Bibliothek

X
Suchhistorie löschen
feed icon rss

Ihre E-Mail wurde erfolgreich gesendet. Bitte prüfen Sie Ihren Maileingang.

Leider ist ein Fehler beim E-Mail-Versand aufgetreten. Bitte versuchen Sie es erneut.

Vorgang fortführen?

Exportieren
  • 1
    Digitale Medien
    Digitale Medien
    Enhanced cytokine production from human macrophages stimulated by polyethylene particles retrieved from interface membranes after failed total hip arthroplasty (2000)
    Springer
    Modern rheumatology 10 (2000), S. 24-30 
    Details
    ISSN: 1439-7609
    Schlagwort(e): Key words Human monocyte/macrophages ; Ultra-high molecular weight polyethylene (UHMWP) ; Interface membrane tissue ; Total hip arthroplasty (THA)
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract Little is known about the specific effects of the ultra-high molecular weight polyethylene (UHMWP) debris that is obtained from human interface membranes after revision for a failed total hip arthroplasty. This paper reports the effects of retrieved polyethylene particles on human monocyte/macrophages (M/M). Macrophages were prepared from normal human peripheral blood by the conventional Ficoll–Hypaque method. Polyethylene wear debris was obtained from human interface membranes and prepared by the method of papain digestion. Human M/M were dispensed at 1.0 × 106 cells/well in a 24-well culture plate. UHMWP and latex particles were added immediately after plating the cells and directly onto the cells (1 × 106 cells/well) at final particle concentrations of 100 μg/well, 200 μg/well, and 500 μg/well. At the end of 24 h incubation, the culture supernatant was removed and assayed for IL-1β, IL-6, and TNF-α activities by ELISA. Cellular morphology and architecture were studied using light and electron microscopy. Human M/M cultured with retrieved UHMWP particles caused significantly more IL-1β, IL-6, and TNF-α release than macrophages cultured with latex (P 〈 0.05). The addition of latex and polyethylene particles to human M/M resulted in a dose-dependent increase in IL-1β, IL-6, and TNF-α release. Electron microscopy revealed that 90% of the UHMWP particles were less than 1 μm diameter. The average particle size was approximately 0.7 μm diameter (range 0.1–15 μm). Human M/M exposed to PE particles demonstrated extensive filopodia formation as compared with the cells exposed to latex particles. In summary, we have demonstrated that polyethylene particles isolated from interfacial membranes obtained at revision surgery are potent stimulators of human M/M.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s101650070035
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
    BibTip Andere fanden auch interessant ...
    Artikel (Nationallizenzen)
    Volltext
  • 2
    Digitale Medien
    Digitale Medien
    Identification of the proto-oncogene c-fos in areas of periprosthetic osteolysis around loosened hip prostheses (2000)
    Springer
    Modern rheumatology 10 (2000), S. 225-229 
    Details
    ISSN: 1439-7609
    Schlagwort(e): Key words Proto-oncogene c-fos ; Interfacial membrane ; Osteolysis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract Expression of the proto-oncogene c-fos was investigated by in situ hybridization and a study of the immunohistochemistry of the interfacial membranes surrounding the femoral component of failed cementless total hip arthroplasty (THA). The values of the proto-oncogene c-fos gene and protein were high in these interfacial membranes. High expression of the c-fos gene and protein was observed in the macrophage-like cells and fibroblast-like cells. However, there was little expression in multinucleated giant cells. We demonstrated the significance of the proto-oncogene c-fos in interfacial membranes – the first report of c-fos in total joint replacement failure.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/s101650070007
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
    BibTip Andere fanden auch interessant ...
    Artikel (Nationallizenzen)
    Volltext
  • 3
    Digitale Medien
    Digitale Medien
    Anti-transfer RNA antibodies in two patients with pulmonary fibrosis, Raynaud's phenomenon and polyarthritis (1998)
    Springer
    Clinical rheumatology 17 (1998), S. 144-147 
    Details
    ISSN: 1434-9949
    Schlagwort(e): Autoantibody ; Interstitial pulmonary fibrosis ; Polyarthritis ; Raynaud's phenomenon ; Transfer RNA
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Medizin
    Notizen: Abstract Patient W.S. (a 61-year-old woman) and patient T.M. (a 41-year-old man) developed recurrent fevers, polyarthritis, Raynaud's phenomenon and interstitial pulmonary fibrosis without apparent polymyositis. From HeLa cell extracts, sera from both patients immunoprecipitated all species of intact and deproteinised tRNAs. To identify the antibody binding site more precisely, tRNAs transcribed in vitro from clonedEscherichia coli tRNA genes and various mutants were prepared and used as antigens for immunoprecipitation. When the TψC loop, or the D loop were deleted, such mutants were not bound by both sera, suggesting that the D and TψC loops were required for antibody binding. Abrogation of tRNA binding occurred when18G of tRNATrp was replaced with18A to break the tertiary L-shape structure of tRNA. These results strongly suggest that sera from W.S. and T.M. recognise the tertiary conformation of L-shaped tRNA which is constructed with both D and TψC loops. These autoantibodies may also serve as a marker for a new subset of patients with connective tissue diseases that is distinct from anti-aminoacyl-tRNA synthetase syndrome.
    Materialart: Digitale Medien
    URL: http://dx.doi.org/10.1007/BF01452262
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
    BibTip Andere fanden auch interessant ...
    Artikel (Nationallizenzen)
    Volltext
Schließen ⊗
Diese Webseite nutzt Cookies und das Analyse-Tool Matomo. Weitere Informationen finden Sie hier...