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  • Articles: DFG German National Licenses  (9)
  • 1955-1959  (4)
  • 1945-1949  (5)
  • 1910-1914
  • Spektralphotometrie  (5)
  • particle bombardment  (4)
Source
  • Articles: DFG German National Licenses  (9)
Material
Years
Year
Keywords
  • 1
    Electronic Resource
    Electronic Resource
    Transformation studies in Hordeum vulgare using a highly regenerable microspore system (1955)
    Springer
    Euphytica 85 (1955), S. 113-118 
    Details
    ISSN: 1573-5060
    Keywords: Hordeum vulgare ; isolated microspores ; particle bombardment ; transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A highly regenerable, isolated microspore system for barley, Hordeum vulgare L. cv. Igri, has been developed which is amenable to transformation studies using particle bombardment. The system allows DNA to be delivered to microspores at the single cell stage and both transient and stable transformation events have been demonstrated. The potential advantages of using isolated microspores as the target tissue in routine transformation systems are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00023938
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Transgenic barley by particle bombardment. Inheritance of the transferred gene and characteristics of transgenic barley plants (1955)
    Springer
    Euphytica 85 (1955), S. 81-88 
    Details
    ISSN: 1573-5060
    Keywords: gene transfer ; Hordeum vulgare ; neomycin phosphotransferase II ; particle bombardment ; transgenic barley
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Transgenic barley plants (Hordeum vulgare L. cv. Kymppi) were obtained by particle bombardment of various tissues. Immature embryos and microspore-derived cultures were bombarded with gold particles coated with plasmid DNA carrying the gene coding for neomycin phosphotransferase II (NPTII), together with plasmid DNA containing the gene for β-glucuronidase (GUS). Bombarded immature embryos were grown to plants without selection and NPTII activity was screened in small plantlets. One plant proved to be transgenic (T0). This chimeric plant passed the transferred nptII gene to its T1 progeny. The presence of the nptII gene was demonstrated by the PCR technique and enzyme activity was analyzed by an NPTII gel assay. Four T0 spikes and 15 T1 offspring were transgenic. The integration and inheritance was confirmed by Southern blot hybridization. Transgenic T2 and T3 plants were produced by isolating embryos from green grains of transgenic T1 and T2 plants, respectively and growing them to plants. After selfing, the ratio of transgenic to non-transgenic T2 offspring was shown to follow the rule of Mendelian inheritance. The general performance of transgenic plants was normal and no reduction in fertility was observed. Microspore-derived cultures were bombarded one and four weeks after microspore isolation. After bombardment, cultures were grown either with or without antibiotic selection (geneticin R or kanamycin). When cultures were grown without selection and regenerated plants were transferred to kanamycin selection in rooting phase, one out of a total of about 1500 plants survived. This plant both carried and expressed the transferred nptII gene. The integration was confirmed by Southern blot hybridization. This plant was not fertile.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00023933
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Genetic engineering of cereal crop plants: a review (1955)
    Springer
    Euphytica 85 (1955), S. 35-44 
    Details
    ISSN: 1573-5060
    Keywords: cereals ; protoplast transformation ; tissue electroporation ; particle bombardment
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Many aspects of basic and applied problems in plant biology can be investigated by transformation techniques. In dicotyledonous species, the ability to generate transgenic plants provides the tools for an understanding of plant gene function and regulation as well as for the directed transfer of genes of agronomic interest. For many dicotyledonous plants Agrobacterium tumefaciens can be routinely used to introduce foreign DNA into their genome. However, cereals seem to be recalcitrant to Agrobacterium-mediated transformation. In cereals, many efforts have been made in recent years to establish reliable transformation techniques. Several transformation techniques have been developed but to date only three methods have been found to be suitable for obtaining transgenic cereals: transformation of totipotent protoplasts, particle bombardment of regenerable tissues and, more recently, tissue electroporation. The current state of transformation methods used for cereals will be reviewed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00023928
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Strategies for variety-independent genetic transformation of important cereals, legumes and woody species utilizing particle bombardment (1955)
    Springer
    Euphytica 85 (1955), S. 13-27 
    Details
    ISSN: 1573-5060
    Keywords: gene transfer ; crop species ; particle bombardment ; transgenic plants ; cereals ; legumes ; woody plants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The limiting component in the creation of transgenic crops has been the lack of effective means to introduce foreign genes into elite germplasm. However, the development of novel direct DNA transfer methodology, by-passing limitations imposed by Agrobacterium-host specificity and cell culture constraints, has allowed the engineering of almost all major crops, including formerly recalcitrant cereals, legumes and woody species. The creation of transgenic rice, wheat, maize, barley, oat, soybean, phaseolus, peanut, poplar, spruce, cotton and others, in an efficient and in some cases, variety-independent fashion, is a significant step towards the routine application of recombinant DNA methodology to the improvement of most important agronomic crops. In this review we will focus on key elements and advantages of particle bombardment technology in order to evaluate its impact on the accelerated commercialization of products based on agricultural biotechnology and its utility in studying basic plant developmental processes and function through transgenesis. Fundamental differences between conventional gene transfer methods, utilizing Agrobacterium vectors or protoplast/suspension cultures, and particle bombardment will be discussed in depth.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00023926
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    Paper (German National Licenses)
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  • 5
    Electronic Resource
    Electronic Resource
    Photometrische Bestimmung von Benzylhydrazin und Phenelzin (1947)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 265 (1947), S. 325-328 
    Details
    ISSN: 1618-2650
    Keywords: Best. Von Benzylhydrazin und phenelzin ; Spektralphotometrie ; Reaktion mit Glutacondialdehyd
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung Glutacondialdehyd, hergestellt aus Pyridylpyridiniumdichlorid und Natronlauge, reagiert, wenn er im Überschuß vorliegt, in saurer Lösung mit den Monoaminoxydasehemmern Benzylhydrazin und Phenelzin unter Bildung roter Kondensationsprodukte. Diese Farbreaktion bildet die Grundlage der photometrischen Bestimmungsverfahren, bei denen 0,6–100 nMol/ml der Hydrazinderivate bestimmt werden können. Bei 2 nMol/ml betragen die Variationskoeffizienten 3,2 Rel-% für Benzylhydrazin und 5,2 Rel-% für Phenelzin.
    Notes: Abstract Benzylhydraine and phenelzine both of which are inhibitors of monoaminoxidase reactions form red condensation products in their reaction with glutacondialdehyde. The latter compound which was prepared from pyridylpyridinium dichloride and sodium hydroxide has to be in excess concentration. This colour reaction is used in a photometric method to determine 0.6 to 100 nmole/ml of the hydrazine derivatives. The variation coefficients at 2 nmole/ml are 3.2 and 5.2% rel. for benzylhydrazine and phenelzine, respectively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00423799
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    New method for the spectrophotometric determinations of ascorbic acid and dehydroascorbic acid (1947)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 265 (1947), S. 334-336 
    Details
    ISSN: 1618-2650
    Keywords: Best. von Ascorbinsäure und Dehydroascorbinsäure in Pharmazeut. Produkten ; Spektralphotometrie ; Dimethylphenylendiamin als Reagens
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Summary The condensation of dehydroascorbic acid with o-phenylene diamine or its 4,5-dimethyl derivative yields quinoxaline derivatives which can be measured by photometry. Ascorbic acid can be determined after preliminary oxidation with mercury(II) chloride in the same way. This highly specific method has been employed for determinations in pharmaceutical formulations (coefficient of variation ± 1–1.5%).
    Notes: Zusammenfassung Die Kondensation von Dehydroascorbinsäure mit o-Phenylendiamin oder 4,5-Dimethyl-o-phenylendiamin führt zur Bildung von spektrophotometrisch auswertbaren Chinoxalinderivaten. Ascorbinsäure kann nach vorangehender Oxidation mit Quecksilberchlorid auf die gleiche Weise bestimmt werden. Die Methode wurde zu Bestimmungen in pharmazeutischen Präparaten verwendet (Variationskoeffizient ± 1–1,5%).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00423802
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    Paper (German National Licenses)
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  • 7
    Electronic Resource
    Electronic Resource
    Rapid extraction and direct spectrophotometric determination of iron(III) with thiothenoyltrifluoroacetone (1947)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 265 (1947), S. 348-348 
    Details
    ISSN: 1618-2650
    Keywords: Best. von Eisen(III) ; Spektralphotometrie ; Extraktion mit Thiothenoyltrifluoraceton
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00423808
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    Paper (German National Licenses)
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  • 8
    Electronic Resource
    Electronic Resource
    Synergetic extraction and spectrophotometric determination of iron(III) in steel (1947)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 265 (1947), S. 349-349 
    Details
    ISSN: 1618-2650
    Keywords: Best. von Eisen(III) in Stahl ; Spektralphotometrie ; Extraktion mit Acetylaceton und Pyridin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00423809
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    Paper (German National Licenses)
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  • 9
    Electronic Resource
    Electronic Resource
    Extraction of cobalt with ethyl acetate and simultaneous photometric determination of cobalt and nickel (1947)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 265 (1947), S. 351-351 
    Details
    ISSN: 1618-2650
    Keywords: Best. von Kobalt neben Nickel ; Spektralphotometrie ; Extraktion mit Äthylacetat als Xanthate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00423811
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    Paper (German National Licenses)
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