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  • Electron microscopy  (4)
  • Circumventricular organs  (3)
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Materialart
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  • 1
    Digitale Medien
    Digitale Medien
    Serotonin distribution in the circumventricular organs of the rat (1983)
    Springer
    Anatomy and embryology 167 (1983), S. 311-319 
    Details
    ISSN: 1432-0568
    Schlagwort(e): Serotonin ; Circumventricular organs ; Rat ; Immunohistochemistry
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Summary Serotonin-immunoreactive structures in the circumventricular organs (organum vasculosum laminae terminalis, subfornical organ, subcommissural organ and area postrema) of the rat were demonstrated using a modified peroxidase-antiperoxidase immunohistochemical method. Various densities of serotonin fibers were demonstrated in all four circumventricular organs; however, serotonin-positive cells were evident in the area postrema only after nialamide treatment. Serotonergic supraepedymal fibers were observed on the surface of the organum vasculosum laminae terminalis and that of the subfornical organ, but not on the subcommissural organ and area postrema. The serotonergic plexus of the basal portion of the subcommisural organ was considered to be continuous with the supraependymal plexus.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00315669
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  • 2
    Digitale Medien
    Digitale Medien
    Electron microscopy of the arcuate nucleus of normal and 5-hydroxydopamine treated cats (1975)
    Springer
    Cell & tissue research 160 (1975), S. 139-153 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Arcuate nucleus, cat ; Dense-cored vesicles ; Dopamine ; 5-Hydroxydopamine treatment ; Electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The arcuate nucleus of normal cats and of cats treated with 5-hydroxydopamine (5-OHDA) was investigated by electron microscopy. The neurons of the arcuate nucleus were classified into three types, clear, intermediate and dark, according to their fine structure. The clear type contained numerous dense-cored vesicles and well developed cell organelles. All three types were frequently seen to be partially surrounded by glial processes. Many axo-somatic and axo-dendritic synapses mostly small in diameter were also observed around the neurons. Synaptic contacts were demonstrated between axon endings and axonal processes which contained elementary granules. After administration of 5-OHDA small and large dense-cored vesicles appeared in the nerve endings surrounding the neurons. The relationship between the dense-cored vesicles in the perikarya and dopamine was briefly discussed.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00220574
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  • 3
    Digitale Medien
    Digitale Medien
    The zinc iodide-osmium tetroxide (ZIO) reaction on nerve endings in the median eminence of the rat under normal and experimental conditions (1976)
    Springer
    Cell & tissue research 173 (1976), S. 279-286 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Median eminence ; Rat ; Monoamine ; ZIO reaction ; Electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The reaction of nerve endings in the median eminence of the rat to zinc iodide-osmium tetroxide (ZIO) staining was examined electron microscopically under normal and experimental conditions. The experimental condition of catecholamine exhaustion in the nerve endings was induced by the administration of H44/68 and reserpine. Vesicles in the terminals of catecholaminergic nerves reacted similarly to ZIO staining in both normal and experimental material. The majority of synaptic vesicles in various terminals gave a positive ZIO reaction. The neurosecretory elementary granules, however, failed to react with ZIO. On the other hand, some nerve terminals in the external layer of the median eminence showed a strong positive reaction in the cytoplasmic matrix, in mitochondria as well as in synaptic vesicles. These findings strongly suggest that the ZIO-positive substance in nerve terminals is not the transmitter itself, i.e. the monoamine, but rather represents a range of substances commonly found in various kinds of synaptic vesicles and is probably proteinaceous in nature. A brief discussion is also given on the difference in ZIO reactivity between neurosecretory elementary granules and small vesicles in the hypothalamo-hypophyseal tract.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00220316
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  • 4
    Digitale Medien
    Digitale Medien
    The sympathetic innervation of the ciliary body and trabecular meshwork of the cat (1976)
    Springer
    Cell & tissue research 173 (1976), S. 261-269 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Cat ; Ciliary body ; ‘False’ transmitters ; Fluorescence histochemistry ; Electron microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The ciliary body of the cat was investigated by fluorescence histochemistry and electron microscopy in an attempt to clarify its sympathetic innervation. Subconjunctival doses of 5-hydroxydopamine (5-OHDA) or 6-hydroxydopamine (6-OHDA) were given to establish the precise location of the sympathetic nerve terminals. The distribution of noradrenergic fibers and terminals was shown by fluorescence histochemistry to be sparse in the trabecular meshwork and the anterior portion of the ciliary muscle, but dense in the subepithelial tissue. The small and large dense core vesicles which occur in many nerve endings of the subepithelial tissue adjacent to the pigmented epithelial layer increased in electron density following the administration of 5-OHDA. Many degenerating nerve endings were found in the same region of animals treated with 6-OHDA. In contrast, there were few noradrenergic terminals in the ciliary muscle except for a portion of the smooth muscle which was shown to be dually innervated. The noradrenergic fibers in the subepithelial region and the trabecular meshwork may play an important role in aqueous secretion and outflow.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00221379
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  • 5
    Digitale Medien
    Digitale Medien
    CRF-immunoreactive nerve fibers in the circumventricular organs of the monkey, Macaca fuscata (1983)
    Springer
    Cell & tissue research 232 (1983), S. 679-683 
    Details
    ISSN: 1432-0878
    Schlagwort(e): CRF-immunoreactive nerve fibers ; Circumventricular organs ; Immunohistochemistry ; Monkey, Macaca fuscata
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The occurrence of CRF (corticotropin-releasing factor)-immunoreactive nerve fibers in the circumventricular organs of adult male monkeys, Macaca fuscata, was studied on serially sectioned brains, by means of the peroxidase-antiperoxidase technique in combination with a highly specific and sensitive CRF antiserum. CRF-containing nerve fibers were found in high concentrations in the infundibulum and, in addition, in small numbers in the posterior lobe, organum vasculosum laminae terminalis, subfornical organ, and area postrema; they were missing in the pineal body and the subcommissural organ. The CRF immunoreactive nerve fibers distributed in these organs were located in the proximity of the blood vessels.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00216438
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  • 6
    Digitale Medien
    Digitale Medien
    Morphological studies on neuroglia (1981)
    Springer
    Cell & tissue research 216 (1981), S. 557-568 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Electron microscopy ; Neuroglia ; Silver impregnation ; Brain ; Cat
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The silver-impregnation procedure of Tsujiyama is suitable for demonstration of all three classical types of neuroglial cells; in the present study it was used for electron microscopic identification of neuroglial cells in the brain of the cat. The aim of the present study was 1) to determine impregnated structural correlates of neuroglial cells at the light- and electron-microscopic levels, and 2) to determine whether the method of Tsujiyama is applicable for the electron microscopic identification of the single types of neuroglial cells. Silver deposits were observed over the cytoplasm and processes of astrocytes where numerous glial filaments were present. Oligodendrocytes and microglial cells may be precisely differentiated by use of Tsujiyama's silver impregnation method at the electron microscopic level due to the pattern of silver-deposition in these two basic types of cells. This silver-impregnation method combined with electron microscopy is thus suitable for a precise identification of neuroglial cells; the technique may prove to be very helpful in identification of such categories of neuroglial cells that encompass also the images of cells which cannot be classified by use of the standard methods.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00238651
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  • 7
    Digitale Medien
    Digitale Medien
    Cells capable of uptake of horseradish peroxidase in some circumventricular organs of the cat and rat (1981)
    Springer
    Cell & tissue research 219 (1981), S. 85-92 
    Details
    ISSN: 1432-0878
    Schlagwort(e): Circumventricular organs ; Reticuloendothelial system ; Phagocytic cells ; Horseradish peroxidase ; Cat ; Rat
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The structure of mesenchymal cells distributed in some of the “hypendymal organs” of the circumventricular system in the cat and rat was demonstrated after intravenous injection of high doses of horseradish peroxidase. These cellular elements were observed in the vicinity of blood vessels of the organon vasculosum laminae terminalis, subfornical organ and area postrema. Electron-microscopically, these cells located between the basal laminae of the brain parenchyma and the blood capillaries show long cellular processes encircling fenestrated capillaries. Light and electron-microscopic examination revealed that this cell type is identical with the “horseradish peroxidase-uptake cells”, previously reported in the vicinity of the hypophysial portal system. Such phagocytic cells may be considered as a cellular component intervening between the brain parenchyma and the blood stream, playing a role in selective barrier functions in the above-mentioned circumventricular organs where a blood-brain barrier in the classical sense of the definition is lacking.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF00210020
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