ISSN:
0006-3525
Schlagwort(e):
Chemistry
;
Polymer and Materials Science
Quelle:
Wiley InterScience Backfile Collection 1832-2000
Thema:
Chemie und Pharmazie
Notizen:
The synthetic peptide Gly-L-Ala-L-Val (C10H19N3O4·3H2O; GAV) crystallizes in the monoclinic space group P21, with a = 8.052(2), b = 6.032(2), c = 15.779(7) Å, β = 98.520(1)°, V = 757.8 Å3, Dx = 1.312 g cm-3, and Z = 2. The peptide Gly-L-Ala-L-Leu (C11H21N3O4·3H2O; GAL) crystallizes in the orthorhombic space group P212121, with a = 6.024(1), b = 8.171(1), c = 32.791(1) Å, V = 1614 Å3, Dx = 1.289 g cm-3, and Z = 4. Their crystal structures were solved by direct methods using the program SHELXS-86, and refined to an R index of 0.05 for 1489 reflections for GAV and to an R index of 0.05 for 1563 reflections for GAL. The tripeptides exist as a zwitterion in the crystal and assume a near α-helical backbone conformation with the following torsion angles: ψ1 = -150.7°; φ2, ψ2 = -68.7°, -38.1°; φ3, ψ31, ψ32, = -74.8°, -44.9°, 135.9° for GAV; ψ1 = -150.3°; φ2, ψ2 = -67.7°, -38.9°; φ3, ψ31, ψ32 = -72.2°, -45.3°, 137.5°, for GAL. Both the peptide units in both of the tripeptides show significant deviation from planarity [ω1 = -171.3(6)° and ω2 = -172.0(6)° for GAV; ω1 = -171.9(5)° and ω2 = -173.2(6)° for GAL]. The sidechain conformational angles χ21 and χ22 are -61.7(5)° and 175.7(5)°, respectively, for valine, and the side-chain conformations χ12 and χ23's are -68.5(5)° and (-78.4(6)°, 159.1(5)°) respectively, for leucine. Each of the tripeptide molecule is held in a near helical conformation by a water molecule that bridges the NH3+ and COO- groups, and acts as the fourth residue needed to complete the turn by forming two hydrogen bonds. Two other water molecules form intermolecular hydrogen bonds in stabilizing the helical structure so that the end result is a column of molecules that looks like an α-helix.
Zusätzliches Material:
7 Ill.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1002/bip.360310405
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