sequence ; Gene regulation ; Nitrogen fixation ; Nodulation ; Rhizobium
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary A gene termed psi (polysaccharide inhibition), located close to the nodulation genes of the Rhizobium phaseoli symbiotic plasmid pRP2JI inhibited exopolysaccharide synthesis (EPS) and nodulation ability (Nod) in Rhizobium when it was cloned in a multicopy plasmid. The sequence of psi showed that it specified a polypeptide of mol. wt. 10000 that may be associated with the membrane of Rhizobium. A second gene, psr (polysaccharide restoration), was located on pRP2JI. When cloned in multicopy plasmids, psr overcame the EPS and Nod defects in strains carrying multicopy psi. Strains with multicopy psr induced non-fixing nodules on Phaseolus beans. Using gene fusions between psi and lacZ, it was shown that psi inhibited transcription of psr.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00331502
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