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  • 1
    Digitale Medien
    Digitale Medien
    Oxford, UK : Blackwell Publishing Ltd
    Ground water 21 (1983), S. 0 
    ISSN: 1745-6584
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Energietechnik , Geologie und Paläontologie
    Notizen: Regions of the earth below the root zone of soil have conventionally been considered void of life. However, widespread pollution of ground water in the U.S. by organic compounds has kindled interest in the numbers of microbes that might be found in aquifers and associated regions of the deeper unsaturated zone, and in their ability to degrade organic pollutants.Newly developed techniques in microbial ecology allow the direct enumeration and examination of soil microbes without recourse to their cultivation on growth media. These techniques reduce many uncertainties associated with the use of culture media, such as the growth of contaminants or the failure of indigenous forms to grow on a particular medium. Samples were recovered aseptically from depths of 1·2, 3·0, and 5·0 meters (m) at the margin of a small floodplain near Lula, Oklahoma. Depth to the water table was 3·6 m; depth to bedrock was 6·0 m. Numbers of microbes were surprisingly similar at all three depths (3 to 9 × 106/g dry material). Although both Gram-positive and Gram-negative bacteria were encountered, small Gram-positive coccoid forms predominated. No protozoa, yeasts, or other fungi were seen. The indigenous bacteria could rapidly degrade toluene. Chlorobenzene was degraded in material from the vadose zone, while bromodichloromethane was degraded in material from the saturated zone. There was no detectable degradation of 1,2-dichloroethane, 1,1,2-trichloroethane, trichloroethylene, or tetrachloroethylene.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Palo Alto, Calif. : Annual Reviews
    Annual Review of Microbiology 38 (1984), S. 515-550 
    ISSN: 0066-4227
    Quelle: Annual Reviews Electronic Back Volume Collection 1932-2001ff
    Thema: Biologie
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    Oxford, UK : Blackwell Science, Ltd
    Journal of fish diseases 22 (1999), S. 0 
    ISSN: 1365-2761
    Quelle: Blackwell Publishing Journal Backfiles 1879-2005
    Thema: Biologie , Medizin
    Notizen: Bacteria were isolated from the diseased eggs of two damselfish (Pomacentridae) species, Amphiprion clarkii and Amblyglyphidodon curacao. Healthy A. clarkii eggs were infected separately with four bacterial isolates from the diseased eggs, as well as with Vibrio parahaemolyticus and Listonella anguillarum. Amphiprion clarkii eggs exposed to Cura-d, an isolate from diseased A. curacao eggs, showed increased mortality compared to uninfected control eggs. The A. curacao eggs were infected separately with the same two isolates from diseased A. curacao eggs, and similarly, eggs exposed to isolate Cura-d also showed increased mortality compared to uninfected eggs. However, the increase in mortality associated with exposure to isolate Cura-d was most significant in A. clarkii eggs. A 16S rDNA comparative sequence analysis identified that isolate Cura-d was most similar to Pseudoalteromonas piscicida, a known fish pathogen. Both Cura-d and P. piscicida were found to be polarly flagellated, Gram-negative rods with an oxidative metabolism which grew on d-mannose, d-fructose, sucrose and maltose, but not on l-threonine. Based on these properties, it is proposed that Cura-d should be classified as P. piscicida strain Cura-d. It is concluded that the P. piscicida strain cura-d was associated with the increased mortality observed in A. clarkii eggs.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 4
    Digitale Medien
    Digitale Medien
    Springer
    Archives of microbiology 123 (1979), S. 213-226 
    ISSN: 1432-072X
    Schlagwort(e): Pedomicrobium ; Budding bacteria ; Iron deposition ; Manganese deposition ; Polymer ; Fine structure
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Morphological characteristics of two Pedomicrobium-like budding bacteria are described. A structured surface layer was regularly observed on strain 868. Ruthenium red- and Alcian blue-staining polymers were found on both strains. When either strain was grown in the presence of iron or manganese, the corresponding oxides accumulated on their surfaces. In thin sections iron oxides appeared as fine threads, arrays of particles or dense coatings, depending on the source of iron. Manganese oxides appeared as branching filaments or convoluted ribbons. Both metal oxides stained with ruthenium red. Extraction of the oxides followed by ruthenium red staining revealed that polyanionic polymers previously deposited on the cells were associated with the metals. Treatment of cultures with glutaraldehyde, HgCl2, or heat, inhibited manganese but not iron deposition, suggesting that iron oxides accumulated by passive, non-biological processes. Manganese oxides apparently accumulated under control of a biological manganese-oxidizing factor. Incomplete inhibition of manganese deposition observed in cell suspensions suggested that, if the oxidizing factor was an enzyme, it was unusually stable. Based on these results, possible mechanisms of iron and manganese deposition in association with extracellular polymers are suggested.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 5
    Digitale Medien
    Digitale Medien
    Springer
    Current microbiology 10 (1984), S. 65-71 
    ISSN: 1432-0991
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Abstract The ultrastructure ofSpiroplasma floricola OBMG was investigated to identify subcellular structures that might be involved in motility and helicity. Optimal preservation for thin sectioning was achieved with either glutaraldehyde or a mixture of glutaraldehyde plus paraformaldehyde followed by OsO4 and uranyl acetate. In thin sections, a 94-nm-wide band consisting of 4-nm-diameter fibrils was observed apposed to the cytoplasmic side of the plasma membrane. The band of fibrils extended axially the entire length of the cell. The addition of rethenium red to fixative solutions resulted in condensation of the fibrils. Freeze-substitution increased the apparent thickness of membranes but did not improve preservation of the fibrils. Freeze-fracturing revealed a 99-nm-wide zone containing few particles in fractured membrane surfaces. Treatment with deoxycholate or Triton X-100 to dissolve membranes yielded bands of fibrils comparable to those seen in thin sections. Based on these findings, a model indicating the intracellular location of the fibrils is proposed.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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