ISSN:
1432-0428
Keywords:
Galactosamine hepatitis
;
hyperinsulinaemia
;
insulin resistance
;
liver plasma membranes
;
insulin binding
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary Six to 12 h after IP injection of 400 mg/kg of D-galactosamine in rats a 5-fold increase in plasma insulin was observed. In addition, impaired glucose assimilation was present after an IV load in spite of unchanged fasting glucose levels. In streptozotocin-diabetic rats (100 mg/kg IV) plasma insulin remained diminished 12 h after induction of D-galactosamine hepatitis. Under identical conditions of preparation and incubation, the liver plasma membranes of D-galactosamine-treated rats, in both normal and diabetic states, bound only 40–60% as much insulin per mg of membrane protein as those of the control rats. Scatchard analysis suggested that this was due to a decrease in the number of receptor sites in the membranes of the D-galactosamine-injected rats. No difference in the insulin degrading capacity and in insulin-receptor dissociation of the plasma membranes between control and D-galactosaminetreated groups was found. These data suggest that a reduction in the number of hepatic insulin receptors in galactosamine hepatitis can lead to insulin resistance and hyperinsulinaemia.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01222210
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